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Article: Establishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes

TitleEstablishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes
Authors
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
Citation
Biochimica Et Biophysica Acta - Molecular Cell Research, 2002, v. 1590 n. 1-3, p. 150-158 How to Cite?
AbstractNasopharyngeal carcinoma (NPC) is a common cancer in Southeast Asia, especially in southern China. One of the most striking features of this disease is its close relationship with Epstein-Barr Virus (EBV). However, to date there is no direct study on the mechanisms involved in the role of EBV in the tumorigenesis of NPC, largely due to lack of an experimental model. Available hypotheses on the association between EBV and NPC are generated from non-nasopharyngeal epithelial cell systems such as human keratinocytes or mouse epithelial cells, which may not truly represent the biological properties of nasopharyngeal epithelial (NP) cells. In this study, we report the establishment of two immortalized NP cell lines, NP69SV40T and NP39E6/E7, using SV40T and HPV16E6/E7 oncogenes. We found that NP60SV40T and NP39E6/E7 cell lines not only maintained many characteristics of normal NP cells (i.e. keratin profile and responsive to TGFβ inhibition) but also highly responsive to one of the EBV encoded genes, LMP1. Comparative genome hybridization (CGH) analysis showed that these two cell lines contained multiple genetic alterations, some of which have been described in NPC. The immortalized NP cell lines are non-tumorigenic and exhibit anchorage-dependent growth. These cell lines may provide a possible cell model system for studying the mechanisms involved in the tumorigenesis of NPC. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67737
ISSN
2014 Impact Factor: 5.019
2014 SCImago Journal Rankings: 2.523
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorCheung, YCen_HK
dc.contributor.authorFeng, Hen_HK
dc.contributor.authorZheng, Zen_HK
dc.contributor.authorWong, Nen_HK
dc.contributor.authorYuen, PWen_HK
dc.contributor.authorLo, AKFen_HK
dc.contributor.authorWong, YCen_HK
dc.contributor.authorHuang, DPen_HK
dc.date.accessioned2010-09-06T05:57:48Z-
dc.date.available2010-09-06T05:57:48Z-
dc.date.issued2002en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Cell Research, 2002, v. 1590 n. 1-3, p. 150-158en_HK
dc.identifier.issn0167-4889en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67737-
dc.description.abstractNasopharyngeal carcinoma (NPC) is a common cancer in Southeast Asia, especially in southern China. One of the most striking features of this disease is its close relationship with Epstein-Barr Virus (EBV). However, to date there is no direct study on the mechanisms involved in the role of EBV in the tumorigenesis of NPC, largely due to lack of an experimental model. Available hypotheses on the association between EBV and NPC are generated from non-nasopharyngeal epithelial cell systems such as human keratinocytes or mouse epithelial cells, which may not truly represent the biological properties of nasopharyngeal epithelial (NP) cells. In this study, we report the establishment of two immortalized NP cell lines, NP69SV40T and NP39E6/E7, using SV40T and HPV16E6/E7 oncogenes. We found that NP60SV40T and NP39E6/E7 cell lines not only maintained many characteristics of normal NP cells (i.e. keratin profile and responsive to TGFβ inhibition) but also highly responsive to one of the EBV encoded genes, LMP1. Comparative genome hybridization (CGH) analysis showed that these two cell lines contained multiple genetic alterations, some of which have been described in NPC. The immortalized NP cell lines are non-tumorigenic and exhibit anchorage-dependent growth. These cell lines may provide a possible cell model system for studying the mechanisms involved in the tumorigenesis of NPC. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcren_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Cell Researchen_HK
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.en_HK
dc.subject.meshAntigens, Polyomavirus Transforming - geneticsen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshChromosomes, Human - geneticsen_HK
dc.subject.meshEnzyme Activationen_HK
dc.subject.meshEpithelial Cells - cytology - drug effects - metabolismen_HK
dc.subject.meshGenes, Viralen_HK
dc.subject.meshHerpesvirus 4, Human - pathogenicityen_HK
dc.subject.meshHumansen_HK
dc.subject.meshModels, Biologicalen_HK
dc.subject.meshNasopharyngeal Neoplasms - etiologyen_HK
dc.subject.meshNasopharynx - cytology - drug effects - metabolismen_HK
dc.subject.meshNucleic Acid Hybridizationen_HK
dc.subject.meshOncogene Proteins, Viral - geneticsen_HK
dc.subject.meshPapillomavirus E7 Proteinsen_HK
dc.subject.meshRepressor Proteinsen_HK
dc.subject.meshSimian virus 40 - geneticsen_HK
dc.subject.meshTelomerase - metabolismen_HK
dc.subject.meshTransforming Growth Factor beta - pharmacologyen_HK
dc.subject.meshTransforming Growth Factor beta1en_HK
dc.subject.meshViral Matrix Proteins - metabolismen_HK
dc.titleEstablishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3002&volume=1590&spage=150&epage=158&date=2002&atitle=Establishment+of+two+immortalized+nasopharyngeal+epithelial+cell+lines+using+SV40+large+T+and+HPV16E6/E7+viral+oncogenesen_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0167-4889(02)00208-2en_HK
dc.identifier.pmid12063178en_HK
dc.identifier.scopuseid_2-s2.0-0037067180en_HK
dc.identifier.hkuros69736en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037067180&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1590en_HK
dc.identifier.issue1-3en_HK
dc.identifier.spage150en_HK
dc.identifier.epage158en_HK
dc.identifier.isiWOS:000176518700015-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridWang, X=7501854829en_HK
dc.identifier.scopusauthoridLiu, Y=26643293600en_HK
dc.identifier.scopusauthoridCheung, YC=7202111087en_HK
dc.identifier.scopusauthoridFeng, H=7401736336en_HK
dc.identifier.scopusauthoridZheng, Z=36792437600en_HK
dc.identifier.scopusauthoridWong, N=7202836653en_HK
dc.identifier.scopusauthoridYuen, PW=7103124007en_HK
dc.identifier.scopusauthoridLo, AKF=7102780657en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK
dc.identifier.scopusauthoridHuang, DP=7403891486en_HK

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