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Article: Oxygen-derived free radicals mediate endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes

TitleOxygen-derived free radicals mediate endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes
Authors
KeywordsEndothelium-dependent contractions
Endothelium-derived contracting factor
Oxidative stress
Oxygen-derived free radicals
Streptozotocin-induced diabetes
Issue Date2007
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 2007, v. 152 n. 7, p. 1033-1041 How to Cite?
AbstractBackground and purpose: The present experiments were designed to study the contribution of oxygen-derived free radicals to endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes. Experimental approach: Rings with and without endothelium were suspended in organ chambers for isometric tension recording. The production of oxygen-derived free radicals in the endothelium was measured with 2′,7′- dichlorodihydrofluorescein diacetate using confocal microscopy. The presence of protein was measured by western blotting. Key results: In the presence of L-NAME, the calcium ionophore A23187 induced larger endothelium-dependent contractions in femoral arteries from diabetic rats. Tiron, catalase, deferoxamine and MnTMPyP, but not superoxide dismutase reduced the response, suggesting that oxygen-derived free radicals are involved in the endothelium-dependent contraction. In the presence of L-NAME, A23187 increased the fluorescence signal in femoral arteries from streptozotocin-treated, but not in those from control rats, confirming that the production of oxygen-derived free radicals contributes to the enhanced endothelium-dependent contractions in diabetes. Exogenous H 2O 2 caused contractions in femoral arterial rings without endothelium which were reduced by deferoxamine, indicating that hydroxyl radicals contract vascular smooth muscle and thus could be an endothelium-derived contracting factor in diabetes. The reduced presence of Mn-SOD and the decreased activity of catalase in femoral arteries from streptozotocin-treated rats demonstrated the presence of a redox abnormality in arteries from rats with diabetes. Conclusions and implications: These findings suggest that the redox abnormality resulting from diabetes increases oxidative stress which facilitates and/or causes endothelium-dependent contractions. © 2007 Nature Publishing Group All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67716
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShi, Yen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-09-06T05:57:37Z-
dc.date.available2010-09-06T05:57:37Z-
dc.date.issued2007en_HK
dc.identifier.citationBritish Journal Of Pharmacology, 2007, v. 152 n. 7, p. 1033-1041en_HK
dc.identifier.issn0007-1188en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67716-
dc.description.abstractBackground and purpose: The present experiments were designed to study the contribution of oxygen-derived free radicals to endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes. Experimental approach: Rings with and without endothelium were suspended in organ chambers for isometric tension recording. The production of oxygen-derived free radicals in the endothelium was measured with 2′,7′- dichlorodihydrofluorescein diacetate using confocal microscopy. The presence of protein was measured by western blotting. Key results: In the presence of L-NAME, the calcium ionophore A23187 induced larger endothelium-dependent contractions in femoral arteries from diabetic rats. Tiron, catalase, deferoxamine and MnTMPyP, but not superoxide dismutase reduced the response, suggesting that oxygen-derived free radicals are involved in the endothelium-dependent contraction. In the presence of L-NAME, A23187 increased the fluorescence signal in femoral arteries from streptozotocin-treated, but not in those from control rats, confirming that the production of oxygen-derived free radicals contributes to the enhanced endothelium-dependent contractions in diabetes. Exogenous H 2O 2 caused contractions in femoral arterial rings without endothelium which were reduced by deferoxamine, indicating that hydroxyl radicals contract vascular smooth muscle and thus could be an endothelium-derived contracting factor in diabetes. The reduced presence of Mn-SOD and the decreased activity of catalase in femoral arteries from streptozotocin-treated rats demonstrated the presence of a redox abnormality in arteries from rats with diabetes. Conclusions and implications: These findings suggest that the redox abnormality resulting from diabetes increases oxidative stress which facilitates and/or causes endothelium-dependent contractions. © 2007 Nature Publishing Group All rights reserved.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_HK
dc.relation.ispartofBritish Journal of Pharmacologyen_HK
dc.subjectEndothelium-dependent contractionsen_HK
dc.subjectEndothelium-derived contracting factoren_HK
dc.subjectOxidative stressen_HK
dc.subjectOxygen-derived free radicalsen_HK
dc.subjectStreptozotocin-induced diabetesen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntioxidants - pharmacologyen_HK
dc.subject.meshCalcimycin - pharmacologyen_HK
dc.subject.meshCatalase - drug effects - metabolismen_HK
dc.subject.meshDiabetes Mellitus, Experimental - chemically induced - metabolism - physiopathologyen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshEndothelium, Vascular - drug effects - metabolismen_HK
dc.subject.meshEnzyme Activation - drug effectsen_HK
dc.subject.meshEnzyme Inhibitors - pharmacologyen_HK
dc.subject.meshFemoral Artery - drug effects - metabolism - physiopathologyen_HK
dc.subject.meshHydrogen Peroxide - antagonists & inhibitors - pharmacologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicroscopy, Confocal - methodsen_HK
dc.subject.meshOrgan Culture Techniquesen_HK
dc.subject.meshOxidants - pharmacologyen_HK
dc.subject.meshOxidation-Reductionen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReactive Oxygen Species - metabolismen_HK
dc.subject.meshSignal Transduction - drug effectsen_HK
dc.subject.meshStreptozocinen_HK
dc.subject.meshSuperoxide Dismutase - drug effects - metabolismen_HK
dc.subject.meshVasoconstriction - drug effectsen_HK
dc.titleOxygen-derived free radicals mediate endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1188&volume=152&spage=1033&epage=1041&date=2007&atitle=Oxygen-derived+free+radicals+mediate+endothelium-dependent+contractions+in+femoral+arteries+of+rats+with+streptozotocin-induced+diabetesen_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1038/sj.bjp.0707439en_HK
dc.identifier.pmid17767168-
dc.identifier.pmcidPMC2095103-
dc.identifier.scopuseid_2-s2.0-36448929845en_HK
dc.identifier.hkuros151173en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36448929845&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume152en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1033en_HK
dc.identifier.epage1041en_HK
dc.identifier.isiWOS:000251139600007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridShi, Y=7404964959en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK

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