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Article: Identification of RASSF1A modulated genes in nasopharyngeal carcinoma

TitleIdentification of RASSF1A modulated genes in nasopharyngeal carcinoma
Authors
KeywordsId2
Microarray
Nasopharyngeal carcinoma
RASSF1A
Tumor suppressor
Issue Date2006
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2006, v. 25 n. 2, p. 310-316 How to Cite?
AbstractRASSF1A is a tumor suppressor gene on 3p21.3 frequently inactivated by promoter hypermethylation in nasopharyngeal carcinoma (NPC). To identify RASSF1A target genes in NPC, we have investigated the expression profile of the stable RASSF1A transfectants and controls by high-density oligonucleotide array. A total of 57 genes showed differential expression in the RASSF1A-expressing cells. These RASSF1A target genes were involved in multiple cellular regulatory processes such as transcription, signal transduction, cell adhesion and RNA processing. The RASSF1A-modulated expression of eight selected genes with the highest fold changes (ATF5, TCRB, RGS1, activin βE, HNRPH1, HNRPD, Id2 and CKS2) by RASSF1A was confirmed in both stable and transient transfectants. Compared with the RASSF1A transfectants, an inverse expression pattern of activin βE, Id2 and ATF5 was shown in the immortalized nasopharyngeal epithelial cells treated with siRNA against RASSF1A. The findings imply that the expression of activin βE, Id2 and ATF5 was tightly regulated by RASSF1A and may associate with its tumor suppressor function. Strikingly, overexpression of Id2 is common in NPC and RASSF1A-induced repression of Id2 was mediated by the overexpression of activin βE. The results suggest a novel RASSF1A pathway in which both activin βE and Id2 are involved. © 2006 Nature Publishing Group All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67899
ISSN
2015 Impact Factor: 7.932
2015 SCImago Journal Rankings: 4.047
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChow, LSNen_HK
dc.contributor.authorLam, CWen_HK
dc.contributor.authorChan, SYYen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorTo, KFen_HK
dc.contributor.authorTong, SFen_HK
dc.contributor.authorHung, WKen_HK
dc.contributor.authorDammann, Ren_HK
dc.contributor.authorHuang, DPen_HK
dc.contributor.authorLo, KWen_HK
dc.date.accessioned2010-09-06T05:59:17Z-
dc.date.available2010-09-06T05:59:17Z-
dc.date.issued2006en_HK
dc.identifier.citationOncogene, 2006, v. 25 n. 2, p. 310-316en_HK
dc.identifier.issn0950-9232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67899-
dc.description.abstractRASSF1A is a tumor suppressor gene on 3p21.3 frequently inactivated by promoter hypermethylation in nasopharyngeal carcinoma (NPC). To identify RASSF1A target genes in NPC, we have investigated the expression profile of the stable RASSF1A transfectants and controls by high-density oligonucleotide array. A total of 57 genes showed differential expression in the RASSF1A-expressing cells. These RASSF1A target genes were involved in multiple cellular regulatory processes such as transcription, signal transduction, cell adhesion and RNA processing. The RASSF1A-modulated expression of eight selected genes with the highest fold changes (ATF5, TCRB, RGS1, activin βE, HNRPH1, HNRPD, Id2 and CKS2) by RASSF1A was confirmed in both stable and transient transfectants. Compared with the RASSF1A transfectants, an inverse expression pattern of activin βE, Id2 and ATF5 was shown in the immortalized nasopharyngeal epithelial cells treated with siRNA against RASSF1A. The findings imply that the expression of activin βE, Id2 and ATF5 was tightly regulated by RASSF1A and may associate with its tumor suppressor function. Strikingly, overexpression of Id2 is common in NPC and RASSF1A-induced repression of Id2 was mediated by the overexpression of activin βE. The results suggest a novel RASSF1A pathway in which both activin βE and Id2 are involved. © 2006 Nature Publishing Group All rights reserved.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_HK
dc.relation.ispartofOncogeneen_HK
dc.subjectId2en_HK
dc.subjectMicroarrayen_HK
dc.subjectNasopharyngeal carcinomaen_HK
dc.subjectRASSF1Aen_HK
dc.subjectTumor suppressoren_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGene Silencingen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInhibin-beta Subunits - genetics - metabolismen_HK
dc.subject.meshInhibitor of Differentiation Protein 2 - genetics - metabolismen_HK
dc.subject.meshNasopharyngeal Neoplasms - genetics - metabolismen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSignal Transductionen_HK
dc.subject.meshTransfectionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshTumor Markers, Biological - genetics - metabolismen_HK
dc.subject.meshTumor Suppressor Proteins - antagonists & inhibitors - genetics - metabolismen_HK
dc.titleIdentification of RASSF1A modulated genes in nasopharyngeal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-9232&volume=&spage=310&epage=316&date=2005&atitle=Identification+of+RASSF1A+modulated+genes+in+nasopharyngeal+carcinomaen_HK
dc.identifier.emailLam, CW:ching-wanlam@pathology.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityLam, CW=rp00260en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/sj.onc.1209001en_HK
dc.identifier.pmid16116475-
dc.identifier.scopuseid_2-s2.0-30544454169en_HK
dc.identifier.hkuros109869en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-30544454169&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue2en_HK
dc.identifier.spage310en_HK
dc.identifier.epage316en_HK
dc.identifier.isiWOS:000234583600016-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChow, LSN=7202533094en_HK
dc.identifier.scopusauthoridLam, CW=34570692600en_HK
dc.identifier.scopusauthoridChan, SYY=34973871600en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridTo, KF=7101911940en_HK
dc.identifier.scopusauthoridTong, SF=7201486672en_HK
dc.identifier.scopusauthoridHung, WK=13310033500en_HK
dc.identifier.scopusauthoridDammann, R=6701505261en_HK
dc.identifier.scopusauthoridHuang, DP=7403891486en_HK
dc.identifier.scopusauthoridLo, KW=34872774800en_HK
dc.identifier.citeulike307412-

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