File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Ciliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamsters

TitleCiliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamsters
Authors
KeywordsAxonal regeneration
GAP-43
Optic nerve transection
Peripheral nerve transplant
Sprouting
Vitreous
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 1999, v. 94 n. 2, p. 623-628 How to Cite?
AbstractCiliary neurotrophic factor has recently been shown to promote the axonal regrowth of retinal ganglion cells into a peripheral nerve graft following an intracranial transection of the optic nerve (~7mm from the optic disc). It is unclear whether the enhancement of axonal regrowth by ciliary neurotrophic factor application correlates with the enhancement of survival of retinal ganglion cells and/or the up-regulation of expression of growth-associated protein-43 messenger RNA in retinas. The present study evaluated the regenerative capacity of retinal ganglion cells following intraorbital transection of the optic nerve (~1.5mm from the optic disc) and the attachment of a peripheral nerve to the ocular stump of the optic nerve. In addition, we have determined the survival of retinal ganglion cells and the expression of growth-associated protein-43 messenger RNA in ciliary neurotrophic factor-treated retinas following optic nerve transection. The results showed that in the ciliary neurotrophic factor-treated retinas, the number of retinal ganglion cells which had regrown axons into a peripheral nerve is about four times more than the control. In the axotomized retinas, ciliary neurotrophic factor initiated sprouting of axon-like processes at 14 and 28 days post-axotomy and up-regulated the expression level of growth-associated protein-43 messenger RNA at 7,14 and 28 days post-axotomy. However, ciliary neurotrophic factor did not prevent the death of axotomized retinal ganglion cells.We suggest that one possible mechanism for the axonal regeneration of axotomized retinal ganglion cells by ciliary neurotrophic factor could be mediated by the up-regulation of growth-associated protein-43 gene expression and not by increasing the pool of surviving retinal ganglion cells after axotomy. Copyright (C) 1999 IBRO.
Persistent Identifierhttp://hdl.handle.net/10722/67773
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCho, KSen_HK
dc.contributor.authorChan, PMen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorChung, SKen_HK
dc.date.accessioned2010-09-06T05:58:07Z-
dc.date.available2010-09-06T05:58:07Z-
dc.date.issued1999en_HK
dc.identifier.citationNeuroscience, 1999, v. 94 n. 2, p. 623-628en_HK
dc.identifier.issn0306-4522en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67773-
dc.description.abstractCiliary neurotrophic factor has recently been shown to promote the axonal regrowth of retinal ganglion cells into a peripheral nerve graft following an intracranial transection of the optic nerve (~7mm from the optic disc). It is unclear whether the enhancement of axonal regrowth by ciliary neurotrophic factor application correlates with the enhancement of survival of retinal ganglion cells and/or the up-regulation of expression of growth-associated protein-43 messenger RNA in retinas. The present study evaluated the regenerative capacity of retinal ganglion cells following intraorbital transection of the optic nerve (~1.5mm from the optic disc) and the attachment of a peripheral nerve to the ocular stump of the optic nerve. In addition, we have determined the survival of retinal ganglion cells and the expression of growth-associated protein-43 messenger RNA in ciliary neurotrophic factor-treated retinas following optic nerve transection. The results showed that in the ciliary neurotrophic factor-treated retinas, the number of retinal ganglion cells which had regrown axons into a peripheral nerve is about four times more than the control. In the axotomized retinas, ciliary neurotrophic factor initiated sprouting of axon-like processes at 14 and 28 days post-axotomy and up-regulated the expression level of growth-associated protein-43 messenger RNA at 7,14 and 28 days post-axotomy. However, ciliary neurotrophic factor did not prevent the death of axotomized retinal ganglion cells.We suggest that one possible mechanism for the axonal regeneration of axotomized retinal ganglion cells by ciliary neurotrophic factor could be mediated by the up-regulation of growth-associated protein-43 gene expression and not by increasing the pool of surviving retinal ganglion cells after axotomy. Copyright (C) 1999 IBRO.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_HK
dc.relation.ispartofNeuroscienceen_HK
dc.rightsNeuroscience. Copyright © Elsevier BV.en_HK
dc.subjectAxonal regenerationen_HK
dc.subjectGAP-43en_HK
dc.subjectOptic nerve transectionen_HK
dc.subjectPeripheral nerve transplanten_HK
dc.subjectSproutingen_HK
dc.subjectVitreousen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAxotomyen_HK
dc.subject.meshCell Division - drug effectsen_HK
dc.subject.meshCell Survivalen_HK
dc.subject.meshCiliary Neurotrophic Factor - administration & dosage - pharmacologyen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshGAP-43 Protein - analysisen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMesocricetusen_HK
dc.subject.meshMicroinjectionsen_HK
dc.subject.meshNerve Regeneration - drug effects - physiologyen_HK
dc.subject.meshOptic Nerve - physiologyen_HK
dc.subject.meshRetinal Ganglion Cells - cytology - drug effects - physiologyen_HK
dc.subject.meshTime Factorsen_HK
dc.titleCiliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamstersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0306-4522&volume=94&issue=2&spage=623&epage=628&date=1999&atitle=Ciliary+neurotrophic+factor+promotes+the+regrowth+capacity+but+not+the+survival+of+intraorbitally+axotomized+retinal+ganglion+cells+in+adult+hamstersen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.emailChung, SK:skchung@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0306-4522(99)00320-6en_HK
dc.identifier.pmid10579222-
dc.identifier.scopuseid_2-s2.0-0032849704en_HK
dc.identifier.hkuros47669en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032849704&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume94en_HK
dc.identifier.issue2en_HK
dc.identifier.spage623en_HK
dc.identifier.epage628en_HK
dc.identifier.isiWOS:000083135600025-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridCho, KS=7403956958en_HK
dc.identifier.scopusauthoridChan, PM=12788250500en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats