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Article: Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity

TitleImmortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity
Authors
Issue Date2006
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 2006, v. 12 n. 7, p. 451-460 How to Cite?
AbstractPlacental trophoblast cells are unique endocrine cells that play vital roles during the processes of embryonic implantation and placentation. However, research into the function of human trophoblast has been largely restrained mainly due to a lack of adequate cell models. A normal placenta-origin cytotrophoblast cell line (NPC) was previously established by our group, but these cells showed replicating senescence after 50 population doublings (PDs). In this study, the human telomerase catalytic subunit gene (htert) was transferred into B6 strain of NPC cells, and strains with reconstituted telomerase activity (B6Tert) were established. It was shown that B6Tert-1 cells produce various biomarkers of normal extravillous cytotrophoblasts during the early weeks of gestation. Meanwhile, the cell invasiveness was inhibited by transforming growth factor β (TGFβ). However, their ability to form syncytium was relatively low when stimulated with fetal calf serum (FCS). The cells maintained normal cell growth properties and failed to elicit tumours in nude mice. They proliferated continuously with no signs of senescence until the final count at 210 PDs. The growth rate of B6Tert-1 cells was increased when compared with the parental cells, which results, at least partly, from facilitating release of the G1/S checkpoint during the cell-cycle regulation. This is the first report of immortalizing human normal cytotrophoblast (CTB) cells by activation of telomerase activity. The cells will provide an ideal in vitro model for the study of human extravillous trophoblast (EVT) functions and consequently the mechanisms of embryonic implantation and placentation. © 2006 Oxford University Press.
Persistent Identifierhttp://hdl.handle.net/10722/67895
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, YLen_HK
dc.contributor.authorQiu, Wen_HK
dc.contributor.authorFeng, HCen_HK
dc.contributor.authorLi, YXen_HK
dc.contributor.authorZhuang, LZen_HK
dc.contributor.authorWang, Zen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorZhou, JQen_HK
dc.contributor.authorZhang, DHen_HK
dc.contributor.authorTsao, GSWen_HK
dc.date.accessioned2010-09-06T05:59:15Z-
dc.date.available2010-09-06T05:59:15Z-
dc.date.issued2006en_HK
dc.identifier.citationMolecular Human Reproduction, 2006, v. 12 n. 7, p. 451-460en_HK
dc.identifier.issn1360-9947en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67895-
dc.description.abstractPlacental trophoblast cells are unique endocrine cells that play vital roles during the processes of embryonic implantation and placentation. However, research into the function of human trophoblast has been largely restrained mainly due to a lack of adequate cell models. A normal placenta-origin cytotrophoblast cell line (NPC) was previously established by our group, but these cells showed replicating senescence after 50 population doublings (PDs). In this study, the human telomerase catalytic subunit gene (htert) was transferred into B6 strain of NPC cells, and strains with reconstituted telomerase activity (B6Tert) were established. It was shown that B6Tert-1 cells produce various biomarkers of normal extravillous cytotrophoblasts during the early weeks of gestation. Meanwhile, the cell invasiveness was inhibited by transforming growth factor β (TGFβ). However, their ability to form syncytium was relatively low when stimulated with fetal calf serum (FCS). The cells maintained normal cell growth properties and failed to elicit tumours in nude mice. They proliferated continuously with no signs of senescence until the final count at 210 PDs. The growth rate of B6Tert-1 cells was increased when compared with the parental cells, which results, at least partly, from facilitating release of the G1/S checkpoint during the cell-cycle regulation. This is the first report of immortalizing human normal cytotrophoblast (CTB) cells by activation of telomerase activity. The cells will provide an ideal in vitro model for the study of human extravillous trophoblast (EVT) functions and consequently the mechanisms of embryonic implantation and placentation. © 2006 Oxford University Press.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_HK
dc.relation.ispartofMolecular Human Reproductionen_HK
dc.rightsMolecular Human Reproduction . Copyright © Oxford University Press.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBlotting, Northernen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshDNA-Binding Proteins - genetics - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshKaryotypingen_HK
dc.subject.meshKeratins - analysisen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshMicroscopy, Phase-Contrast - methodsen_HK
dc.subject.meshNeoplasms, Experimental - enzymology - genetics - pathologyen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshRetinoblastoma Protein - genetics - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTelomerase - genetics - metabolismen_HK
dc.subject.meshTelomere - genetics - metabolismen_HK
dc.subject.meshTransfectionen_HK
dc.subject.meshTrophoblasts - cytology - metabolismen_HK
dc.subject.meshTumor Suppressor Protein p53 - genetics - metabolismen_HK
dc.subject.meshVimentin - analysisen_HK
dc.titleImmortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1360-9947&volume=12&issue=7&spage=451&epage=460&date=2006&atitle=Immortalization+of+normal+human+cytotrophoblast+cells+by+reconstitution+of+telomeric+reverse+transcriptase+activityen_HK
dc.identifier.emailTsao, GSW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/molehr/gal054en_HK
dc.identifier.pmid16772430-
dc.identifier.scopuseid_2-s2.0-33747883862en_HK
dc.identifier.hkuros124513en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33747883862&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue7en_HK
dc.identifier.spage451en_HK
dc.identifier.epage460en_HK
dc.identifier.isiWOS:000239905300006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWang, YL=7601492022en_HK
dc.identifier.scopusauthoridQiu, W=49762028200en_HK
dc.identifier.scopusauthoridFeng, HC=7401736336en_HK
dc.identifier.scopusauthoridLi, YX=7502073507en_HK
dc.identifier.scopusauthoridZhuang, LZ=7102368219en_HK
dc.identifier.scopusauthoridWang, Z=17436466300en_HK
dc.identifier.scopusauthoridLiu, Y=26643293600en_HK
dc.identifier.scopusauthoridZhou, JQ=7405551483en_HK
dc.identifier.scopusauthoridZhang, DH=36124408800en_HK
dc.identifier.scopusauthoridTsao, GSW=7102813116en_HK

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