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Article: Characterization of the sprouting response of axon-like processes from retinal ganglion cells after axotomy in adult hamsters: A model using intravitreal implantation of a peripheral nerve

TitleCharacterization of the sprouting response of axon-like processes from retinal ganglion cells after axotomy in adult hamsters: A model using intravitreal implantation of a peripheral nerve
Authors
Issue Date1992
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-4864
Citation
Journal Of Neurocytology, 1992, v. 21 n. 8, p. 589-603 How to Cite?
AbstractPeripheral nerves provide a favourable environment for damaged CNS axons to sprout and regenerate. It has also been demonstrated that retinal ganglion cells respond to a peripheral nerve segment grafted to the retina by emitting axon-like processes from the somatodendritic compartment into the graft. The factors influencing the pattern of sprouting of axotomized retinal ganglion cells were explored in this study by implanting a short segment of peripheral nerve, which did not come into contact with the retina, into the vitreous body of an eye whose optic nerve was concurrently crushed. Silver staining was used to assess the morphology of the retinal ganglion cells which underwent sprouting. Some retinal ganglion cells were induced to sprout axon-like processes; these emerged primarily from dendrites and less frequently from the soma or intraretinal axon. Implantation of a nonviable graft (freezed-thawed) elicited only minimal sprouting. These results suggest that diffusible factors secreted by cells in the graft are a possible stimulus to sprouting in axotomized retinal ganglion cells. Examination of the pattern of dendritic sprouting indicates that sprouting was most intense (in terms of number of sprouts per cell) at early times post-axotomy. Moreover, a differential pattern of development of sprouts arising from individual primary dendrites of the same cell was observed; sprouts tend to arise from all primary dendrites initially but as the post-axotomy time increased, retraction of sprouts from some primary dendrites occurred. Concomitant with this retraction, however, there was an increase in the number of sprouts on those primary dendrites which were still in the active phase of sprouting. Selective stabilization of sprouts by extrinsic factors may account for this phenomenon. Changes in the area and outline (irregularity) of the somata of retinal ganglion cells with sprouts from two weeks to two months after optic nerve crush could be correlated temporally with the intensity of sprouting from the dendritic tree, suggesting that during sprouting, intrinsic mechanisms coordinate the responses of different cellular compartments. In contrast to extensive ectopic sprouting of axotomized retinal ganglion cells in the presence of an intravitreal graft, when a long peripheral nerve segment is grafted to the cut optic nerve, there is extensive axonal regeneration into the graft from retinal ganglion cells, most of which did not exhibit ectopic sprouting. Thus, a hierarchy of sprouting sites within a neuron seems to exist, with the damaged axonal tip being the most favoured site, followed by the dendrites, and then the intraretinal axon. The soma appears to be the least preferred compartment for sprout emission.
Persistent Identifierhttp://hdl.handle.net/10722/67905
ISSN
2007 Impact Factor: 1.935
2009 SCImago Journal Rankings: 0.911
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCho, EYPen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-06T05:59:20Z-
dc.date.available2010-09-06T05:59:20Z-
dc.date.issued1992en_HK
dc.identifier.citationJournal Of Neurocytology, 1992, v. 21 n. 8, p. 589-603en_HK
dc.identifier.issn0300-4864en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67905-
dc.description.abstractPeripheral nerves provide a favourable environment for damaged CNS axons to sprout and regenerate. It has also been demonstrated that retinal ganglion cells respond to a peripheral nerve segment grafted to the retina by emitting axon-like processes from the somatodendritic compartment into the graft. The factors influencing the pattern of sprouting of axotomized retinal ganglion cells were explored in this study by implanting a short segment of peripheral nerve, which did not come into contact with the retina, into the vitreous body of an eye whose optic nerve was concurrently crushed. Silver staining was used to assess the morphology of the retinal ganglion cells which underwent sprouting. Some retinal ganglion cells were induced to sprout axon-like processes; these emerged primarily from dendrites and less frequently from the soma or intraretinal axon. Implantation of a nonviable graft (freezed-thawed) elicited only minimal sprouting. These results suggest that diffusible factors secreted by cells in the graft are a possible stimulus to sprouting in axotomized retinal ganglion cells. Examination of the pattern of dendritic sprouting indicates that sprouting was most intense (in terms of number of sprouts per cell) at early times post-axotomy. Moreover, a differential pattern of development of sprouts arising from individual primary dendrites of the same cell was observed; sprouts tend to arise from all primary dendrites initially but as the post-axotomy time increased, retraction of sprouts from some primary dendrites occurred. Concomitant with this retraction, however, there was an increase in the number of sprouts on those primary dendrites which were still in the active phase of sprouting. Selective stabilization of sprouts by extrinsic factors may account for this phenomenon. Changes in the area and outline (irregularity) of the somata of retinal ganglion cells with sprouts from two weeks to two months after optic nerve crush could be correlated temporally with the intensity of sprouting from the dendritic tree, suggesting that during sprouting, intrinsic mechanisms coordinate the responses of different cellular compartments. In contrast to extensive ectopic sprouting of axotomized retinal ganglion cells in the presence of an intravitreal graft, when a long peripheral nerve segment is grafted to the cut optic nerve, there is extensive axonal regeneration into the graft from retinal ganglion cells, most of which did not exhibit ectopic sprouting. Thus, a hierarchy of sprouting sites within a neuron seems to exist, with the damaged axonal tip being the most favoured site, followed by the dendrites, and then the intraretinal axon. The soma appears to be the least preferred compartment for sprout emission.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-4864en_HK
dc.relation.ispartofJournal of Neurocytologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAxons - physiology - ultrastructureen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshDendrites - physiology - ultrastructureen_HK
dc.subject.meshDenervationen_HK
dc.subject.meshHistocytochemistryen_HK
dc.subject.meshMesocricetusen_HK
dc.subject.meshNerve Crushen_HK
dc.subject.meshOptic Nerve - physiologyen_HK
dc.subject.meshPeripheral Nerves - transplantationen_HK
dc.subject.meshRetinal Ganglion Cells - ultrastructureen_HK
dc.subject.meshSilveren_HK
dc.subject.meshStaining and Labelingen_HK
dc.titleCharacterization of the sprouting response of axon-like processes from retinal ganglion cells after axotomy in adult hamsters: A model using intravitreal implantation of a peripheral nerveen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-4864&volume=21&spage=589&epage=603&date=1992&atitle=Characterization+of+the+sprouting+response+of+axon-like+processes+from+retinal+ganglion+cells+after+axotomy+in+adult+hamsters:+a+model+using+intravitreal+implantation+of+a+peripheral+nerveen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid1380545-
dc.identifier.scopuseid_2-s2.0-0026695421en_HK
dc.identifier.hkuros97745en_HK
dc.identifier.volume21en_HK
dc.identifier.issue8en_HK
dc.identifier.spage589en_HK
dc.identifier.epage603en_HK
dc.identifier.isiWOS:A1992JG76900004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCho, EYP=7202649985en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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