Article: Ciliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamsters

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TitleCiliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamsters
AuthorsCho, KS1
Chan, PM1
So, KF1
Yip, HK1
Chung, SK1
KeywordsAxonal regeneration
GAP-43
Optic nerve transection
Peripheral nerve transplant
Sprouting
Vitreous
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
CitationNeuroscience, 1999, v. 94 n. 2, p. 623-628 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0306-4522(99)00320-6
AbstractCiliary neurotrophic factor has recently been shown to promote the axonal regrowth of retinal ganglion cells into a peripheral nerve graft following an intracranial transection of the optic nerve (~7mm from the optic disc). It is unclear whether the enhancement of axonal regrowth by ciliary neurotrophic factor application correlates with the enhancement of survival of retinal ganglion cells and/or the up-regulation of expression of growth-associated protein-43 messenger RNA in retinas. The present study evaluated the regenerative capacity of retinal ganglion cells following intraorbital transection of the optic nerve (~1.5mm from the optic disc) and the attachment of a peripheral nerve to the ocular stump of the optic nerve. In addition, we have determined the survival of retinal ganglion cells and the expression of growth-associated protein-43 messenger RNA in ciliary neurotrophic factor-treated retinas following optic nerve transection. The results showed that in the ciliary neurotrophic factor-treated retinas, the number of retinal ganglion cells which had regrown axons into a peripheral nerve is about four times more than the control. In the axotomized retinas, ciliary neurotrophic factor initiated sprouting of axon-like processes at 14 and 28 days post-axotomy and up-regulated the expression level of growth-associated protein-43 messenger RNA at 7,14 and 28 days post-axotomy. However, ciliary neurotrophic factor did not prevent the death of axotomized retinal ganglion cells.We suggest that one possible mechanism for the axonal regeneration of axotomized retinal ganglion cells by ciliary neurotrophic factor could be mediated by the up-regulation of growth-associated protein-43 gene expression and not by increasing the pool of surviving retinal ganglion cells after axotomy. Copyright (C) 1999 IBRO.
ISSN0306-4522
2011 Impact Factor: 3.38
2011 SCImago Journal Rankings: 0.284
DOIhttp://dx.doi.org/10.1016/S0306-4522(99)00320-6
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorCho, KS
dc.contributor.authorChan, PM
dc.contributor.authorSo, KF
dc.contributor.authorYip, HK
dc.contributor.authorChung, SK
dc.date.accessioned2010-09-06T05:58:07Z
dc.date.available2010-09-06T05:58:07Z
dc.date.issued1999
dc.description.abstractCiliary neurotrophic factor has recently been shown to promote the axonal regrowth of retinal ganglion cells into a peripheral nerve graft following an intracranial transection of the optic nerve (~7mm from the optic disc). It is unclear whether the enhancement of axonal regrowth by ciliary neurotrophic factor application correlates with the enhancement of survival of retinal ganglion cells and/or the up-regulation of expression of growth-associated protein-43 messenger RNA in retinas. The present study evaluated the regenerative capacity of retinal ganglion cells following intraorbital transection of the optic nerve (~1.5mm from the optic disc) and the attachment of a peripheral nerve to the ocular stump of the optic nerve. In addition, we have determined the survival of retinal ganglion cells and the expression of growth-associated protein-43 messenger RNA in ciliary neurotrophic factor-treated retinas following optic nerve transection. The results showed that in the ciliary neurotrophic factor-treated retinas, the number of retinal ganglion cells which had regrown axons into a peripheral nerve is about four times more than the control. In the axotomized retinas, ciliary neurotrophic factor initiated sprouting of axon-like processes at 14 and 28 days post-axotomy and up-regulated the expression level of growth-associated protein-43 messenger RNA at 7,14 and 28 days post-axotomy. However, ciliary neurotrophic factor did not prevent the death of axotomized retinal ganglion cells.We suggest that one possible mechanism for the axonal regeneration of axotomized retinal ganglion cells by ciliary neurotrophic factor could be mediated by the up-regulation of growth-associated protein-43 gene expression and not by increasing the pool of surviving retinal ganglion cells after axotomy. Copyright (C) 1999 IBRO.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationNeuroscience, 1999, v. 94 n. 2, p. 623-628 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0306-4522(99)00320-6
dc.identifier.doihttp://dx.doi.org/10.1016/S0306-4522(99)00320-6
dc.identifier.epage628
dc.identifier.hkuros47669
dc.identifier.isiWOS:000083135600025
dc.identifier.issn0306-4522
2011 Impact Factor: 3.38
2011 SCImago Journal Rankings: 0.284
dc.identifier.issue2
dc.identifier.openurl
dc.identifier.pmid10579222
dc.identifier.scopuseid_2-s2.0-0032849704
dc.identifier.spage623
dc.identifier.urihttp://hdl.handle.net/10722/67773
dc.identifier.volume94
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
dc.publisher.placeNetherlands
dc.relation.ispartofNeuroscience
dc.relation.referencesReferences in Scopus
dc.rightsNeuroscience. Copyright © Elsevier BV.
dc.subject.meshAnimals
dc.subject.meshAxotomy
dc.subject.meshCell Division - drug effects
dc.subject.meshCell Survival
dc.subject.meshCiliary Neurotrophic Factor - administration & dosage - pharmacology
dc.subject.meshCricetinae
dc.subject.meshGAP-43 Protein - analysis
dc.subject.meshMale
dc.subject.meshMesocricetus
dc.subject.meshMicroinjections
dc.subject.meshNerve Regeneration - drug effects - physiology
dc.subject.meshOptic Nerve - physiology
dc.subject.meshRetinal Ganglion Cells - cytology - drug effects - physiology
dc.subject.meshTime Factors
dc.subjectAxonal regeneration
dc.subjectGAP-43
dc.subjectOptic nerve transection
dc.subjectPeripheral nerve transplant
dc.subjectSprouting
dc.subjectVitreous
dc.titleCiliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamsters
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong