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- Publisher Website: 10.1016/j.expneurol.2005.08.019
- Scopus: eid_2-s2.0-29144497234
- PMID: 16246329
- WOS: WOS:000234534200009
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Article: Antisense oligos to neuronal nitric oxide synthase aggravate motoneuron death induced by spinal root avulsion in adult rat
Title | Antisense oligos to neuronal nitric oxide synthase aggravate motoneuron death induced by spinal root avulsion in adult rat |
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Authors | |
Keywords | Adult rats Antisense oligos Neuronal nitric oxide synthase Root avulsion Spinal motoneurons |
Issue Date | 2006 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnr |
Citation | Experimental Neurology, 2006, v. 197 n. 1, p. 84-92 How to Cite? |
Abstract | The present study used nitric oxide synthase (nNOS) antisense oligos (nNOS AS-ODN) to assess the role of nNOS in motoneuron death induced by spinal root avulsion. A right seventh cervical (C7) spinal root avulsion was performed on adult male Sprague-Dawley rats. Two weeks later, FITC-labeled random oligos (FITC-R-ODN), nNOS AS-ODN, R-ODN or TE buffer was applied to the lesioned side of the C7 spinal segment and refreshed every 3 days. FITC-R-ODN was first detected inside the injured motoneurons at 10 h, accumulated to a maximum by 24 h and faded out from 72 h. Following avulsion, nNOS AS-ODN decreased the number of nNOS-positive motoneurons in the lesioned segment compared either with buffer (P < 0.001 at 15 days, 3 and 4 weeks post-injury) or with R-ODN control (P = 0.002 at 15 days, P < 0.001 at 3 and 4 weeks post-injury). Interestingly, nNOS AS-ODN also decreased the number of surviving motoneurons compared either with buffer (P = 0.005 at 15 days, P < 0.001 at 3 or 4 weeks) or with R-ODN control (P < 0.001 at 3 or 4 weeks). Meanwhile, there were no significant differences between R-ODN and buffer control either in the number of nNOS-positive motoneurons (P = 0.245 at 15 days, P = 0.089 at 3 weeks and P = 0.162 at 4 weeks) or in the number of surviving motoneurons (P = 0.426 at 15 days, P = 0.321 at 3 weeks or P = 0.344 at 4 weeks). These findings indicate that nNOS AS-ODN, applied from 2 weeks after avulsion, aggravates the motoneuron death due to root avulsion by specifically down-regulating nNOS gene expression and that the expression of nNOS in adult spinal motoneurons in response to root avulsion may play a beneficial role in the survival of injured neurons. © 2005 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/67882 |
ISSN | 2023 Impact Factor: 4.6 2023 SCImago Journal Rankings: 1.552 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Zhou, L | en_HK |
dc.contributor.author | Wu, W | en_HK |
dc.date.accessioned | 2010-09-06T05:59:08Z | - |
dc.date.available | 2010-09-06T05:59:08Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | Experimental Neurology, 2006, v. 197 n. 1, p. 84-92 | en_HK |
dc.identifier.issn | 0014-4886 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67882 | - |
dc.description.abstract | The present study used nitric oxide synthase (nNOS) antisense oligos (nNOS AS-ODN) to assess the role of nNOS in motoneuron death induced by spinal root avulsion. A right seventh cervical (C7) spinal root avulsion was performed on adult male Sprague-Dawley rats. Two weeks later, FITC-labeled random oligos (FITC-R-ODN), nNOS AS-ODN, R-ODN or TE buffer was applied to the lesioned side of the C7 spinal segment and refreshed every 3 days. FITC-R-ODN was first detected inside the injured motoneurons at 10 h, accumulated to a maximum by 24 h and faded out from 72 h. Following avulsion, nNOS AS-ODN decreased the number of nNOS-positive motoneurons in the lesioned segment compared either with buffer (P < 0.001 at 15 days, 3 and 4 weeks post-injury) or with R-ODN control (P = 0.002 at 15 days, P < 0.001 at 3 and 4 weeks post-injury). Interestingly, nNOS AS-ODN also decreased the number of surviving motoneurons compared either with buffer (P = 0.005 at 15 days, P < 0.001 at 3 or 4 weeks) or with R-ODN control (P < 0.001 at 3 or 4 weeks). Meanwhile, there were no significant differences between R-ODN and buffer control either in the number of nNOS-positive motoneurons (P = 0.245 at 15 days, P = 0.089 at 3 weeks and P = 0.162 at 4 weeks) or in the number of surviving motoneurons (P = 0.426 at 15 days, P = 0.321 at 3 weeks or P = 0.344 at 4 weeks). These findings indicate that nNOS AS-ODN, applied from 2 weeks after avulsion, aggravates the motoneuron death due to root avulsion by specifically down-regulating nNOS gene expression and that the expression of nNOS in adult spinal motoneurons in response to root avulsion may play a beneficial role in the survival of injured neurons. © 2005 Elsevier Inc. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnr | en_HK |
dc.relation.ispartof | Experimental Neurology | en_HK |
dc.subject | Adult rats | - |
dc.subject | Antisense oligos | - |
dc.subject | Neuronal nitric oxide synthase | - |
dc.subject | Root avulsion | - |
dc.subject | Spinal motoneurons | - |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Cell Count | en_HK |
dc.subject.mesh | Cell Death - genetics | en_HK |
dc.subject.mesh | Cell Survival - genetics | en_HK |
dc.subject.mesh | Fluorescein-5-isothiocyanate | en_HK |
dc.subject.mesh | Fluorescent Dyes | en_HK |
dc.subject.mesh | Gene Expression Regulation, Enzymologic - genetics | en_HK |
dc.subject.mesh | Immunohistochemistry | en_HK |
dc.subject.mesh | Injections, Spinal | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Motor Neurons - pathology | en_HK |
dc.subject.mesh | NADPH Dehydrogenase - metabolism | en_HK |
dc.subject.mesh | Nitric Oxide Synthase Type I - biosynthesis - genetics | en_HK |
dc.subject.mesh | Oligonucleotides, Antisense - administration & dosage - chemical synthesis - pharmacology | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Rats, Sprague-Dawley | en_HK |
dc.subject.mesh | Spinal Cord - pathology | en_HK |
dc.subject.mesh | Spinal Cord Injuries - pathology | en_HK |
dc.title | Antisense oligos to neuronal nitric oxide synthase aggravate motoneuron death induced by spinal root avulsion in adult rat | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-4886&volume=197&spage=84&epage=92&date=2006&atitle=Antisense+oligos+to+neuronal+nitric+oxide+synthase+aggravate+motoneuron+death+induced+by+spinal+root+avulsion+in+adult+rat | en_HK |
dc.identifier.email | Wu, W:wtwu@hkucc.hku.hk | en_HK |
dc.identifier.authority | Wu, W=rp00419 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.expneurol.2005.08.019 | en_HK |
dc.identifier.pmid | 16246329 | - |
dc.identifier.scopus | eid_2-s2.0-29144497234 | en_HK |
dc.identifier.hkuros | 112941 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-29144497234&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 197 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 84 | en_HK |
dc.identifier.epage | 92 | en_HK |
dc.identifier.isi | WOS:000234534200009 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Zhou, L=7404125592 | en_HK |
dc.identifier.scopusauthorid | Wu, W=7407081122 | en_HK |
dc.identifier.issnl | 0014-4886 | - |