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Conference Paper: Potential neuroprotective agent from botanical extract: An experience of using Verbena officinalis against β-amyloid peptide neurotoxicity

TitlePotential neuroprotective agent from botanical extract: An experience of using Verbena officinalis against β-amyloid peptide neurotoxicity
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 146, abstract no. P-10/25 How to Cite?
AbstractIncreasing lines of evidence have demonstrated the potential of using botanical extract to prevent neurodegenerative diseases. Verbena officinalis Linn. (Verbenaceae) is a perennial plant which has been used as herbal medicine or health supplement in both Western and Eastern countries for centuries. It has been used to treat acute dysentery, enteritis, amenorrhea and depression. In addition, aqueous extract of V. officinalis has been shown to exhibit sedative effects in an animal model. Since it has a wide array of biological effects, we hypothesized that V. officinalis can exert cytoprotective effects in central nervous system. Lactate dehydrogenase (LDH) assay was used as index of cytotoxicity for various toxins/stresses. Prior to the exposure of -amyloid (A ) peptide and reducing agent dithiothreitol, treatment of aqueous extracts of V. officinalis significantly attenuated their toxicity determined by LDH assay. As extracellular accumulation of A peptide has been considered to be an important pathological factor in Alz heimer’s disease (AD), we have further explored the neuroprotection of V. officinalis aqueous extracts against A . Treatment of V. officinalis aqueous extracts attenuated A -triggered DEVDand VDVAD-cleavage activities in dose-dependent manner. Furthermore, V. officinalis attenuated A -triggered apoptosis conDownlofirmed by DAPI staining. According to our previous findings, A peptide can activate the stress kinases, including double-stranded RNA-dependent protein kinase (PKR) and c-Jun N-terminal kinase (JNK). Our present results showed that V. officinalis attenuated the phosphorylation of both PKR and JNK in A -treated neurons by Western blot analysis. Taken together, we have proved our hypothesis by showing the novel neuroprotective effects of V. officinalis. As V. officinalis has long been used for many years to be a folk medicine, our study may provide a potential lead as a neuroprotective agent against neuronal loss in AD. Acknowledgement: S.W.L. and M.S.Y. are supported by postgraduate studentship from The University of Hong Kong. The study is partially supported by HKU Seed Funding for Basic Research (2004 & 2005) and HKU University Strategic Theme Research on Healthy Aging to R.C.C.C.
Persistent Identifierhttp://hdl.handle.net/10722/67815
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763

 

DC FieldValueLanguage
dc.contributor.authorLai, SWen_HK
dc.contributor.authorYu, MSen_HK
dc.contributor.authorYuen, WHen_HK
dc.contributor.authorZee, SYen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorChang, RCCen_HK
dc.date.accessioned2010-09-06T05:58:30Z-
dc.date.available2010-09-06T05:58:30Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 146, abstract no. P-10/25en_HK
dc.identifier.issn1424-862Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67815-
dc.description.abstractIncreasing lines of evidence have demonstrated the potential of using botanical extract to prevent neurodegenerative diseases. Verbena officinalis Linn. (Verbenaceae) is a perennial plant which has been used as herbal medicine or health supplement in both Western and Eastern countries for centuries. It has been used to treat acute dysentery, enteritis, amenorrhea and depression. In addition, aqueous extract of V. officinalis has been shown to exhibit sedative effects in an animal model. Since it has a wide array of biological effects, we hypothesized that V. officinalis can exert cytoprotective effects in central nervous system. Lactate dehydrogenase (LDH) assay was used as index of cytotoxicity for various toxins/stresses. Prior to the exposure of -amyloid (A ) peptide and reducing agent dithiothreitol, treatment of aqueous extracts of V. officinalis significantly attenuated their toxicity determined by LDH assay. As extracellular accumulation of A peptide has been considered to be an important pathological factor in Alz heimer’s disease (AD), we have further explored the neuroprotection of V. officinalis aqueous extracts against A . Treatment of V. officinalis aqueous extracts attenuated A -triggered DEVDand VDVAD-cleavage activities in dose-dependent manner. Furthermore, V. officinalis attenuated A -triggered apoptosis conDownlofirmed by DAPI staining. According to our previous findings, A peptide can activate the stress kinases, including double-stranded RNA-dependent protein kinase (PKR) and c-Jun N-terminal kinase (JNK). Our present results showed that V. officinalis attenuated the phosphorylation of both PKR and JNK in A -treated neurons by Western blot analysis. Taken together, we have proved our hypothesis by showing the novel neuroprotective effects of V. officinalis. As V. officinalis has long been used for many years to be a folk medicine, our study may provide a potential lead as a neuroprotective agent against neuronal loss in AD. Acknowledgement: S.W.L. and M.S.Y. are supported by postgraduate studentship from The University of Hong Kong. The study is partially supported by HKU Seed Funding for Basic Research (2004 & 2005) and HKU University Strategic Theme Research on Healthy Aging to R.C.C.C.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofNeurosignals-
dc.rightsNeurosignals. Copyright © S Karger AG.en_HK
dc.titlePotential neuroprotective agent from botanical extract: An experience of using Verbena officinalis against β-amyloid peptide neurotoxicityen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYuen, WH: yuenwh@HKUCC.hku.hken_HK
dc.identifier.emailZee, SY: botanya@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailChang, RCC: rccchang@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros124880en_HK
dc.identifier.hkuros112902-
dc.identifier.volume15-
dc.identifier.issue3-
dc.identifier.spage146, poster no. P-10/25-
dc.identifier.epage147, poster no. P-10/25-
dc.publisher.placeSwitzerland-
dc.description.otherThe 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 146-147, abstract no. P-10/25-

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