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Article: Inhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cells

TitleInhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cells
Authors
KeywordsBronchiectasis
Dexamethasone
Fluticasone
Interleukin-8
Pseudomonas aeruginosa pyocyanin
Issue Date2006
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
Citation
Respiratory Medicine, 2006, v. 100 n. 9, p. 1614-1622 How to Cite?
Abstract
Airway epithelial cells are the first targets of environmental stimuli and local cytokines. Pyocyanin-induced synergism with interleukin (IL)-1 or tumour necrosis factor (TNF) in triggering IL-8 release has been documented previously. In this study, IL-8 mRNA and protein expression were examined in cultured human bronchial epithelial cells (BEAS-2B) stimulated with pyocyanin alone, and in combination with IL-1β or phorbol 12,13-dibutyrate (PDBu) in the absence and presence of a group of glucocorticoids. IL-8 mRNA was measured by RT-PCR, and IL-8 protein by ELISA (cell supernatants). Pyocyanin alone produced no increase in IL-8 mRNA and release. However, pyocyanin upregulated the stimulatory effect of IL-1β or PDBu on the release of IL-8 in a dose-dependent manner. The stimulatory effect of pyocyanin on the IL-1β- or PDBu-stimulated IL-8 release was reduced in the presence of dexamethasone, budesonide, and fluticasone. Budesonide and fluticasone were 10-fold more potent than dexamethasone. The protein kinase C (PKC) inhibitor, Go6976, also significantly reduced the stimulatory effect of pyocyanin on IL-1β, and PDBu increased IL-8 release. In conclusion, this study shows that PKC signal pathway seems to be involved in the pyocyanin-mediated upregulation of the IL-1β and PDBu-induced IL-8 release in BEAS-2B cells. These findings suggest that a vicious cycle perpetuating inflammation may exist in the biologic milieu of bronchiectatic patients infected with Pseudomonas aeruginosa due to the production of pyocyanin. The priming action of pyocyanin appears to be blocked by glucocorticoids, thus providing in vitro data in support of the clinical efficacy of inhaled glucocorticoids as anti-inflammatory drugs. © 2006 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67880
ISSN
2013 Impact Factor: 2.917
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPan, NYen_HK
dc.contributor.authorHui, WSen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorTaylor, GWen_HK
dc.contributor.authorLeung, RYHen_HK
dc.contributor.authorLam, WKen_HK
dc.contributor.authorTsang, KWTen_HK
dc.contributor.authorMak, JCWen_HK
dc.date.accessioned2010-09-06T05:59:07Z-
dc.date.available2010-09-06T05:59:07Z-
dc.date.issued2006en_HK
dc.identifier.citationRespiratory Medicine, 2006, v. 100 n. 9, p. 1614-1622en_HK
dc.identifier.issn0954-6111en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67880-
dc.description.abstractAirway epithelial cells are the first targets of environmental stimuli and local cytokines. Pyocyanin-induced synergism with interleukin (IL)-1 or tumour necrosis factor (TNF) in triggering IL-8 release has been documented previously. In this study, IL-8 mRNA and protein expression were examined in cultured human bronchial epithelial cells (BEAS-2B) stimulated with pyocyanin alone, and in combination with IL-1β or phorbol 12,13-dibutyrate (PDBu) in the absence and presence of a group of glucocorticoids. IL-8 mRNA was measured by RT-PCR, and IL-8 protein by ELISA (cell supernatants). Pyocyanin alone produced no increase in IL-8 mRNA and release. However, pyocyanin upregulated the stimulatory effect of IL-1β or PDBu on the release of IL-8 in a dose-dependent manner. The stimulatory effect of pyocyanin on the IL-1β- or PDBu-stimulated IL-8 release was reduced in the presence of dexamethasone, budesonide, and fluticasone. Budesonide and fluticasone were 10-fold more potent than dexamethasone. The protein kinase C (PKC) inhibitor, Go6976, also significantly reduced the stimulatory effect of pyocyanin on IL-1β, and PDBu increased IL-8 release. In conclusion, this study shows that PKC signal pathway seems to be involved in the pyocyanin-mediated upregulation of the IL-1β and PDBu-induced IL-8 release in BEAS-2B cells. These findings suggest that a vicious cycle perpetuating inflammation may exist in the biologic milieu of bronchiectatic patients infected with Pseudomonas aeruginosa due to the production of pyocyanin. The priming action of pyocyanin appears to be blocked by glucocorticoids, thus providing in vitro data in support of the clinical efficacy of inhaled glucocorticoids as anti-inflammatory drugs. © 2006 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmeden_HK
dc.relation.ispartofRespiratory Medicineen_HK
dc.subjectBronchiectasisen_HK
dc.subjectDexamethasoneen_HK
dc.subjectFluticasoneen_HK
dc.subjectInterleukin-8en_HK
dc.subjectPseudomonas aeruginosa pyocyaninen_HK
dc.subject.meshAndrostadienes - pharmacologyen_HK
dc.subject.meshAnti-Inflammatory Agents - pharmacologyen_HK
dc.subject.meshBronchi - cytology - drug effects - metabolismen_HK
dc.subject.meshBudesonide - pharmacologyen_HK
dc.subject.meshDexamethasone - pharmacologyen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshEpithelial Cells - drug effects - metabolismen_HK
dc.subject.meshGlucocorticoids - pharmacologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInterleukin-1 - pharmacologyen_HK
dc.subject.meshInterleukin-8 - secretionen_HK
dc.subject.meshPhorbol 12,13-Dibutyrate - pharmacologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshProtein Kinase C - drug effects - metabolismen_HK
dc.subject.meshPyocyanine - pharmacologyen_HK
dc.subject.meshRNA, Messenger - drug effects - metabolismen_HK
dc.titleInhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0954-6111&volume=100&issue=9&spage=1614&epage=1622&date=2006&atitle=Inhibition+of+pyocyanin-potentiated+IL-8+release+by+steroids+in+bronchial+epithelial+cellsen_HK
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_HK
dc.identifier.emailMak, JCW:judymak@hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityMak, JCW=rp00352en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.rmed.2005.12.003en_HK
dc.identifier.pmid16448811en_HK
dc.identifier.scopuseid_2-s2.0-33746192142en_HK
dc.identifier.hkuros121431en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746192142&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume100en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1614en_HK
dc.identifier.epage1622en_HK
dc.identifier.isiWOS:000239833900017-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPan, NY=49763709100en_HK
dc.identifier.scopusauthoridHui, WS=49763343700en_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.scopusauthoridTaylor, GW=35493762000en_HK
dc.identifier.scopusauthoridLeung, RYH=7101876102en_HK
dc.identifier.scopusauthoridLam, WK=7203021937en_HK
dc.identifier.scopusauthoridTsang, KWT=7201555024en_HK
dc.identifier.scopusauthoridMak, JCW=7103323094en_HK

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