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Article: THY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma
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TitleTHY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma
 
AuthorsHong, LL8
Bangarusamy, DK4
Xie, D2 6
Cheung, AKL8
Cheng, Y8 3
Kumaran, MK4 7
Miller, L4
Liu, ETB4
Guan, XY2
Sham, JS2
Fang, Y6
Li, L5
Wang, N5
Protopopov, AI1
Zabarovsky, ER1
Sai, WT2
Stanbridge, EJ9
Lung, ML8
 
KeywordsChromosome 11
Microcell hybrid
Nasopharyngeal carcinoma
Oligo-microarray
THY1
 
Issue Date2005
 
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
 
CitationOncogene, 2005, v. 24 n. 43, p. 6525-6532 [How to Cite?]
DOI: http://dx.doi.org/10.1038/sj.onc.1208812
 
AbstractUsing oligonucleotide microarray analysis, THY1, mapping close to a previously defined 11q22-23 nasopharyngeal carcinoma (NPC) critical region was identified as showing consistent downregulated expression in the tumour segregants, as compared to their parental tumour-suppressing microcell hybrids (MCHs). Gene expression and protein analyses show that THY1 was not expressed in the NPC HONE1 recipient cells, tumour segregants, and other NPC cell lines; THY1 was exclusively expressed in the non-tumourigenic MCHs. The mechanism of THY1 gene inactivation in these cell lines was attributed to hypermethylation. Clinical study showed that in 65% of NPC specimens there was either downregulation or loss of THY1 gene expression. Using a tissue microarray and immunohistochemical staining, 44% of the NPC cases showed downregulated expression of THY1 and 9% lost THY1 expression. The frequency of THY1 downregulated expression in lymph node metastatic NPC was 63%, which was significantly higher than in the primary tumour (33%). After transfection of THY1 gene into HONE1 cells, a dramatic reduction of colony formation ability was observed. These findings suggest that THY1 is a good candidate tumour suppressor gene in NPC, which is significantly associated with lymph node metastases. © 2005 Nature Publishing Group All rights reserved.
 
ISSN0950-9232
2012 Impact Factor: 7.357
2012 SCImago Journal Rankings: 3.558
 
DOIhttp://dx.doi.org/10.1038/sj.onc.1208812
 
ISI Accession Number IDWOS:000232204100006
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHong, LL
 
dc.contributor.authorBangarusamy, DK
 
dc.contributor.authorXie, D
 
dc.contributor.authorCheung, AKL
 
dc.contributor.authorCheng, Y
 
dc.contributor.authorKumaran, MK
 
dc.contributor.authorMiller, L
 
dc.contributor.authorLiu, ETB
 
dc.contributor.authorGuan, XY
 
dc.contributor.authorSham, JS
 
dc.contributor.authorFang, Y
 
dc.contributor.authorLi, L
 
dc.contributor.authorWang, N
 
dc.contributor.authorProtopopov, AI
 
dc.contributor.authorZabarovsky, ER
 
dc.contributor.authorSai, WT
 
dc.contributor.authorStanbridge, EJ
 
dc.contributor.authorLung, ML
 
dc.date.accessioned2010-09-06T05:57:38Z
 
dc.date.available2010-09-06T05:57:38Z
 
dc.date.issued2005
 
dc.description.abstractUsing oligonucleotide microarray analysis, THY1, mapping close to a previously defined 11q22-23 nasopharyngeal carcinoma (NPC) critical region was identified as showing consistent downregulated expression in the tumour segregants, as compared to their parental tumour-suppressing microcell hybrids (MCHs). Gene expression and protein analyses show that THY1 was not expressed in the NPC HONE1 recipient cells, tumour segregants, and other NPC cell lines; THY1 was exclusively expressed in the non-tumourigenic MCHs. The mechanism of THY1 gene inactivation in these cell lines was attributed to hypermethylation. Clinical study showed that in 65% of NPC specimens there was either downregulation or loss of THY1 gene expression. Using a tissue microarray and immunohistochemical staining, 44% of the NPC cases showed downregulated expression of THY1 and 9% lost THY1 expression. The frequency of THY1 downregulated expression in lymph node metastatic NPC was 63%, which was significantly higher than in the primary tumour (33%). After transfection of THY1 gene into HONE1 cells, a dramatic reduction of colony formation ability was observed. These findings suggest that THY1 is a good candidate tumour suppressor gene in NPC, which is significantly associated with lymph node metastases. © 2005 Nature Publishing Group All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationOncogene, 2005, v. 24 n. 43, p. 6525-6532 [How to Cite?]
DOI: http://dx.doi.org/10.1038/sj.onc.1208812
 
dc.identifier.citeulike233458
 
dc.identifier.doihttp://dx.doi.org/10.1038/sj.onc.1208812
 
dc.identifier.epage6532
 
dc.identifier.hkuros173237
 
dc.identifier.isiWOS:000232204100006
 
dc.identifier.issn0950-9232
2012 Impact Factor: 7.357
2012 SCImago Journal Rankings: 3.558
 
dc.identifier.issue43
 
dc.identifier.openurl
 
dc.identifier.pmid16007174
 
dc.identifier.scopuseid_2-s2.0-26944465443
 
dc.identifier.spage6525
 
dc.identifier.urihttp://hdl.handle.net/10722/67717
 
dc.identifier.volume24
 
dc.languageeng
 
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofOncogene
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAntigens, Thy-1 - genetics - metabolism
 
dc.subject.meshCarcinoma - genetics - pathology
 
dc.subject.meshChromosomes, Human, Pair 11
 
dc.subject.meshDNA Methylation
 
dc.subject.meshNasopharyngeal Neoplasms - genetics - pathology
 
dc.subjectChromosome 11
 
dc.subjectMicrocell hybrid
 
dc.subjectNasopharyngeal carcinoma
 
dc.subjectOligo-microarray
 
dc.subjectTHY1
 
dc.titleTHY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma
 
dc.typeArticle
 
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Author Affiliations
  1. Institutionen För Mikrobiologi, Tumör-Och Cellbiologi
  2. The University of Hong Kong
  3. National Naval Medical Center
  4. Genome Institute of Singapore
  5. University of Rochester School of Medicine and Dentistry
  6. Sun Yat-Sen University
  7. Imperial College London
  8. Hong Kong University of Science and Technology
  9. UC Irvine