Article: Inhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cells

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TitleInhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cells
AuthorsPan, NY3
Hui, WS3
Tipoe, GL2
Taylor, GW1
Leung, RYH3
Lam, WK3
Tsang, KWT3
Mak, JCW3
KeywordsBronchiectasis
Dexamethasone
Fluticasone
Interleukin-8
Pseudomonas aeruginosa pyocyanin
Issue Date2006
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
CitationRespiratory Medicine, 2006, v. 100 n. 9, p. 1614-1622 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.rmed.2005.12.003
AbstractAirway epithelial cells are the first targets of environmental stimuli and local cytokines. Pyocyanin-induced synergism with interleukin (IL)-1 or tumour necrosis factor (TNF) in triggering IL-8 release has been documented previously. In this study, IL-8 mRNA and protein expression were examined in cultured human bronchial epithelial cells (BEAS-2B) stimulated with pyocyanin alone, and in combination with IL-1β or phorbol 12,13-dibutyrate (PDBu) in the absence and presence of a group of glucocorticoids. IL-8 mRNA was measured by RT-PCR, and IL-8 protein by ELISA (cell supernatants). Pyocyanin alone produced no increase in IL-8 mRNA and release. However, pyocyanin upregulated the stimulatory effect of IL-1β or PDBu on the release of IL-8 in a dose-dependent manner. The stimulatory effect of pyocyanin on the IL-1β- or PDBu-stimulated IL-8 release was reduced in the presence of dexamethasone, budesonide, and fluticasone. Budesonide and fluticasone were 10-fold more potent than dexamethasone. The protein kinase C (PKC) inhibitor, Go6976, also significantly reduced the stimulatory effect of pyocyanin on IL-1β, and PDBu increased IL-8 release. In conclusion, this study shows that PKC signal pathway seems to be involved in the pyocyanin-mediated upregulation of the IL-1β and PDBu-induced IL-8 release in BEAS-2B cells. These findings suggest that a vicious cycle perpetuating inflammation may exist in the biologic milieu of bronchiectatic patients infected with Pseudomonas aeruginosa due to the production of pyocyanin. The priming action of pyocyanin appears to be blocked by glucocorticoids, thus providing in vitro data in support of the clinical efficacy of inhaled glucocorticoids as anti-inflammatory drugs. © 2006 Elsevier Ltd. All rights reserved.
ISSN0954-6111
2011 Impact Factor: 2.475
2011 SCImago Journal Rankings: 0.213
DOIhttp://dx.doi.org/10.1016/j.rmed.2005.12.003
ISI Accession Number IDWOS:000239833900017
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorPan, NY
dc.contributor.authorHui, WS
dc.contributor.authorTipoe, GL
dc.contributor.authorTaylor, GW
dc.contributor.authorLeung, RYH
dc.contributor.authorLam, WK
dc.contributor.authorTsang, KWT
dc.contributor.authorMak, JCW
dc.date.accessioned2010-09-06T05:59:07Z
dc.date.available2010-09-06T05:59:07Z
dc.date.issued2006
dc.description.abstractAirway epithelial cells are the first targets of environmental stimuli and local cytokines. Pyocyanin-induced synergism with interleukin (IL)-1 or tumour necrosis factor (TNF) in triggering IL-8 release has been documented previously. In this study, IL-8 mRNA and protein expression were examined in cultured human bronchial epithelial cells (BEAS-2B) stimulated with pyocyanin alone, and in combination with IL-1β or phorbol 12,13-dibutyrate (PDBu) in the absence and presence of a group of glucocorticoids. IL-8 mRNA was measured by RT-PCR, and IL-8 protein by ELISA (cell supernatants). Pyocyanin alone produced no increase in IL-8 mRNA and release. However, pyocyanin upregulated the stimulatory effect of IL-1β or PDBu on the release of IL-8 in a dose-dependent manner. The stimulatory effect of pyocyanin on the IL-1β- or PDBu-stimulated IL-8 release was reduced in the presence of dexamethasone, budesonide, and fluticasone. Budesonide and fluticasone were 10-fold more potent than dexamethasone. The protein kinase C (PKC) inhibitor, Go6976, also significantly reduced the stimulatory effect of pyocyanin on IL-1β, and PDBu increased IL-8 release. In conclusion, this study shows that PKC signal pathway seems to be involved in the pyocyanin-mediated upregulation of the IL-1β and PDBu-induced IL-8 release in BEAS-2B cells. These findings suggest that a vicious cycle perpetuating inflammation may exist in the biologic milieu of bronchiectatic patients infected with Pseudomonas aeruginosa due to the production of pyocyanin. The priming action of pyocyanin appears to be blocked by glucocorticoids, thus providing in vitro data in support of the clinical efficacy of inhaled glucocorticoids as anti-inflammatory drugs. © 2006 Elsevier Ltd. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationRespiratory Medicine, 2006, v. 100 n. 9, p. 1614-1622 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.rmed.2005.12.003
dc.identifier.doihttp://dx.doi.org/10.1016/j.rmed.2005.12.003
dc.identifier.epage1622
dc.identifier.hkuros121431
dc.identifier.isiWOS:000239833900017
dc.identifier.issn0954-6111
2011 Impact Factor: 2.475
2011 SCImago Journal Rankings: 0.213
dc.identifier.issue9
dc.identifier.openurl
dc.identifier.pmid16448811
dc.identifier.scopuseid_2-s2.0-33746192142
dc.identifier.spage1614
dc.identifier.urihttp://hdl.handle.net/10722/67880
dc.identifier.volume100
dc.languageeng
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
dc.publisher.placeUnited Kingdom
dc.relation.ispartofRespiratory Medicine
dc.relation.referencesReferences in Scopus
dc.subject.meshAndrostadienes - pharmacology
dc.subject.meshAnti-Inflammatory Agents - pharmacology
dc.subject.meshBronchi - cytology - drug effects - metabolism
dc.subject.meshBudesonide - pharmacology
dc.subject.meshDexamethasone - pharmacology
dc.subject.meshEnzyme-Linked Immunosorbent Assay
dc.subject.meshEpithelial Cells - drug effects - metabolism
dc.subject.meshGlucocorticoids - pharmacology
dc.subject.meshHumans
dc.subject.meshInterleukin-1 - pharmacology
dc.subject.meshInterleukin-8 - secretion
dc.subject.meshPhorbol 12,13-Dibutyrate - pharmacology
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshProtein Kinase C - drug effects - metabolism
dc.subject.meshPyocyanine - pharmacology
dc.subject.meshRNA, Messenger - drug effects - metabolism
dc.subjectBronchiectasis
dc.subjectDexamethasone
dc.subjectFluticasone
dc.subjectInterleukin-8
dc.subjectPseudomonas aeruginosa pyocyanin
dc.titleInhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cells
dc.typeArticle
Author Affiliations
  1. UCL
  2. The University of Hong Kong
  3. Laboratory Block