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Article: Ciliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamsters
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TitleCiliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamsters
 
AuthorsCho, KS1
Chan, PM1
So, KF1
Yip, HK1
Chung, SK1
 
KeywordsAxonal regeneration
GAP-43
Optic nerve transection
Peripheral nerve transplant
Sprouting
Vitreous
 
Issue Date1999
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
 
CitationNeuroscience, 1999, v. 94 n. 2, p. 623-628 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0306-4522(99)00320-6
 
AbstractCiliary neurotrophic factor has recently been shown to promote the axonal regrowth of retinal ganglion cells into a peripheral nerve graft following an intracranial transection of the optic nerve (~7mm from the optic disc). It is unclear whether the enhancement of axonal regrowth by ciliary neurotrophic factor application correlates with the enhancement of survival of retinal ganglion cells and/or the up-regulation of expression of growth-associated protein-43 messenger RNA in retinas. The present study evaluated the regenerative capacity of retinal ganglion cells following intraorbital transection of the optic nerve (~1.5mm from the optic disc) and the attachment of a peripheral nerve to the ocular stump of the optic nerve. In addition, we have determined the survival of retinal ganglion cells and the expression of growth-associated protein-43 messenger RNA in ciliary neurotrophic factor-treated retinas following optic nerve transection. The results showed that in the ciliary neurotrophic factor-treated retinas, the number of retinal ganglion cells which had regrown axons into a peripheral nerve is about four times more than the control. In the axotomized retinas, ciliary neurotrophic factor initiated sprouting of axon-like processes at 14 and 28 days post-axotomy and up-regulated the expression level of growth-associated protein-43 messenger RNA at 7,14 and 28 days post-axotomy. However, ciliary neurotrophic factor did not prevent the death of axotomized retinal ganglion cells.We suggest that one possible mechanism for the axonal regeneration of axotomized retinal ganglion cells by ciliary neurotrophic factor could be mediated by the up-regulation of growth-associated protein-43 gene expression and not by increasing the pool of surviving retinal ganglion cells after axotomy. Copyright (C) 1999 IBRO.
 
ISSN0306-4522
2012 Impact Factor: 3.122
2012 SCImago Journal Rankings: 1.498
 
DOIhttp://dx.doi.org/10.1016/S0306-4522(99)00320-6
 
ISI Accession Number IDWOS:000083135600025
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCho, KS
 
dc.contributor.authorChan, PM
 
dc.contributor.authorSo, KF
 
dc.contributor.authorYip, HK
 
dc.contributor.authorChung, SK
 
dc.date.accessioned2010-09-06T05:58:07Z
 
dc.date.available2010-09-06T05:58:07Z
 
dc.date.issued1999
 
dc.description.abstractCiliary neurotrophic factor has recently been shown to promote the axonal regrowth of retinal ganglion cells into a peripheral nerve graft following an intracranial transection of the optic nerve (~7mm from the optic disc). It is unclear whether the enhancement of axonal regrowth by ciliary neurotrophic factor application correlates with the enhancement of survival of retinal ganglion cells and/or the up-regulation of expression of growth-associated protein-43 messenger RNA in retinas. The present study evaluated the regenerative capacity of retinal ganglion cells following intraorbital transection of the optic nerve (~1.5mm from the optic disc) and the attachment of a peripheral nerve to the ocular stump of the optic nerve. In addition, we have determined the survival of retinal ganglion cells and the expression of growth-associated protein-43 messenger RNA in ciliary neurotrophic factor-treated retinas following optic nerve transection. The results showed that in the ciliary neurotrophic factor-treated retinas, the number of retinal ganglion cells which had regrown axons into a peripheral nerve is about four times more than the control. In the axotomized retinas, ciliary neurotrophic factor initiated sprouting of axon-like processes at 14 and 28 days post-axotomy and up-regulated the expression level of growth-associated protein-43 messenger RNA at 7,14 and 28 days post-axotomy. However, ciliary neurotrophic factor did not prevent the death of axotomized retinal ganglion cells.We suggest that one possible mechanism for the axonal regeneration of axotomized retinal ganglion cells by ciliary neurotrophic factor could be mediated by the up-regulation of growth-associated protein-43 gene expression and not by increasing the pool of surviving retinal ganglion cells after axotomy. Copyright (C) 1999 IBRO.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationNeuroscience, 1999, v. 94 n. 2, p. 623-628 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0306-4522(99)00320-6
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0306-4522(99)00320-6
 
dc.identifier.epage628
 
dc.identifier.hkuros47669
 
dc.identifier.isiWOS:000083135600025
 
dc.identifier.issn0306-4522
2012 Impact Factor: 3.122
2012 SCImago Journal Rankings: 1.498
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid10579222
 
dc.identifier.scopuseid_2-s2.0-0032849704
 
dc.identifier.spage623
 
dc.identifier.urihttp://hdl.handle.net/10722/67773
 
dc.identifier.volume94
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofNeuroscience
 
dc.relation.referencesReferences in Scopus
 
dc.rightsNeuroscience. Copyright © Elsevier BV.
 
dc.subject.meshAnimals
 
dc.subject.meshAxotomy
 
dc.subject.meshCell Division - drug effects
 
dc.subject.meshCell Survival
 
dc.subject.meshCiliary Neurotrophic Factor - administration & dosage - pharmacology
 
dc.subject.meshCricetinae
 
dc.subject.meshGAP-43 Protein - analysis
 
dc.subject.meshMale
 
dc.subject.meshMesocricetus
 
dc.subject.meshMicroinjections
 
dc.subject.meshNerve Regeneration - drug effects - physiology
 
dc.subject.meshOptic Nerve - physiology
 
dc.subject.meshRetinal Ganglion Cells - cytology - drug effects - physiology
 
dc.subject.meshTime Factors
 
dc.subjectAxonal regeneration
 
dc.subjectGAP-43
 
dc.subjectOptic nerve transection
 
dc.subjectPeripheral nerve transplant
 
dc.subjectSprouting
 
dc.subjectVitreous
 
dc.titleCiliary neurotrophic factor promotes the regrowth capacity but not the survival of intraorbitally axotomized retinal ganglion cells in adult hamsters
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong