Article: Establishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes

File Download Links for fulltext
(May Require Subscription)
Supplementary
  • Basic View
  • Metadata View
  • XML View
TitleEstablishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes
AuthorsTsao, SW1
Wang, X1
Liu, Y1
Cheung, YC1
Feng, H1
Zheng, Z1
Wong, N2
Yuen, PW1
Lo, AKF1
Wong, YC1
Huang, DP3
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
CitationBiochimica Et Biophysica Acta - Molecular Cell Research, 2002, v. 1590 n. 1-3, p. 150-158 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4889(02)00208-2
AbstractNasopharyngeal carcinoma (NPC) is a common cancer in Southeast Asia, especially in southern China. One of the most striking features of this disease is its close relationship with Epstein-Barr Virus (EBV). However, to date there is no direct study on the mechanisms involved in the role of EBV in the tumorigenesis of NPC, largely due to lack of an experimental model. Available hypotheses on the association between EBV and NPC are generated from non-nasopharyngeal epithelial cell systems such as human keratinocytes or mouse epithelial cells, which may not truly represent the biological properties of nasopharyngeal epithelial (NP) cells. In this study, we report the establishment of two immortalized NP cell lines, NP69SV40T and NP39E6/E7, using SV40T and HPV16E6/E7 oncogenes. We found that NP60SV40T and NP39E6/E7 cell lines not only maintained many characteristics of normal NP cells (i.e. keratin profile and responsive to TGFβ inhibition) but also highly responsive to one of the EBV encoded genes, LMP1. Comparative genome hybridization (CGH) analysis showed that these two cell lines contained multiple genetic alterations, some of which have been described in NPC. The immortalized NP cell lines are non-tumorigenic and exhibit anchorage-dependent growth. These cell lines may provide a possible cell model system for studying the mechanisms involved in the tumorigenesis of NPC. © 2002 Elsevier Science B.V. All rights reserved.
ISSN0167-4889
2011 Impact Factor: 5.538
2011 SCImago Journal Rankings: 0.707
DOIhttp://dx.doi.org/10.1016/S0167-4889(02)00208-2
ISI Accession Number IDWOS:000176518700015
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorTsao, SW
dc.contributor.authorWang, X
dc.contributor.authorLiu, Y
dc.contributor.authorCheung, YC
dc.contributor.authorFeng, H
dc.contributor.authorZheng, Z
dc.contributor.authorWong, N
dc.contributor.authorYuen, PW
dc.contributor.authorLo, AKF
dc.contributor.authorWong, YC
dc.contributor.authorHuang, DP
dc.date.accessioned2010-09-06T05:57:48Z
dc.date.available2010-09-06T05:57:48Z
dc.date.issued2002
dc.description.abstractNasopharyngeal carcinoma (NPC) is a common cancer in Southeast Asia, especially in southern China. One of the most striking features of this disease is its close relationship with Epstein-Barr Virus (EBV). However, to date there is no direct study on the mechanisms involved in the role of EBV in the tumorigenesis of NPC, largely due to lack of an experimental model. Available hypotheses on the association between EBV and NPC are generated from non-nasopharyngeal epithelial cell systems such as human keratinocytes or mouse epithelial cells, which may not truly represent the biological properties of nasopharyngeal epithelial (NP) cells. In this study, we report the establishment of two immortalized NP cell lines, NP69SV40T and NP39E6/E7, using SV40T and HPV16E6/E7 oncogenes. We found that NP60SV40T and NP39E6/E7 cell lines not only maintained many characteristics of normal NP cells (i.e. keratin profile and responsive to TGFβ inhibition) but also highly responsive to one of the EBV encoded genes, LMP1. Comparative genome hybridization (CGH) analysis showed that these two cell lines contained multiple genetic alterations, some of which have been described in NPC. The immortalized NP cell lines are non-tumorigenic and exhibit anchorage-dependent growth. These cell lines may provide a possible cell model system for studying the mechanisms involved in the tumorigenesis of NPC. © 2002 Elsevier Science B.V. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Cell Research, 2002, v. 1590 n. 1-3, p. 150-158 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4889(02)00208-2
dc.identifier.doihttp://dx.doi.org/10.1016/S0167-4889(02)00208-2
dc.identifier.epage158
dc.identifier.hkuros69736
dc.identifier.isiWOS:000176518700015
dc.identifier.issn0167-4889
2011 Impact Factor: 5.538
2011 SCImago Journal Rankings: 0.707
dc.identifier.issue1-3
dc.identifier.openurl
dc.identifier.pmid12063178
dc.identifier.scopuseid_2-s2.0-0037067180
dc.identifier.spage150
dc.identifier.urihttp://hdl.handle.net/10722/67737
dc.identifier.volume1590
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
dc.publisher.placeNetherlands
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Cell Research
dc.relation.referencesReferences in Scopus
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.
dc.subject.meshAntigens, Polyomavirus Transforming - genetics
dc.subject.meshCell Division
dc.subject.meshCell Line, Transformed
dc.subject.meshChromosomes, Human - genetics
dc.subject.meshEnzyme Activation
dc.subject.meshEpithelial Cells - cytology - drug effects - metabolism
dc.subject.meshGenes, Viral
dc.subject.meshHerpesvirus 4, Human - pathogenicity
dc.subject.meshHumans
dc.subject.meshModels, Biological
dc.subject.meshNasopharyngeal Neoplasms - etiology
dc.subject.meshNasopharynx - cytology - drug effects - metabolism
dc.subject.meshNucleic Acid Hybridization
dc.subject.meshOncogene Proteins, Viral - genetics
dc.subject.meshPapillomavirus E7 Proteins
dc.subject.meshRepressor Proteins
dc.subject.meshSimian virus 40 - genetics
dc.subject.meshTelomerase - metabolism
dc.subject.meshTransforming Growth Factor beta - pharmacology
dc.subject.meshTransforming Growth Factor beta1
dc.subject.meshViral Matrix Proteins - metabolism
dc.titleEstablishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Prince of Wales Hospital Hong Kong
  3. Chinese University of Hong Kong