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- Scopus: eid_2-s2.0-80355135085
- PMID: 19066544
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Article: Retrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculus
Title | Retrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculus |
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Authors | |
Keywords | Experimental glaucoma Issue 16 Neuroscience Ophthalmology research Retinal ganglion cells Retrograde labeling Superior colliculus |
Issue Date | 2008 |
Publisher | Journal of Visualized Experiments. The Journal's web site is located at http://www.jove.com |
Citation | Journal of Visualized Experiments, 2008, n. 16, article no. e819 How to Cite? |
Abstract | Retinal ganglion cell (RGC) counting is essential to evaluate retinal degeneration especially in glaucoma. Reliable RGC labeling is fundamental for evaluating the effects of any treatment. In rat, about 98% of RGCs is known to project to the contra lateral superior calicles (SC) (Forrester and Peters, 1967). Applying flour-gold (FG) on the surface of SC can label almost all the RGCs, so that we can focus on this most vulnerable retinal neuron in glaucoma. FG is taken up by the axon terminals of retinal ganglion cells and bilaterally transported retrogradely to its somas in the retina. Compare with retrograde labeling of RGC by putting FG at stump of transected optic nerve for 2 days, the interference of RGC survival is minimized. Compare with cresyl violet staining that stains RGCs, amacrine cells and endothelium of the blood vessel in the retinal ganglion cell layer, this labeling method is more specific to the RGC. This video describes the method of retrograde labeling of RGC by applying FG on the surface of SC. The surgical procedures include drilling the skull; aspirating the cortex to expose the SC and applying gelatin sponge over entire dorsal surface of SC are shown. Useful tips for avoiding massive intracranial bleeding and aspiration of the SC have been given. © JoVE 2006-2011 All Rights Reserved. |
Persistent Identifier | http://hdl.handle.net/10722/67867 |
ISSN | 2023 Impact Factor: 1.2 2023 SCImago Journal Rankings: 0.449 |
PubMed Central ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Chiu, K | en_HK |
dc.contributor.author | Lau, WM | en_HK |
dc.contributor.author | Yeung, SC | en_HK |
dc.contributor.author | Chang, RCC | en_HK |
dc.contributor.author | So, KF | en_HK |
dc.date.accessioned | 2010-09-06T05:59:00Z | - |
dc.date.available | 2010-09-06T05:59:00Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Journal of Visualized Experiments, 2008, n. 16, article no. e819 | en_HK |
dc.identifier.issn | 1940-087X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67867 | - |
dc.description.abstract | Retinal ganglion cell (RGC) counting is essential to evaluate retinal degeneration especially in glaucoma. Reliable RGC labeling is fundamental for evaluating the effects of any treatment. In rat, about 98% of RGCs is known to project to the contra lateral superior calicles (SC) (Forrester and Peters, 1967). Applying flour-gold (FG) on the surface of SC can label almost all the RGCs, so that we can focus on this most vulnerable retinal neuron in glaucoma. FG is taken up by the axon terminals of retinal ganglion cells and bilaterally transported retrogradely to its somas in the retina. Compare with retrograde labeling of RGC by putting FG at stump of transected optic nerve for 2 days, the interference of RGC survival is minimized. Compare with cresyl violet staining that stains RGCs, amacrine cells and endothelium of the blood vessel in the retinal ganglion cell layer, this labeling method is more specific to the RGC. This video describes the method of retrograde labeling of RGC by applying FG on the surface of SC. The surgical procedures include drilling the skull; aspirating the cortex to expose the SC and applying gelatin sponge over entire dorsal surface of SC are shown. Useful tips for avoiding massive intracranial bleeding and aspiration of the SC have been given. © JoVE 2006-2011 All Rights Reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Journal of Visualized Experiments. The Journal's web site is located at http://www.jove.com | en_HK |
dc.relation.ispartof | Journal of Visualized Experiments | en_HK |
dc.subject | Experimental glaucoma | en_HK |
dc.subject | Issue 16 | en_HK |
dc.subject | Neuroscience | en_HK |
dc.subject | Ophthalmology research | en_HK |
dc.subject | Retinal ganglion cells | en_HK |
dc.subject | Retrograde labeling | en_HK |
dc.subject | Superior colliculus | en_HK |
dc.title | Retrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculus | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Chang, RCC:rccchang@hkucc.hku.hk | en_HK |
dc.identifier.email | So, KF:hrmaskf@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chang, RCC=rp00470 | en_HK |
dc.identifier.authority | So, KF=rp00329 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.3791/819 | en_HK |
dc.identifier.pmid | 19066544 | - |
dc.identifier.pmcid | PMC2583033 | - |
dc.identifier.scopus | eid_2-s2.0-80355135085 | en_HK |
dc.identifier.hkuros | 155147 | en_HK |
dc.identifier.hkuros | 145259 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-80355135085&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.issue | 16 | en_HK |
dc.identifier.spage | article no. e819 | - |
dc.identifier.epage | article no. e819 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Chiu, K=15076970500 | en_HK |
dc.identifier.scopusauthorid | Lau, WM=16239172000 | en_HK |
dc.identifier.scopusauthorid | Yeung, SC=25923636600 | en_HK |
dc.identifier.scopusauthorid | Chang, RCC=7403713410 | en_HK |
dc.identifier.scopusauthorid | So, KF=34668391300 | en_HK |
dc.identifier.issnl | 1940-087X | - |