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Article: Retrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculus

TitleRetrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculus
Authors
KeywordsExperimental glaucoma
Issue 16
Neuroscience
Ophthalmology research
Retinal ganglion cells
Retrograde labeling
Superior colliculus
Issue Date2008
PublisherJournal of Visualized Experiments. The Journal's web site is located at http://wwwjovecom
Citation
Journal Of Visualized Experiments, 2008 n. 16, article no. e819 How to Cite?
AbstractRetinal ganglion cell (RGC) counting is essential to evaluate retinal degeneration especially in glaucoma. Reliable RGC labeling is fundamental for evaluating the effects of any treatment. In rat, about 98% of RGCs is known to project to the contra lateral superior calicles (SC) (Forrester and Peters, 1967). Applying flour-gold (FG) on the surface of SC can label almost all the RGCs, so that we can focus on this most vulnerable retinal neuron in glaucoma. FG is taken up by the axon terminals of retinal ganglion cells and bilaterally transported retrogradely to its somas in the retina. Compare with retrograde labeling of RGC by putting FG at stump of transected optic nerve for 2 days, the interference of RGC survival is minimized. Compare with cresyl violet staining that stains RGCs, amacrine cells and endothelium of the blood vessel in the retinal ganglion cell layer, this labeling method is more specific to the RGC. This video describes the method of retrograde labeling of RGC by applying FG on the surface of SC. The surgical procedures include drilling the skull; aspirating the cortex to expose the SC and applying gelatin sponge over entire dorsal surface of SC are shown. Useful tips for avoiding massive intracranial bleeding and aspiration of the SC have been given. © JoVE 2006-2011 All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67867
ISSN
2015 Impact Factor: 1.113
2015 SCImago Journal Rankings: 0.451
References

 

DC FieldValueLanguage
dc.contributor.authorChiu, Ken_HK
dc.contributor.authorLau, WMen_HK
dc.contributor.authorYeung, SCen_HK
dc.contributor.authorChang, RCCen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-06T05:59:00Z-
dc.date.available2010-09-06T05:59:00Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Visualized Experiments, 2008 n. 16, article no. e819en_HK
dc.identifier.issn1940-087Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67867-
dc.description.abstractRetinal ganglion cell (RGC) counting is essential to evaluate retinal degeneration especially in glaucoma. Reliable RGC labeling is fundamental for evaluating the effects of any treatment. In rat, about 98% of RGCs is known to project to the contra lateral superior calicles (SC) (Forrester and Peters, 1967). Applying flour-gold (FG) on the surface of SC can label almost all the RGCs, so that we can focus on this most vulnerable retinal neuron in glaucoma. FG is taken up by the axon terminals of retinal ganglion cells and bilaterally transported retrogradely to its somas in the retina. Compare with retrograde labeling of RGC by putting FG at stump of transected optic nerve for 2 days, the interference of RGC survival is minimized. Compare with cresyl violet staining that stains RGCs, amacrine cells and endothelium of the blood vessel in the retinal ganglion cell layer, this labeling method is more specific to the RGC. This video describes the method of retrograde labeling of RGC by applying FG on the surface of SC. The surgical procedures include drilling the skull; aspirating the cortex to expose the SC and applying gelatin sponge over entire dorsal surface of SC are shown. Useful tips for avoiding massive intracranial bleeding and aspiration of the SC have been given. © JoVE 2006-2011 All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherJournal of Visualized Experiments. The Journal's web site is located at http://wwwjovecomen_HK
dc.relation.ispartofJournal of Visualized Experimentsen_HK
dc.subjectExperimental glaucomaen_HK
dc.subjectIssue 16en_HK
dc.subjectNeuroscienceen_HK
dc.subjectOphthalmology researchen_HK
dc.subjectRetinal ganglion cellsen_HK
dc.subjectRetrograde labelingen_HK
dc.subjectSuperior colliculusen_HK
dc.titleRetrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1940-087X&volume=16&spage=&epage=&date=2008&atitle=Retrograde+labeling+of+retinal+ganglion+cells+by+application+of+fluoro-gold+on+the+surface+of+superior+colliculusen_HK
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3791/819en_HK
dc.identifier.pmid19066544-
dc.identifier.scopuseid_2-s2.0-80355135085en_HK
dc.identifier.hkuros155147en_HK
dc.identifier.hkuros145259-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80355135085&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.issue16en_HK
dc.identifier.spagearticle no. e819-
dc.identifier.epagearticle no. e819-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChiu, K=15076970500en_HK
dc.identifier.scopusauthoridLau, WM=16239172000en_HK
dc.identifier.scopusauthoridYeung, SC=25923636600en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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