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Article: Neuroprotective effects of polysaccharides from wolfberry, the fruits of lycium barbarum, against homocysteine-induced toxicity in rat cortical neurons

TitleNeuroprotective effects of polysaccharides from wolfberry, the fruits of lycium barbarum, against homocysteine-induced toxicity in rat cortical neurons
Authors
KeywordsApoptosis
Homocysteine
Neuroprotection
Tau phosphorylation
Wolfberry
Issue Date2010
PublisherIOS Press. The Journal's web site is located at http://www.iospress.nl/html/13872877.php
Citation
Journal Of Alzheimer's Disease, 2010, v. 19 n. 3, p. 813-827 How to Cite?
AbstractPrevious clinical and epidemiological studies have suggested that elevated plasma homocysteine (Hcy) levels increased the risk of Alzheime's disease (AD). Although the underlying mechanisms of its toxicity are elusive, it has been shown that Hcy damages neurons by inducing apoptosis, DNA fragmentation, and tau hyperphosphorylation. Wolfberry (Lycium barbarum) is a fruit that is known for its eye-protective and anti-aging properties in Asian countries. Previous studies from our laboratory have demonstrated that polysaccharides derived from wolfberry (LBA) have the ability to protect neurons from amyloid-β (Aβ) peptide neurotoxicity. We hypothesize that the neuroprotective effects of wolfberry is not limited to Aβ and can also provide protection against other AD risk factors. In this study, we aim to elucidate the neuroprotective effects of wolfberry against Hcy-induced neuronal damage. Our data showed that LBA treatment significantly attenuated Hcy-induced neuronal cell death and apoptosis in primary cortical neurons as demonstrated by LDH and caspase-3 like activity assay. LBA also significantly reduced Hcy-induced tau phosphorylation at tau-1 (Ser198/199/202), pS396 (Ser396), and pS214 (Ser214) epitopes as well as cleavage of tau. At the same time, we also found that the phosphorylation level of p-GSK3β (Ser9/Tyr 216) remained unchanged among different treatment groups at all detected time points. LBA treatment suppressed elevation of both p-ERK and p-JNK. In summary, our data demonstrated that LBA exerted neuroprotective effects on cortical neurons exposed to Hcy. Therefore, LBA has the potential to be a diseasemodifying agent for the prevention of AD. © 2010 - IOS Press and the authors. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67738
ISSN
2015 Impact Factor: 3.92
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
Funding AgencyGrant Number
HKU Alzheimer's Disease Research Network
Azalea (1972) Endowment Fund
General Research Grant755206M
761609M
NSFC/RGCN HKU707/07M
Research Council
HKU Seed Funding for Basic Research200811159082
Department of Chemistry
University of Hong Kong
Funding Information:

The authors would like to thank Professor J. N. Fang for the help in extracting Wolfberry for LBA, and Michelle Huie for critical reading of our manuscript. This work is supported by the HKU Alzheimer's Disease Research Network, Azalea (1972) Endowment Fund, General Research Grant (755206M & 761609M) and NSFC/RGC Joint Research Scheme (N HKU707/07M) from Research Council and HKU Seed Funding for Basic Research (200811159082) to RCCC. WHY would like to thank for the support from the Department of Chemistry. YSH and MSY are supported by Postdoctoral Fellowship. MSY supported by a Postdoctoral Fellowship, The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorHo, YSen_HK
dc.contributor.authorYu, MSen_HK
dc.contributor.authorYang, XFen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYuen, WHen_HK
dc.contributor.authorChang, RCCen_HK
dc.date.accessioned2010-09-06T05:57:49Z-
dc.date.available2010-09-06T05:57:49Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Alzheimer's Disease, 2010, v. 19 n. 3, p. 813-827en_HK
dc.identifier.issn1387-2877en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67738-
dc.description.abstractPrevious clinical and epidemiological studies have suggested that elevated plasma homocysteine (Hcy) levels increased the risk of Alzheime's disease (AD). Although the underlying mechanisms of its toxicity are elusive, it has been shown that Hcy damages neurons by inducing apoptosis, DNA fragmentation, and tau hyperphosphorylation. Wolfberry (Lycium barbarum) is a fruit that is known for its eye-protective and anti-aging properties in Asian countries. Previous studies from our laboratory have demonstrated that polysaccharides derived from wolfberry (LBA) have the ability to protect neurons from amyloid-β (Aβ) peptide neurotoxicity. We hypothesize that the neuroprotective effects of wolfberry is not limited to Aβ and can also provide protection against other AD risk factors. In this study, we aim to elucidate the neuroprotective effects of wolfberry against Hcy-induced neuronal damage. Our data showed that LBA treatment significantly attenuated Hcy-induced neuronal cell death and apoptosis in primary cortical neurons as demonstrated by LDH and caspase-3 like activity assay. LBA also significantly reduced Hcy-induced tau phosphorylation at tau-1 (Ser198/199/202), pS396 (Ser396), and pS214 (Ser214) epitopes as well as cleavage of tau. At the same time, we also found that the phosphorylation level of p-GSK3β (Ser9/Tyr 216) remained unchanged among different treatment groups at all detected time points. LBA treatment suppressed elevation of both p-ERK and p-JNK. In summary, our data demonstrated that LBA exerted neuroprotective effects on cortical neurons exposed to Hcy. Therefore, LBA has the potential to be a diseasemodifying agent for the prevention of AD. © 2010 - IOS Press and the authors. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherIOS Press. The Journal's web site is located at http://www.iospress.nl/html/13872877.phpen_HK
dc.relation.ispartofJournal of Alzheimer's Diseaseen_HK
dc.subjectApoptosisen_HK
dc.subjectHomocysteineen_HK
dc.subjectNeuroprotectionen_HK
dc.subjectTau phosphorylationen_HK
dc.subjectWolfberryen_HK
dc.subject.meshAlzheimer Disease - metabolism - pathology - prevention and control-
dc.subject.meshCerebral Cortex - drug effects - metabolism - pathology-
dc.subject.meshDrugs, Chinese Herbal - administration and dosage - pharmacology-
dc.subject.meshHomocysteine - antagonists and inhibitors - toxicity-
dc.subject.meshNeuroprotective Agents - administration and dosage - pharmacology-
dc.titleNeuroprotective effects of polysaccharides from wolfberry, the fruits of lycium barbarum, against homocysteine-induced toxicity in rat cortical neuronsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1387-2877&volume=19&issue=3&spage=813&epage=827&date=2010&atitle=Neuroprotective+effects+of+polysaccharides+from+wolfberry,+the+fruits+of+Lycium+barbarum.+against+homocysteine-induced+toxicity+in+rat+cortical+neuronsen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3233/JAD-2010-1280en_HK
dc.identifier.pmid20157238-
dc.identifier.scopuseid_2-s2.0-77149149070en_HK
dc.identifier.hkuros169290en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77149149070&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue3en_HK
dc.identifier.spage813en_HK
dc.identifier.epage827en_HK
dc.identifier.isiWOS:000276617100004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHo, YS=14031513600en_HK
dc.identifier.scopusauthoridYu, MS=35346047600en_HK
dc.identifier.scopusauthoridYang, XF=23007172600en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridYuen, WH=7102761282en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK

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