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Article: Establishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes
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TitleEstablishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes
 
AuthorsTsao, SW1
Wang, X1
Liu, Y1
Cheung, YC1
Feng, H1
Zheng, Z1
Wong, N2
Yuen, PW1
Lo, AKF1
Wong, YC1
Huang, DP3
 
Issue Date2002
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
 
CitationBiochimica Et Biophysica Acta - Molecular Cell Research, 2002, v. 1590 n. 1-3, p. 150-158 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4889(02)00208-2
 
AbstractNasopharyngeal carcinoma (NPC) is a common cancer in Southeast Asia, especially in southern China. One of the most striking features of this disease is its close relationship with Epstein-Barr Virus (EBV). However, to date there is no direct study on the mechanisms involved in the role of EBV in the tumorigenesis of NPC, largely due to lack of an experimental model. Available hypotheses on the association between EBV and NPC are generated from non-nasopharyngeal epithelial cell systems such as human keratinocytes or mouse epithelial cells, which may not truly represent the biological properties of nasopharyngeal epithelial (NP) cells. In this study, we report the establishment of two immortalized NP cell lines, NP69SV40T and NP39E6/E7, using SV40T and HPV16E6/E7 oncogenes. We found that NP60SV40T and NP39E6/E7 cell lines not only maintained many characteristics of normal NP cells (i.e. keratin profile and responsive to TGFβ inhibition) but also highly responsive to one of the EBV encoded genes, LMP1. Comparative genome hybridization (CGH) analysis showed that these two cell lines contained multiple genetic alterations, some of which have been described in NPC. The immortalized NP cell lines are non-tumorigenic and exhibit anchorage-dependent growth. These cell lines may provide a possible cell model system for studying the mechanisms involved in the tumorigenesis of NPC. © 2002 Elsevier Science B.V. All rights reserved.
 
ISSN0167-4889
2013 Impact Factor: 5.297
2013 SCImago Journal Rankings: 2.999
 
DOIhttp://dx.doi.org/10.1016/S0167-4889(02)00208-2
 
ISI Accession Number IDWOS:000176518700015
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTsao, SW
 
dc.contributor.authorWang, X
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorCheung, YC
 
dc.contributor.authorFeng, H
 
dc.contributor.authorZheng, Z
 
dc.contributor.authorWong, N
 
dc.contributor.authorYuen, PW
 
dc.contributor.authorLo, AKF
 
dc.contributor.authorWong, YC
 
dc.contributor.authorHuang, DP
 
dc.date.accessioned2010-09-06T05:57:48Z
 
dc.date.available2010-09-06T05:57:48Z
 
dc.date.issued2002
 
dc.description.abstractNasopharyngeal carcinoma (NPC) is a common cancer in Southeast Asia, especially in southern China. One of the most striking features of this disease is its close relationship with Epstein-Barr Virus (EBV). However, to date there is no direct study on the mechanisms involved in the role of EBV in the tumorigenesis of NPC, largely due to lack of an experimental model. Available hypotheses on the association between EBV and NPC are generated from non-nasopharyngeal epithelial cell systems such as human keratinocytes or mouse epithelial cells, which may not truly represent the biological properties of nasopharyngeal epithelial (NP) cells. In this study, we report the establishment of two immortalized NP cell lines, NP69SV40T and NP39E6/E7, using SV40T and HPV16E6/E7 oncogenes. We found that NP60SV40T and NP39E6/E7 cell lines not only maintained many characteristics of normal NP cells (i.e. keratin profile and responsive to TGFβ inhibition) but also highly responsive to one of the EBV encoded genes, LMP1. Comparative genome hybridization (CGH) analysis showed that these two cell lines contained multiple genetic alterations, some of which have been described in NPC. The immortalized NP cell lines are non-tumorigenic and exhibit anchorage-dependent growth. These cell lines may provide a possible cell model system for studying the mechanisms involved in the tumorigenesis of NPC. © 2002 Elsevier Science B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Cell Research, 2002, v. 1590 n. 1-3, p. 150-158 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4889(02)00208-2
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0167-4889(02)00208-2
 
dc.identifier.epage158
 
dc.identifier.hkuros69736
 
dc.identifier.isiWOS:000176518700015
 
dc.identifier.issn0167-4889
2013 Impact Factor: 5.297
2013 SCImago Journal Rankings: 2.999
 
dc.identifier.issue1-3
 
dc.identifier.openurl
 
dc.identifier.pmid12063178
 
dc.identifier.scopuseid_2-s2.0-0037067180
 
dc.identifier.spage150
 
dc.identifier.urihttp://hdl.handle.net/10722/67737
 
dc.identifier.volume1590
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Cell Research
 
dc.relation.referencesReferences in Scopus
 
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.
 
dc.subject.meshAntigens, Polyomavirus Transforming - genetics
 
dc.subject.meshCell Division
 
dc.subject.meshCell Line, Transformed
 
dc.subject.meshChromosomes, Human - genetics
 
dc.subject.meshEnzyme Activation
 
dc.subject.meshEpithelial Cells - cytology - drug effects - metabolism
 
dc.subject.meshGenes, Viral
 
dc.subject.meshHerpesvirus 4, Human - pathogenicity
 
dc.subject.meshHumans
 
dc.subject.meshModels, Biological
 
dc.subject.meshNasopharyngeal Neoplasms - etiology
 
dc.subject.meshNasopharynx - cytology - drug effects - metabolism
 
dc.subject.meshNucleic Acid Hybridization
 
dc.subject.meshOncogene Proteins, Viral - genetics
 
dc.subject.meshPapillomavirus E7 Proteins
 
dc.subject.meshRepressor Proteins
 
dc.subject.meshSimian virus 40 - genetics
 
dc.subject.meshTelomerase - metabolism
 
dc.subject.meshTransforming Growth Factor beta - pharmacology
 
dc.subject.meshTransforming Growth Factor beta1
 
dc.subject.meshViral Matrix Proteins - metabolism
 
dc.titleEstablishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Prince of Wales Hospital Hong Kong
  3. Chinese University of Hong Kong