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Article: Evidence of increased Id-1 expression and its role in cell proliferation in nasopharyngeal carcinoma cells

TitleEvidence of increased Id-1 expression and its role in cell proliferation in nasopharyngeal carcinoma cells
Authors
KeywordsId-1
Nasopharyngeal carcinoma cells
Proliferation
Issue Date2002
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/
Citation
Molecular Carcinogenesis, 2002, v. 35 n. 1, p. 42-49 How to Cite?
AbstractInhibitor of differentiation or DNA binding (Id-1), a helix-loop-helix transcription factor, has recently been shown to inactivate the retinoblastoma (RB)/p16INK4a pathway through down-regulation of p16INK4a and increasing phosphorylation of RB in certain cell types. Nasopharyngeal carcinoma (NPC) is a common cancer in Hong Kong, and inactivation of the tumor suppressor RB at transcription level is a rare event in NPC. The objective of this study was to investigate the role of Id-1 in NPC cell proliferation and its expression in NPC samples. An NPC cell line, CNE1, was transfected with a retroviral vector containing a full-length Id-1 cDNA, and six stable transfectant clones were isolated with differential Id-1 expression levels. The effect of ectopic Id-1 expression on serum-independent cell growth, cell-cycle distribution, and expression of proteins associated with RB pathway was studied. The Id-1 expression in five NPC samples was also investigated using immunohistochemistry. Ectopic Id-1 expression in CNE1 cells resulted in an increase in serum-independent cell growth, percentage of cells in S phase, and phosphorylation of RB and cyclin-dependent kinase 2 proteins. In addition, immunohistochemical studies on NPC samples showed that expression of Id-1 was present in NPC cells but absent in normal tissues. This study demonstrates that Id-1 plays an important role in cell proliferation in NPC cells, and our results provide evidence for the first time of the significance of Id-1 expression in NPC cells and suggest a possible role of Id-1 expression in the inactivation of RB and development of NPC. © 2002 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/67794
ISSN
2015 Impact Factor: 4.722
2015 SCImago Journal Rankings: 1.393
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_HK
dc.contributor.authorXu, Ken_HK
dc.contributor.authorLing, MTen_HK
dc.contributor.authorWong, YCen_HK
dc.contributor.authorFeng, HCen_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorTsao, SWen_HK
dc.date.accessioned2010-09-06T05:58:18Z-
dc.date.available2010-09-06T05:58:18Z-
dc.date.issued2002en_HK
dc.identifier.citationMolecular Carcinogenesis, 2002, v. 35 n. 1, p. 42-49en_HK
dc.identifier.issn0899-1987en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67794-
dc.description.abstractInhibitor of differentiation or DNA binding (Id-1), a helix-loop-helix transcription factor, has recently been shown to inactivate the retinoblastoma (RB)/p16INK4a pathway through down-regulation of p16INK4a and increasing phosphorylation of RB in certain cell types. Nasopharyngeal carcinoma (NPC) is a common cancer in Hong Kong, and inactivation of the tumor suppressor RB at transcription level is a rare event in NPC. The objective of this study was to investigate the role of Id-1 in NPC cell proliferation and its expression in NPC samples. An NPC cell line, CNE1, was transfected with a retroviral vector containing a full-length Id-1 cDNA, and six stable transfectant clones were isolated with differential Id-1 expression levels. The effect of ectopic Id-1 expression on serum-independent cell growth, cell-cycle distribution, and expression of proteins associated with RB pathway was studied. The Id-1 expression in five NPC samples was also investigated using immunohistochemistry. Ectopic Id-1 expression in CNE1 cells resulted in an increase in serum-independent cell growth, percentage of cells in S phase, and phosphorylation of RB and cyclin-dependent kinase 2 proteins. In addition, immunohistochemical studies on NPC samples showed that expression of Id-1 was present in NPC cells but absent in normal tissues. This study demonstrates that Id-1 plays an important role in cell proliferation in NPC cells, and our results provide evidence for the first time of the significance of Id-1 expression in NPC cells and suggest a possible role of Id-1 expression in the inactivation of RB and development of NPC. © 2002 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/en_HK
dc.relation.ispartofMolecular Carcinogenesisen_HK
dc.rightsMolecular Carcinogenesis. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectId-1en_HK
dc.subjectNasopharyngeal carcinoma cellsen_HK
dc.subjectProliferationen_HK
dc.subject.meshCDC2-CDC28 Kinasesen_HK
dc.subject.meshCarcinoma - metabolism - pathologyen_HK
dc.subject.meshCarrier Proteins - metabolismen_HK
dc.subject.meshCell Cycleen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshCulture Media, Serum-Freeen_HK
dc.subject.meshCyclin-Dependent Kinase 2en_HK
dc.subject.meshCyclin-Dependent Kinase 4en_HK
dc.subject.meshCyclin-Dependent Kinase Inhibitor p27en_HK
dc.subject.meshCyclin-Dependent Kinases - metabolismen_HK
dc.subject.meshDNA-Binding Proteins - genetics - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInhibitor of Differentiation Protein 1en_HK
dc.subject.meshIntracellular Signaling Peptides and Proteinsen_HK
dc.subject.meshNasopharyngeal Neoplasms - metabolism - pathologyen_HK
dc.subject.meshProtein-Serine-Threonine Kinases - metabolismen_HK
dc.subject.meshProto-Oncogene Proteinsen_HK
dc.subject.meshReference Valuesen_HK
dc.subject.meshRepressor Proteinsen_HK
dc.subject.meshRetinoblastoma Proteinen_HK
dc.subject.meshTranscription Factors - genetics - metabolismen_HK
dc.subject.meshTransfectionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleEvidence of increased Id-1 expression and its role in cell proliferation in nasopharyngeal carcinoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0899-1987&volume=35&spage=42&epage=49&date=2002&atitle=Evidence+of+increased+Id-1+expression+and+its+role+in+cell+proliferation+in+nasopharyngeal+carcinoma+cellsen_HK
dc.identifier.emailLing, MT:patling@hkucc.hku.hken_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.emailNicholls, J:nicholls@pathology.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityLing, MT=rp00449en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mc.10072en_HK
dc.identifier.pmid12203366en_HK
dc.identifier.scopuseid_2-s2.0-0036735348en_HK
dc.identifier.hkuros74876en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036735348&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue1en_HK
dc.identifier.spage42en_HK
dc.identifier.epage49en_HK
dc.identifier.isiWOS:000177821700006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, X=7501854829en_HK
dc.identifier.scopusauthoridXu, K=7403282051en_HK
dc.identifier.scopusauthoridLing, MT=7102229780en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK
dc.identifier.scopusauthoridFeng, HC=7401736336en_HK
dc.identifier.scopusauthoridNicholls, J=7201463077en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK

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