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Article: Antisense oligos to neuronal nitric oxide synthase aggravate motoneuron death induced by spinal root avulsion in adult rat

TitleAntisense oligos to neuronal nitric oxide synthase aggravate motoneuron death induced by spinal root avulsion in adult rat
Authors
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnr
Citation
Experimental Neurology, 2006, v. 197 n. 1, p. 84-92 How to Cite?
AbstractThe present study used nitric oxide synthase (nNOS) antisense oligos (nNOS AS-ODN) to assess the role of nNOS in motoneuron death induced by spinal root avulsion. A right seventh cervical (C7) spinal root avulsion was performed on adult male Sprague-Dawley rats. Two weeks later, FITC-labeled random oligos (FITC-R-ODN), nNOS AS-ODN, R-ODN or TE buffer was applied to the lesioned side of the C7 spinal segment and refreshed every 3 days. FITC-R-ODN was first detected inside the injured motoneurons at 10 h, accumulated to a maximum by 24 h and faded out from 72 h. Following avulsion, nNOS AS-ODN decreased the number of nNOS-positive motoneurons in the lesioned segment compared either with buffer (P < 0.001 at 15 days, 3 and 4 weeks post-injury) or with R-ODN control (P = 0.002 at 15 days, P < 0.001 at 3 and 4 weeks post-injury). Interestingly, nNOS AS-ODN also decreased the number of surviving motoneurons compared either with buffer (P = 0.005 at 15 days, P < 0.001 at 3 or 4 weeks) or with R-ODN control (P < 0.001 at 3 or 4 weeks). Meanwhile, there were no significant differences between R-ODN and buffer control either in the number of nNOS-positive motoneurons (P = 0.245 at 15 days, P = 0.089 at 3 weeks and P = 0.162 at 4 weeks) or in the number of surviving motoneurons (P = 0.426 at 15 days, P = 0.321 at 3 weeks or P = 0.344 at 4 weeks). These findings indicate that nNOS AS-ODN, applied from 2 weeks after avulsion, aggravates the motoneuron death due to root avulsion by specifically down-regulating nNOS gene expression and that the expression of nNOS in adult spinal motoneurons in response to root avulsion may play a beneficial role in the survival of injured neurons. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67882
ISSN
2015 Impact Factor: 4.657
2015 SCImago Journal Rankings: 2.427
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Len_HK
dc.contributor.authorWu, Wen_HK
dc.date.accessioned2010-09-06T05:59:08Z-
dc.date.available2010-09-06T05:59:08Z-
dc.date.issued2006en_HK
dc.identifier.citationExperimental Neurology, 2006, v. 197 n. 1, p. 84-92en_HK
dc.identifier.issn0014-4886en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67882-
dc.description.abstractThe present study used nitric oxide synthase (nNOS) antisense oligos (nNOS AS-ODN) to assess the role of nNOS in motoneuron death induced by spinal root avulsion. A right seventh cervical (C7) spinal root avulsion was performed on adult male Sprague-Dawley rats. Two weeks later, FITC-labeled random oligos (FITC-R-ODN), nNOS AS-ODN, R-ODN or TE buffer was applied to the lesioned side of the C7 spinal segment and refreshed every 3 days. FITC-R-ODN was first detected inside the injured motoneurons at 10 h, accumulated to a maximum by 24 h and faded out from 72 h. Following avulsion, nNOS AS-ODN decreased the number of nNOS-positive motoneurons in the lesioned segment compared either with buffer (P < 0.001 at 15 days, 3 and 4 weeks post-injury) or with R-ODN control (P = 0.002 at 15 days, P < 0.001 at 3 and 4 weeks post-injury). Interestingly, nNOS AS-ODN also decreased the number of surviving motoneurons compared either with buffer (P = 0.005 at 15 days, P < 0.001 at 3 or 4 weeks) or with R-ODN control (P < 0.001 at 3 or 4 weeks). Meanwhile, there were no significant differences between R-ODN and buffer control either in the number of nNOS-positive motoneurons (P = 0.245 at 15 days, P = 0.089 at 3 weeks and P = 0.162 at 4 weeks) or in the number of surviving motoneurons (P = 0.426 at 15 days, P = 0.321 at 3 weeks or P = 0.344 at 4 weeks). These findings indicate that nNOS AS-ODN, applied from 2 weeks after avulsion, aggravates the motoneuron death due to root avulsion by specifically down-regulating nNOS gene expression and that the expression of nNOS in adult spinal motoneurons in response to root avulsion may play a beneficial role in the survival of injured neurons. © 2005 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnren_HK
dc.relation.ispartofExperimental Neurologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Counten_HK
dc.subject.meshCell Death - geneticsen_HK
dc.subject.meshCell Survival - geneticsen_HK
dc.subject.meshFluorescein-5-isothiocyanateen_HK
dc.subject.meshFluorescent Dyesen_HK
dc.subject.meshGene Expression Regulation, Enzymologic - geneticsen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshInjections, Spinalen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMotor Neurons - pathologyen_HK
dc.subject.meshNADPH Dehydrogenase - metabolismen_HK
dc.subject.meshNitric Oxide Synthase Type I - biosynthesis - geneticsen_HK
dc.subject.meshOligonucleotides, Antisense - administration & dosage - chemical synthesis - pharmacologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshSpinal Cord - pathologyen_HK
dc.subject.meshSpinal Cord Injuries - pathologyen_HK
dc.titleAntisense oligos to neuronal nitric oxide synthase aggravate motoneuron death induced by spinal root avulsion in adult raten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-4886&volume=197&spage=84&epage=92&date=2006&atitle=Antisense+oligos+to+neuronal+nitric+oxide+synthase+aggravate+motoneuron+death+induced+by+spinal+root+avulsion+in+adult+raten_HK
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_HK
dc.identifier.authorityWu, W=rp00419en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.expneurol.2005.08.019en_HK
dc.identifier.pmid16246329-
dc.identifier.scopuseid_2-s2.0-29144497234en_HK
dc.identifier.hkuros112941en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-29144497234&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume197en_HK
dc.identifier.issue1en_HK
dc.identifier.spage84en_HK
dc.identifier.epage92en_HK
dc.identifier.isiWOS:000234534200009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhou, L=7404125592en_HK
dc.identifier.scopusauthoridWu, W=7407081122en_HK

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