File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Effects of male accessory sex glands on sperm decondensation and oocyte activation during in vivo fertilization in golden hamsters

TitleEffects of male accessory sex glands on sperm decondensation and oocyte activation during in vivo fertilization in golden hamsters
Authors
Issue Date1999
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJA
Citation
International Journal Of Andrology, 1999, v. 22 n. 2, p. 68-76 How to Cite?
AbstractRemoval of paternal male accessory sex glands (ASG) could cause a delay in DNA synthesis in hamster zygotes fertilized in vivo. In view of the fact that this process is closely related to pronuclear development which, in part, depends on sperm nuclear decondensation and oocyte activation during fertilization, we carried out a series of experiments were undertaken to determine whether ASG also has an effect on these early events. (1) Oocytes were collected from females mated with SH (sham-operated control), AGX (bilateral excision of ampullary glands), VPX (bilateral excision of ventral prostates) or TX (excision of all ASG) males (n = 8 per group) at 4, 5 and 6 post coitus. (2) Epididymal spermatozoa were incubated with total ventral prostate (VP) secretion to study its effect on dithiothreitol-induced sperm decondensation. (3) Histone H1 kinase activity in oocytes collected as described in (1) was determined. (4) Exocytosed cortical granules on oocytes were labelled with FITC-LCA and quantified by a Metamorph Imaging System. Results showed that sperm decondensation and resumption of meiosis in oocytes in VPX and TX groups were significantly slower compared with SH. VP secretion augmented sperm decondensation in vitro. At 4 hr post coitus, the relative activity of histone H1 kinase in the TX and VPX groups was significantly higher than that in the SH group (p < 0.01). Cortical granule exocytosis in the AGX groups was consistently weaker at all time points studied and was significantly lower than that of the control at 4 hr post coitus (p < 0.05), while the percentage of polyspermic fertilization in the AGX group was significantly higher compared with that in the SH group (p < 0.05). Taken together, these results show that the lack of exposure of spermatozoa to secretions of the ASG does not jeopardize their ability to penetrate ova, although other aspects of their function in the early stages of gamete interaction and subsequent initiation of embryonic development are affected.
Persistent Identifierhttp://hdl.handle.net/10722/67830
ISSN
2014 Impact Factor: 3.695
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYing, Yen_HK
dc.contributor.authorCheung, MPLen_HK
dc.contributor.authorChow, PHen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-09-06T05:58:38Z-
dc.date.available2010-09-06T05:58:38Z-
dc.date.issued1999en_HK
dc.identifier.citationInternational Journal Of Andrology, 1999, v. 22 n. 2, p. 68-76en_HK
dc.identifier.issn0105-6263en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67830-
dc.description.abstractRemoval of paternal male accessory sex glands (ASG) could cause a delay in DNA synthesis in hamster zygotes fertilized in vivo. In view of the fact that this process is closely related to pronuclear development which, in part, depends on sperm nuclear decondensation and oocyte activation during fertilization, we carried out a series of experiments were undertaken to determine whether ASG also has an effect on these early events. (1) Oocytes were collected from females mated with SH (sham-operated control), AGX (bilateral excision of ampullary glands), VPX (bilateral excision of ventral prostates) or TX (excision of all ASG) males (n = 8 per group) at 4, 5 and 6 post coitus. (2) Epididymal spermatozoa were incubated with total ventral prostate (VP) secretion to study its effect on dithiothreitol-induced sperm decondensation. (3) Histone H1 kinase activity in oocytes collected as described in (1) was determined. (4) Exocytosed cortical granules on oocytes were labelled with FITC-LCA and quantified by a Metamorph Imaging System. Results showed that sperm decondensation and resumption of meiosis in oocytes in VPX and TX groups were significantly slower compared with SH. VP secretion augmented sperm decondensation in vitro. At 4 hr post coitus, the relative activity of histone H1 kinase in the TX and VPX groups was significantly higher than that in the SH group (p < 0.01). Cortical granule exocytosis in the AGX groups was consistently weaker at all time points studied and was significantly lower than that of the control at 4 hr post coitus (p < 0.05), while the percentage of polyspermic fertilization in the AGX group was significantly higher compared with that in the SH group (p < 0.05). Taken together, these results show that the lack of exposure of spermatozoa to secretions of the ASG does not jeopardize their ability to penetrate ova, although other aspects of their function in the early stages of gamete interaction and subsequent initiation of embryonic development are affected.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJAen_HK
dc.relation.ispartofInternational Journal of Andrologyen_HK
dc.rightsInternational Journal of Andrology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Nucleusen_HK
dc.subject.meshChromatinen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshExocytosisen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFertilization - physiologyen_HK
dc.subject.meshGenitalia, Male - physiologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMesocricetusen_HK
dc.subject.meshOocytes - physiologyen_HK
dc.subject.meshProtein Kinases - metabolismen_HK
dc.subject.meshSperm-Ovum Interactions - physiologyen_HK
dc.subject.meshSpermatozoa - physiologyen_HK
dc.titleEffects of male accessory sex glands on sperm decondensation and oocyte activation during in vivo fertilization in golden hamstersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0105-6263&volume=22&spage=68&epage=75&date=1999&atitle=Effects+of+male+accessory+sex+glands+on+sperm+decondensation+and+oocyte+activation+during+in+vivo+fertilization+in+golden+hamstersen_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-2605.1999.00146.xen_HK
dc.identifier.pmid10194637-
dc.identifier.scopuseid_2-s2.0-0033013468en_HK
dc.identifier.hkuros39949en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033013468&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue2en_HK
dc.identifier.spage68en_HK
dc.identifier.epage76en_HK
dc.identifier.isiWOS:000079369500002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYing, Y=36889049700en_HK
dc.identifier.scopusauthoridCheung, MPL=16749051200en_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats