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Article: Cytotoxic effect of gossypol on colon carcinoma cells

TitleCytotoxic effect of gossypol on colon carcinoma cells
Authors
KeywordsApoptosis
Colon carcinoma cells
Gossypol
Mitochondria
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2000, v. 67 n. 22, p. 2663-2671 How to Cite?
AbstractGossypol, a male contraceptive drug extracted from cottonseeds, has been found to have antiproliferative activity on tumour cells and is thought to be a potential anticancer drug. The aim of this study was to investigate the mechanisms of gossypol-induced cell death on two colon carcinoma cell lines, HT29 and LoVo. Firstly, we studied the effect of gossypol on the colony forming ability of these tumour cells, which is the main target of chemotherapeutic drugs. Using clonogenic assays, flow cytometry and DNA gel electrophoresis techniques, we have found that gossypol not only inhibited colony forming ability of these tumour cells, but we also observed cellular internucleosomal DNA fragmentation in the cells treated with 3 doses of gossypol and this was accompanied by the appearance of a sub-G1 apoptotic peak and morphological characteristics of apoptosis. Our results suggest that the gossypol induced cell death is via an apoptotic pathway and the effect of gossypol may not be cell cycle specific. Using Western blotting analysis, we found that the gossypol-induced apoptosis may not be involved in the regulation of p53 but possibly associated with the regulation of bcl-2 and Bax expression. Our evidence indicates that gossypol may provide a potential therapeutic benefit for the treatment of colon carcinoma and understanding the mechanisms of gossypol-induced cytotoxicity on tumour cells is essential for including this drug in clinical use. (C) 2000 Elsevier Science Inc.
Persistent Identifierhttp://hdl.handle.net/10722/67864
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorWong, SCHen_HK
dc.contributor.authorChow, LSNen_HK
dc.contributor.authorNicholls, JMen_HK
dc.contributor.authorWong, YCen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorTsao, SWen_HK
dc.date.accessioned2010-09-06T05:58:58Z-
dc.date.available2010-09-06T05:58:58Z-
dc.date.issued2000en_HK
dc.identifier.citationLife Sciences, 2000, v. 67 n. 22, p. 2663-2671en_HK
dc.identifier.issn0024-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67864-
dc.description.abstractGossypol, a male contraceptive drug extracted from cottonseeds, has been found to have antiproliferative activity on tumour cells and is thought to be a potential anticancer drug. The aim of this study was to investigate the mechanisms of gossypol-induced cell death on two colon carcinoma cell lines, HT29 and LoVo. Firstly, we studied the effect of gossypol on the colony forming ability of these tumour cells, which is the main target of chemotherapeutic drugs. Using clonogenic assays, flow cytometry and DNA gel electrophoresis techniques, we have found that gossypol not only inhibited colony forming ability of these tumour cells, but we also observed cellular internucleosomal DNA fragmentation in the cells treated with 3 doses of gossypol and this was accompanied by the appearance of a sub-G1 apoptotic peak and morphological characteristics of apoptosis. Our results suggest that the gossypol induced cell death is via an apoptotic pathway and the effect of gossypol may not be cell cycle specific. Using Western blotting analysis, we found that the gossypol-induced apoptosis may not be involved in the regulation of p53 but possibly associated with the regulation of bcl-2 and Bax expression. Our evidence indicates that gossypol may provide a potential therapeutic benefit for the treatment of colon carcinoma and understanding the mechanisms of gossypol-induced cytotoxicity on tumour cells is essential for including this drug in clinical use. (C) 2000 Elsevier Science Inc.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_HK
dc.relation.ispartofLife Sciencesen_HK
dc.rightsLife Sciences. Copyright © Elsevier Inc.en_HK
dc.subjectApoptosisen_HK
dc.subjectColon carcinoma cellsen_HK
dc.subjectGossypolen_HK
dc.subjectMitochondriaen_HK
dc.subject.meshCell Cycle - drug effectsen_HK
dc.subject.meshCell Death - drug effectsen_HK
dc.subject.meshCell Survival - drug effectsen_HK
dc.subject.meshClone Cellsen_HK
dc.subject.meshColonic Neoplasms - pathologyen_HK
dc.subject.meshContraceptive Agents, Male - toxicityen_HK
dc.subject.meshDNA Fragmentation - drug effectsen_HK
dc.subject.meshGossypol - toxicityen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKineticsen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleCytotoxic effect of gossypol on colon carcinoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0024-3205&volume=67&spage=2663&epage=2671&date=2000&atitle=Cytotoxic+effect+of+gossypol+on+colon+carcinoma+cellsen_HK
dc.identifier.emailNicholls, JM:nicholls@pathology.hku.hken_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.emailKwong, DLW:dlwkwong@hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityNicholls, JM=rp00364en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11105982-
dc.identifier.scopuseid_2-s2.0-0034692871en_HK
dc.identifier.hkuros56136en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034692871&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume67en_HK
dc.identifier.issue22en_HK
dc.identifier.spage2663en_HK
dc.identifier.epage2671en_HK
dc.identifier.isiWOS:000090043600001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, X=7501854829en_HK
dc.identifier.scopusauthoridWang, J=7701342266en_HK
dc.identifier.scopusauthoridWong, SCH=7404590793en_HK
dc.identifier.scopusauthoridChow, LSN=7202533094en_HK
dc.identifier.scopusauthoridNicholls, JM=7201463077en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK
dc.identifier.scopusauthoridLiu, Y=26643293600en_HK
dc.identifier.scopusauthoridKwong, DLW=15744231600en_HK
dc.identifier.scopusauthoridSham, JST=24472255400en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK

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