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Article: THY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma

TitleTHY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma
Authors
KeywordsChromosome 11
Microcell hybrid
Nasopharyngeal carcinoma
Oligo-microarray
THY1
Issue Date2005
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2005, v. 24 n. 43, p. 6525-6532 How to Cite?
Abstract
Using oligonucleotide microarray analysis, THY1, mapping close to a previously defined 11q22-23 nasopharyngeal carcinoma (NPC) critical region was identified as showing consistent downregulated expression in the tumour segregants, as compared to their parental tumour-suppressing microcell hybrids (MCHs). Gene expression and protein analyses show that THY1 was not expressed in the NPC HONE1 recipient cells, tumour segregants, and other NPC cell lines; THY1 was exclusively expressed in the non-tumourigenic MCHs. The mechanism of THY1 gene inactivation in these cell lines was attributed to hypermethylation. Clinical study showed that in 65% of NPC specimens there was either downregulation or loss of THY1 gene expression. Using a tissue microarray and immunohistochemical staining, 44% of the NPC cases showed downregulated expression of THY1 and 9% lost THY1 expression. The frequency of THY1 downregulated expression in lymph node metastatic NPC was 63%, which was significantly higher than in the primary tumour (33%). After transfection of THY1 gene into HONE1 cells, a dramatic reduction of colony formation ability was observed. These findings suggest that THY1 is a good candidate tumour suppressor gene in NPC, which is significantly associated with lymph node metastases. © 2005 Nature Publishing Group All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67717
ISSN
2013 Impact Factor: 8.559
2013 SCImago Journal Rankings: 4.764
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHong, LLen_HK
dc.contributor.authorBangarusamy, DKen_HK
dc.contributor.authorXie, Den_HK
dc.contributor.authorCheung, AKLen_HK
dc.contributor.authorCheng, Yen_HK
dc.contributor.authorKumaran, MKen_HK
dc.contributor.authorMiller, Len_HK
dc.contributor.authorLiu, ETBen_HK
dc.contributor.authorGuan, XYen_HK
dc.contributor.authorSham, JSen_HK
dc.contributor.authorFang, Yen_HK
dc.contributor.authorLi, Len_HK
dc.contributor.authorWang, Nen_HK
dc.contributor.authorProtopopov, AIen_HK
dc.contributor.authorZabarovsky, ERen_HK
dc.contributor.authorSai, WTen_HK
dc.contributor.authorStanbridge, EJen_HK
dc.contributor.authorLung, MLen_HK
dc.date.accessioned2010-09-06T05:57:38Z-
dc.date.available2010-09-06T05:57:38Z-
dc.date.issued2005en_HK
dc.identifier.citationOncogene, 2005, v. 24 n. 43, p. 6525-6532en_HK
dc.identifier.issn0950-9232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67717-
dc.description.abstractUsing oligonucleotide microarray analysis, THY1, mapping close to a previously defined 11q22-23 nasopharyngeal carcinoma (NPC) critical region was identified as showing consistent downregulated expression in the tumour segregants, as compared to their parental tumour-suppressing microcell hybrids (MCHs). Gene expression and protein analyses show that THY1 was not expressed in the NPC HONE1 recipient cells, tumour segregants, and other NPC cell lines; THY1 was exclusively expressed in the non-tumourigenic MCHs. The mechanism of THY1 gene inactivation in these cell lines was attributed to hypermethylation. Clinical study showed that in 65% of NPC specimens there was either downregulation or loss of THY1 gene expression. Using a tissue microarray and immunohistochemical staining, 44% of the NPC cases showed downregulated expression of THY1 and 9% lost THY1 expression. The frequency of THY1 downregulated expression in lymph node metastatic NPC was 63%, which was significantly higher than in the primary tumour (33%). After transfection of THY1 gene into HONE1 cells, a dramatic reduction of colony formation ability was observed. These findings suggest that THY1 is a good candidate tumour suppressor gene in NPC, which is significantly associated with lymph node metastases. © 2005 Nature Publishing Group All rights reserved.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_HK
dc.relation.ispartofOncogeneen_HK
dc.subjectChromosome 11en_HK
dc.subjectMicrocell hybriden_HK
dc.subjectNasopharyngeal carcinomaen_HK
dc.subjectOligo-microarrayen_HK
dc.subjectTHY1en_HK
dc.subject.meshAntigens, Thy-1 - genetics - metabolism-
dc.subject.meshCarcinoma - genetics - pathology-
dc.subject.meshChromosomes, Human, Pair 11-
dc.subject.meshDNA Methylation-
dc.subject.meshNasopharyngeal Neoplasms - genetics - pathology-
dc.titleTHY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-9232&volume=24&issue=43&spage=6525&epage=6532&date=2005&atitle=THY1+is+a+candidate+tumour+suppressor+gene+with+decreased+expression+in+metastatic+nasopharyngeal+carcinomaen_HK
dc.identifier.emailHong, LL: hllung2@hku.hken_HK
dc.identifier.emailCheung, AKL: arthurhk@hku.hken_HK
dc.identifier.emailCheng, Y: yuecheng@hku.hken_HK
dc.identifier.emailGuan, XY: xyguan@hkucc.hku.hken_HK
dc.identifier.emailSai, WT: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailLung, ML: mlilung@hku.hken_HK
dc.identifier.authorityHong, LL=rp00299en_HK
dc.identifier.authorityCheung, AKL=rp01769en_HK
dc.identifier.authorityCheng, Y=rp01320en_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.identifier.authoritySai, WT=rp00399en_HK
dc.identifier.authorityLung, ML=rp00300en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/sj.onc.1208812en_HK
dc.identifier.pmid16007174en_HK
dc.identifier.scopuseid_2-s2.0-26944465443en_HK
dc.identifier.hkuros173237en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-26944465443&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue43en_HK
dc.identifier.spage6525en_HK
dc.identifier.epage6532en_HK
dc.identifier.isiWOS:000232204100006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHong, LL=6603819904en_HK
dc.identifier.scopusauthoridBangarusamy, DK=8948733500en_HK
dc.identifier.scopusauthoridXie, D=35070710200en_HK
dc.identifier.scopusauthoridCheung, AKL=8967932600en_HK
dc.identifier.scopusauthoridCheng, Y=36131038300en_HK
dc.identifier.scopusauthoridKumaran, MK=8056747700en_HK
dc.identifier.scopusauthoridMiller, L=55728695600en_HK
dc.identifier.scopusauthoridLiu, ETB=7202240109en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.scopusauthoridSham, JS=8967933200en_HK
dc.identifier.scopusauthoridFang, Y=7403457405en_HK
dc.identifier.scopusauthoridLi, L=16432864000en_HK
dc.identifier.scopusauthoridWang, N=7404340716en_HK
dc.identifier.scopusauthoridProtopopov, AI=7006756529en_HK
dc.identifier.scopusauthoridZabarovsky, ER=7007009108en_HK
dc.identifier.scopusauthoridSai, WT=7102813116en_HK
dc.identifier.scopusauthoridStanbridge, EJ=7103249410en_HK
dc.identifier.scopusauthoridLung, ML=7006411788en_HK
dc.identifier.citeulike233458-

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