File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: c-Jun Expression in Surviving and Regenerating Retinal Ganglion Cells: Effects of Intravitreal Neurotrophic Supply

Titlec-Jun Expression in Surviving and Regenerating Retinal Ganglion Cells: Effects of Intravitreal Neurotrophic Supply
Authors
Issue Date2003
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2003, v. 44 n. 12, p. 5342-5348 How to Cite?
AbstractPURPOSE. To investigate c-jun expression in surviving and axonregenerating retinal ganglion cells (RGCs) and the effect of intravitreal neurotrophic supply on c-jun expression. METHODS. All animals underwent optic nerve transection (ONT) 0.5 mm behind the eyeball. Some animals underwent a replacement of the optic nerve with an autologous sciatic nerve graft (SNG) to allow axonal regrowth. To provide a neurotrophic supply, a peripheral nerve (PN) segment or brain-derived neurotrophic factor (BDNF)/ciliary neurotrophic factor (CNTF) was applied intravitreally. The time course of c-jun expression was first examined in both surviving and regenerating RGCs. Then, c-jun expression was examined in surviving and regenerating RGCs 3 weeks after intravitreal BDNF/CNTF treatment. Animals with vehicle eye injection were used as the control. Fluorescent dye was used for retrograde labeling of surviving (applied behind the eyeball) and regenerating (applied at the distal end of the SNG) RGCs. All retinas were immunohistochemically stained for c-jun. RESULTS. c-Jun was not detected in normal RGCs, but weak expression was seen in surviving RGCs after ON injury. The proportion of c-jun-positive (+) RGCs among surviving cell population was 52.6% to 86.5% 2 to 6 weeks after ONT. Among regenerating RGCs, more than 80% expressed c-jun in all treatment groups, a proportion that was significantly higher after CNTF treatment (90.7%). In addition, c-jun expression was much stronger in intensity and the c-jun + nuclei were much larger in regenerating than in surviving RGCs. CONCLUSIONS. c-Jun expression in RGCs was upregulated after injury. Most regenerating RGCs were c-jun+, and the intensity of c-jun expression was higher in regenerating than in surviving RGCs. CNTF also upregulated c-jun expression in RGCs.
Persistent Identifierhttp://hdl.handle.net/10722/67728
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Qen_HK
dc.contributor.authorCui, Qen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-06T05:57:43Z-
dc.date.available2010-09-06T05:57:43Z-
dc.date.issued2003en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2003, v. 44 n. 12, p. 5342-5348en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67728-
dc.description.abstractPURPOSE. To investigate c-jun expression in surviving and axonregenerating retinal ganglion cells (RGCs) and the effect of intravitreal neurotrophic supply on c-jun expression. METHODS. All animals underwent optic nerve transection (ONT) 0.5 mm behind the eyeball. Some animals underwent a replacement of the optic nerve with an autologous sciatic nerve graft (SNG) to allow axonal regrowth. To provide a neurotrophic supply, a peripheral nerve (PN) segment or brain-derived neurotrophic factor (BDNF)/ciliary neurotrophic factor (CNTF) was applied intravitreally. The time course of c-jun expression was first examined in both surviving and regenerating RGCs. Then, c-jun expression was examined in surviving and regenerating RGCs 3 weeks after intravitreal BDNF/CNTF treatment. Animals with vehicle eye injection were used as the control. Fluorescent dye was used for retrograde labeling of surviving (applied behind the eyeball) and regenerating (applied at the distal end of the SNG) RGCs. All retinas were immunohistochemically stained for c-jun. RESULTS. c-Jun was not detected in normal RGCs, but weak expression was seen in surviving RGCs after ON injury. The proportion of c-jun-positive (+) RGCs among surviving cell population was 52.6% to 86.5% 2 to 6 weeks after ONT. Among regenerating RGCs, more than 80% expressed c-jun in all treatment groups, a proportion that was significantly higher after CNTF treatment (90.7%). In addition, c-jun expression was much stronger in intensity and the c-jun + nuclei were much larger in regenerating than in surviving RGCs. CONCLUSIONS. c-Jun expression in RGCs was upregulated after injury. Most regenerating RGCs were c-jun+, and the intensity of c-jun expression was higher in regenerating than in surviving RGCs. CNTF also upregulated c-jun expression in RGCs.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAxons - physiologyen_HK
dc.subject.meshBrain-Derived Neurotrophic Factor - pharmacologyen_HK
dc.subject.meshCell Survival - physiologyen_HK
dc.subject.meshCiliary Neurotrophic Factor - pharmacologyen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshFluorescent Antibody Technique, Indirecten_HK
dc.subject.meshInjectionsen_HK
dc.subject.meshMesocricetusen_HK
dc.subject.meshOptic Nerve Injuries - metabolismen_HK
dc.subject.meshProto-Oncogene Proteins c-jun - metabolismen_HK
dc.subject.meshRegeneration - physiologyen_HK
dc.subject.meshRetinal Ganglion Cells - drug effects - metabolismen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshUp-Regulationen_HK
dc.subject.meshVitreous Bodyen_HK
dc.titlec-Jun Expression in Surviving and Regenerating Retinal Ganglion Cells: Effects of Intravitreal Neurotrophic Supplyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0146-0404&volume=44 No 12&spage=5342&epage=5348&date=2003&atitle=c-Jun+expression+in+surviving+and+regenerating+retinal+ganglion+cells:+effects+of+intravitreal+neurotrophic+supplyen_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1167/iovs.03-0444en_HK
dc.identifier.pmid14638736-
dc.identifier.scopuseid_2-s2.0-0344851523en_HK
dc.identifier.hkuros85471en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344851523&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume44en_HK
dc.identifier.issue12en_HK
dc.identifier.spage5342en_HK
dc.identifier.epage5348en_HK
dc.identifier.isiWOS:000186801200040-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLu, Q=54902894100en_HK
dc.identifier.scopusauthoridCui, Q=7103080164en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats