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Article: A morphometric study of optic axons regenerated in a sciatic nerve graft of adult rats

TitleA morphometric study of optic axons regenerated in a sciatic nerve graft of adult rats
Authors
KeywordsAxon-glial interaction
g-ratio
Microtubules
Neurofilaments
Optic nerve
Regenerated optic axons
Schwann cells
Issue Date2000
PublisherIOS Press. The Journal's web site is located at http://www.iospress.nl/html/09226028.php
Citation
Restorative Neurology And Neuroscience, 2000, v. 16 n. 2, p. 105-116 How to Cite?
AbstractPurpose: In the present study we have morphometrically examined a regeneration model in which axons normally residing in CNS have regrown and are interacting with Schwann cells from the PNS. This study will not only provide morphometric data on regenerated optic fibers but also shed light on possible factors in determining the fiber morphometry. Methods: The optic nerves of rats aged 6 weeks were cut intra-orbitally and replaced with a autologous sciatic nerve. After a survival period of 9 months, the graft or 'regenerated' nerves containing the regenerated optic axons and Schwann cells were processed for morphometric measurements. Results: The mean myelinated axon diameter of regenerated nerve (1.8 ±0.2 μm) was significantly (P < 0.05) greater than that of the optic nerve (0.9 ± 0.03 μm). However, unmyelinated regenerated optic axons had a smaller mean axon diameter (0.49 ± 0.04 μm) than normal myelinated optic axons. This may suggest that myelinating glial cells exert an influence on axon caliber and Schwann cells seem to have greater effect than oligodendrocytes. The mean g-ratio showing the relative myelin sheath thickness was found to be the highest in the optic nerve (0.78 ± 0.003), least in the sciatic nerve (0.6 ± 0.009) and intermediate in the regenerated nerve (0.68 ± 0.01). The results indicated that Schwann cells myelinating the regenerated optic axons have produced a thinner myelin sheath. Intra-axonally, no significant difference was detected in the number of axonal microtubules and neurofilaments between the regenerated and optic nerves. Therefore the disposition of microtubules and neurofilaments into axon may be intrinsically determined. Conclusions: In this study, we have identified some of the extrinsic and intrinsic factors in determining the fiber morphometry of the regenerated nerve. The axon-size and myelination by glial cells were determined through the external axon-glial interactions, whereas the number of axonal microtubules and neurofilaments were intrinsically determined.
Persistent Identifierhttp://hdl.handle.net/10722/67779
ISSN
2015 Impact Factor: 2.661
2015 SCImago Journal Rankings: 1.181

 

DC FieldValueLanguage
dc.contributor.authorWai Kei Chauen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorTay, Den_HK
dc.contributor.authorDockery, Pen_HK
dc.date.accessioned2010-09-06T05:58:10Z-
dc.date.available2010-09-06T05:58:10Z-
dc.date.issued2000en_HK
dc.identifier.citationRestorative Neurology And Neuroscience, 2000, v. 16 n. 2, p. 105-116en_HK
dc.identifier.issn0922-6028en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67779-
dc.description.abstractPurpose: In the present study we have morphometrically examined a regeneration model in which axons normally residing in CNS have regrown and are interacting with Schwann cells from the PNS. This study will not only provide morphometric data on regenerated optic fibers but also shed light on possible factors in determining the fiber morphometry. Methods: The optic nerves of rats aged 6 weeks were cut intra-orbitally and replaced with a autologous sciatic nerve. After a survival period of 9 months, the graft or 'regenerated' nerves containing the regenerated optic axons and Schwann cells were processed for morphometric measurements. Results: The mean myelinated axon diameter of regenerated nerve (1.8 ±0.2 μm) was significantly (P < 0.05) greater than that of the optic nerve (0.9 ± 0.03 μm). However, unmyelinated regenerated optic axons had a smaller mean axon diameter (0.49 ± 0.04 μm) than normal myelinated optic axons. This may suggest that myelinating glial cells exert an influence on axon caliber and Schwann cells seem to have greater effect than oligodendrocytes. The mean g-ratio showing the relative myelin sheath thickness was found to be the highest in the optic nerve (0.78 ± 0.003), least in the sciatic nerve (0.6 ± 0.009) and intermediate in the regenerated nerve (0.68 ± 0.01). The results indicated that Schwann cells myelinating the regenerated optic axons have produced a thinner myelin sheath. Intra-axonally, no significant difference was detected in the number of axonal microtubules and neurofilaments between the regenerated and optic nerves. Therefore the disposition of microtubules and neurofilaments into axon may be intrinsically determined. Conclusions: In this study, we have identified some of the extrinsic and intrinsic factors in determining the fiber morphometry of the regenerated nerve. The axon-size and myelination by glial cells were determined through the external axon-glial interactions, whereas the number of axonal microtubules and neurofilaments were intrinsically determined.en_HK
dc.languageengen_HK
dc.publisherIOS Press. The Journal's web site is located at http://www.iospress.nl/html/09226028.phpen_HK
dc.relation.ispartofRestorative Neurology and Neuroscienceen_HK
dc.rightsRestorative Neurology and Neuroscience. Copyright © IOS Press.en_HK
dc.subjectAxon-glial interactionen_HK
dc.subjectg-ratioen_HK
dc.subjectMicrotubulesen_HK
dc.subjectNeurofilamentsen_HK
dc.subjectOptic nerveen_HK
dc.subjectRegenerated optic axonsen_HK
dc.subjectSchwann cellsen_HK
dc.titleA morphometric study of optic axons regenerated in a sciatic nerve graft of adult ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0922-6028&volume=16&spage=105&epage=116&date=2000&atitle=A+morphometric+study+of+optic+axons+regenerated+in+a+sciatic+nerve+graft+of+adult+ratsen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailTay, D:dkctay@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityTay, D=rp00336en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12671213-
dc.identifier.scopuseid_2-s2.0-0034060215en_HK
dc.identifier.hkuros48572en_HK
dc.identifier.volume16en_HK
dc.identifier.issue2en_HK
dc.identifier.spage105en_HK
dc.identifier.epage116en_HK
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWai Kei Chau=7409775360en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridTay, D=7006796825en_HK
dc.identifier.scopusauthoridDockery, P=7004016702en_HK

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