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Article: Expression of trkA, trkB, and trkC in injured and regenerating retinal ganglion cells of adult rats

TitleExpression of trkA, trkB, and trkC in injured and regenerating retinal ganglion cells of adult rats
Authors
Issue Date2002
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2002, v. 43 n. 6, p. 1954-1964 How to Cite?
AbstractPURPOSE. To investigate changes in percentage of tyrosine kinase (trk)A-, trkB-, and trkC-immunopositive ( +) retinal ganglion cells (RGCs) at various times after optic nerve (ON) axotomy; the proportion of RGCs regenerating axons into peripheral nerve (PN) grafts that are trkA +, trkB +, and trkC +; whether intravitreal PN-ON implants affect trk immunoreactivity; and the levels of trk mRNAs in ON-injured retinas. METHODS. The ON was transected intraorbitally. Proportions of trkA +, trkB +, and trkC + RGCs and levels of trk mRNAs were studied by using immunocytochemistry and Northern blot methods, respectively, in injured and RGC-regenerating retinas. RESULTS. In normal retinas, only small numbers of trkB + and trkC +, but not trkA +, RGCs were seen. The optic fiber layer was intensively immunolabeled with trkB. After ON injury, the proportions of trkA +, trkB +, and trkC + RGCs rapidly increased and reached their peaks by 3 to 5 days. During the next 3 weeks, the proportion of trkA + or trkB + RGCs gradually decreased, but the proportion of trkC + RGCs remained high. Intravitreal implants of PN+ON segments transiently but significantly suppressed injury-induced increases in all these trk + RGC proportions for approximately 5 days. In contrast, 3 days after ON injury, quantitative retinal expression of trkA mRNA, and to a lesser extent trkC mRNA, was downregulated, whereas trkB mRNA expression remained unaffected. Higher proportions of trkA + and trkB + RGCs and higher levels of all trk mRNAs were seen in regenerating RGCs and retinas, respectively. CONCLUSIONS. This study provides a kinetic analysis of expression of trk in RGCs and retinas after ON injury and during regeneration.
Persistent Identifierhttp://hdl.handle.net/10722/67810
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCui, Qen_HK
dc.contributor.authorTang, LSen_HK
dc.contributor.authorHu, Ben_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYip, HKen_HK
dc.date.accessioned2010-09-06T05:58:27Z-
dc.date.available2010-09-06T05:58:27Z-
dc.date.issued2002en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2002, v. 43 n. 6, p. 1954-1964en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67810-
dc.description.abstractPURPOSE. To investigate changes in percentage of tyrosine kinase (trk)A-, trkB-, and trkC-immunopositive ( +) retinal ganglion cells (RGCs) at various times after optic nerve (ON) axotomy; the proportion of RGCs regenerating axons into peripheral nerve (PN) grafts that are trkA +, trkB +, and trkC +; whether intravitreal PN-ON implants affect trk immunoreactivity; and the levels of trk mRNAs in ON-injured retinas. METHODS. The ON was transected intraorbitally. Proportions of trkA +, trkB +, and trkC + RGCs and levels of trk mRNAs were studied by using immunocytochemistry and Northern blot methods, respectively, in injured and RGC-regenerating retinas. RESULTS. In normal retinas, only small numbers of trkB + and trkC +, but not trkA +, RGCs were seen. The optic fiber layer was intensively immunolabeled with trkB. After ON injury, the proportions of trkA +, trkB +, and trkC + RGCs rapidly increased and reached their peaks by 3 to 5 days. During the next 3 weeks, the proportion of trkA + or trkB + RGCs gradually decreased, but the proportion of trkC + RGCs remained high. Intravitreal implants of PN+ON segments transiently but significantly suppressed injury-induced increases in all these trk + RGC proportions for approximately 5 days. In contrast, 3 days after ON injury, quantitative retinal expression of trkA mRNA, and to a lesser extent trkC mRNA, was downregulated, whereas trkB mRNA expression remained unaffected. Higher proportions of trkA + and trkB + RGCs and higher levels of all trk mRNAs were seen in regenerating RGCs and retinas, respectively. CONCLUSIONS. This study provides a kinetic analysis of expression of trk in RGCs and retinas after ON injury and during regeneration.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAxotomyen_HK
dc.subject.meshBlotting, Northernen_HK
dc.subject.meshCell Survivalen_HK
dc.subject.meshDNA Probesen_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshFluorescent Antibody Technique, Indirecten_HK
dc.subject.meshNerve Regeneration - physiologyen_HK
dc.subject.meshOptic Nerve - physiologyen_HK
dc.subject.meshOptic Nerve Injuries - enzymologyen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReceptor, trkA - genetics - metabolismen_HK
dc.subject.meshReceptor, trkB - genetics - metabolismen_HK
dc.subject.meshReceptor, trkC - genetics - metabolismen_HK
dc.subject.meshRetina - metabolismen_HK
dc.subject.meshRetinal Ganglion Cells - enzymologyen_HK
dc.subject.meshTime Factorsen_HK
dc.titleExpression of trkA, trkB, and trkC in injured and regenerating retinal ganglion cells of adult ratsen_HK
dc.typeArticleen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid12037005-
dc.identifier.scopuseid_2-s2.0-0036271504en_HK
dc.identifier.hkuros67575en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036271504&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1954en_HK
dc.identifier.epage1964en_HK
dc.identifier.isiWOS:000175927800040-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCui, Q=7103080164en_HK
dc.identifier.scopusauthoridTang, LS=7402081562en_HK
dc.identifier.scopusauthoridHu, B=35733928400en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK

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