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Article: Characterization of human ovarian surface epithelial cells immortalized by human papilloma viral oncogenes (HPV-E6E7 ORFs)

TitleCharacterization of human ovarian surface epithelial cells immortalized by human papilloma viral oncogenes (HPV-E6E7 ORFs)
Authors
Issue Date1995
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr
Citation
Experimental Cell Research, 1995, v. 218 n. 2, p. 499-507 How to Cite?
AbstractPrimary human ovarian surface epithelial (HOSE) cells were immortalized by a retroviral vector (LXSN-16E6E7) expressing HPV-E6E7 open reading frames (ORF). Immortalizations of primary ovarian epithelial cells were achieved in three of three attempts. Detailed analysis was carried out in one line, HOSE 6-3, selected on the basis of its epithelial morphology. The immortalized line (HOSE 6-3) was nontumorigenic in nude mice when examined at subculture number 20. Cytogenetic analysis confirmed its human origin and detailed karyotypic analysis revealed a mixed karyotype made up of about 60% of diploid and 40% of near-tetraploid cells. Clonal chromosomal aberration was observed in a subpopulation of cells involving a ring chromosome number 9. Immunofluorescence and two-dimensional gel electrophoresis revealed the presence of vimentin and several species of cytokeratin (K7, K8, K18, K19). The profile of the cytoskeletal filaments of HOSE 6-3 cells is largely identical with that of normal ovarian epithelial cells before immortalization. The immortalized ovarian epithelial cells have a lower sensitivity to TGF-β1 inhibition compared to normal ovarian epithelial cells. The immortalized line, HOSE 6-3, has altered growth properties including a higher proliferation rate, plating efficiency, and saturation density. The establishment of a continuous line of human ovarian epithelial cells may provide an in vitro model for study of carcinogenesis in human ovarian cancers.
Persistent Identifierhttp://hdl.handle.net/10722/67848
ISSN
2015 Impact Factor: 3.378
2015 SCImago Journal Rankings: 1.900
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorMok, SCen_HK
dc.contributor.authorFey, EGen_HK
dc.contributor.authorFletcher, JAen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorChew, ECen_HK
dc.contributor.authorMuto, MGen_HK
dc.contributor.authorKnapp, RCen_HK
dc.contributor.authorBerkowitz, RSen_HK
dc.date.accessioned2010-09-06T05:58:49Z-
dc.date.available2010-09-06T05:58:49Z-
dc.date.issued1995en_HK
dc.identifier.citationExperimental Cell Research, 1995, v. 218 n. 2, p. 499-507en_HK
dc.identifier.issn0014-4827en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67848-
dc.description.abstractPrimary human ovarian surface epithelial (HOSE) cells were immortalized by a retroviral vector (LXSN-16E6E7) expressing HPV-E6E7 open reading frames (ORF). Immortalizations of primary ovarian epithelial cells were achieved in three of three attempts. Detailed analysis was carried out in one line, HOSE 6-3, selected on the basis of its epithelial morphology. The immortalized line (HOSE 6-3) was nontumorigenic in nude mice when examined at subculture number 20. Cytogenetic analysis confirmed its human origin and detailed karyotypic analysis revealed a mixed karyotype made up of about 60% of diploid and 40% of near-tetraploid cells. Clonal chromosomal aberration was observed in a subpopulation of cells involving a ring chromosome number 9. Immunofluorescence and two-dimensional gel electrophoresis revealed the presence of vimentin and several species of cytokeratin (K7, K8, K18, K19). The profile of the cytoskeletal filaments of HOSE 6-3 cells is largely identical with that of normal ovarian epithelial cells before immortalization. The immortalized ovarian epithelial cells have a lower sensitivity to TGF-β1 inhibition compared to normal ovarian epithelial cells. The immortalized line, HOSE 6-3, has altered growth properties including a higher proliferation rate, plating efficiency, and saturation density. The establishment of a continuous line of human ovarian epithelial cells may provide an in vitro model for study of carcinogenesis in human ovarian cancers.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcren_HK
dc.relation.ispartofExperimental Cell Researchen_HK
dc.subject.meshCell Division - drug effectsen_HK
dc.subject.meshCell Transformation, Viralen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDNA-Binding Proteinsen_HK
dc.subject.meshEpithelium - metabolism - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshGene Transfer Techniquesen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntermediate Filaments - metabolismen_HK
dc.subject.meshKaryotypingen_HK
dc.subject.meshOncogene Proteins, Viral - geneticsen_HK
dc.subject.meshOvary - pathologyen_HK
dc.subject.meshTransforming Growth Factor beta - pharmacologyen_HK
dc.titleCharacterization of human ovarian surface epithelial cells immortalized by human papilloma viral oncogenes (HPV-E6E7 ORFs)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-4827&volume=218&spage=499&epage=507&date=1995&atitle=Characterization+of+human+ovarian+surface+epithelial+cells+immortalized+by+human+papilloma+viral+oncogenes+(HPV-E6E7+ORFs)en_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/excr.1995.1184en_HK
dc.identifier.pmid7796885-
dc.identifier.scopuseid_2-s2.0-0029050422en_HK
dc.identifier.hkuros4695en_HK
dc.identifier.volume218en_HK
dc.identifier.issue2en_HK
dc.identifier.spage499en_HK
dc.identifier.epage507en_HK
dc.identifier.isiWOS:A1995RD63900012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f100013357058-
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridMok, SC=7006015487en_HK
dc.identifier.scopusauthoridFey, EG=6604019658en_HK
dc.identifier.scopusauthoridFletcher, JA=7402937532en_HK
dc.identifier.scopusauthoridWan, TSK=25623981600en_HK
dc.identifier.scopusauthoridChew, EC=7102013767en_HK
dc.identifier.scopusauthoridMuto, MG=7102795179en_HK
dc.identifier.scopusauthoridKnapp, RC=7201853287en_HK
dc.identifier.scopusauthoridBerkowitz, RS=7201352221en_HK

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