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Article: Cytogenetic and molecular genetic characterization of immortalized human ovarian surface epithelial cell lines: Consistent loss of chromosome 13 and amplification of chromosome 20

TitleCytogenetic and molecular genetic characterization of immortalized human ovarian surface epithelial cell lines: Consistent loss of chromosome 13 and amplification of chromosome 20
Authors
KeywordsChromosome aberrations
Hsr
Immortalized ovarian cell lines
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 2004, v. 92 n. 1, p. 183-191 How to Cite?
AbstractObjectives. This study aimed at identifying the genetic events involved in immortalization of ovarian epithelial cells, which might be important steps in ovarian carcinogenesis. Methods. The genetic profiles of five human ovarian surface epithelial (HOSE) cell lines immortalized by retroviral transfection of the human papillomavirus (HPV) E6/E7 genes were thoroughly characterized by chromosome banding and fluorescence in situ hybridization (FISH), at various passages pre- and post-crisis. Results. In pre-crisis, most cells had simple, non-clonal karyotypic changes. Telomere association was the commonest aberration, suggesting that tolermase dysfunction might be an important genetic event leading to cellular crisis. After immortalization post-crisis, however, the karyotypic patterns were non-random. Loss of genetic materials was a characteristic feature. The commonest numerical aberrations were -13, -14, -16, -17, -18, and +5. Among them, loss of chromosome 13 was common change observed in all lines. The only recurrent structural aberration was homogeneously staining regions (hsr) observed in three lines. FISH and combined binary ratio labeling (COBRA)-FISH showed in two cases that the hsrs were derived from chromosome 20. Clonal evolution was observed in four of the lines. In one line, hsr was the only change shared by all subclones, suggesting that it might be a primary event in cell immortalization. Conclusion. The results of the present study suggested that loss of chromosome 13 and the amplification of chromosome 20 might be early genetic events involved in ovarian cell immortalization, and might be useful targets for the study of genomic aberrations in ovarian carcinogenesis. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67701
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Yen_HK
dc.contributor.authorZhang, Hen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorJin, Cen_HK
dc.contributor.authorLv, Men_HK
dc.contributor.authorStrömbeck, Ben_HK
dc.contributor.authorWiegant, Jen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorYuen, PWen_HK
dc.contributor.authorKwong, YLen_HK
dc.date.accessioned2010-09-06T05:57:29Z-
dc.date.available2010-09-06T05:57:29Z-
dc.date.issued2004en_HK
dc.identifier.citationGynecologic Oncology, 2004, v. 92 n. 1, p. 183-191en_HK
dc.identifier.issn0090-8258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67701-
dc.description.abstractObjectives. This study aimed at identifying the genetic events involved in immortalization of ovarian epithelial cells, which might be important steps in ovarian carcinogenesis. Methods. The genetic profiles of five human ovarian surface epithelial (HOSE) cell lines immortalized by retroviral transfection of the human papillomavirus (HPV) E6/E7 genes were thoroughly characterized by chromosome banding and fluorescence in situ hybridization (FISH), at various passages pre- and post-crisis. Results. In pre-crisis, most cells had simple, non-clonal karyotypic changes. Telomere association was the commonest aberration, suggesting that tolermase dysfunction might be an important genetic event leading to cellular crisis. After immortalization post-crisis, however, the karyotypic patterns were non-random. Loss of genetic materials was a characteristic feature. The commonest numerical aberrations were -13, -14, -16, -17, -18, and +5. Among them, loss of chromosome 13 was common change observed in all lines. The only recurrent structural aberration was homogeneously staining regions (hsr) observed in three lines. FISH and combined binary ratio labeling (COBRA)-FISH showed in two cases that the hsrs were derived from chromosome 20. Clonal evolution was observed in four of the lines. In one line, hsr was the only change shared by all subclones, suggesting that it might be a primary event in cell immortalization. Conclusion. The results of the present study suggested that loss of chromosome 13 and the amplification of chromosome 20 might be early genetic events involved in ovarian cell immortalization, and might be useful targets for the study of genomic aberrations in ovarian carcinogenesis. © 2003 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_HK
dc.relation.ispartofGynecologic Oncologyen_HK
dc.subjectChromosome aberrationsen_HK
dc.subjectHsren_HK
dc.subjectImmortalized ovarian cell linesen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCell Transformation, Neoplastic - geneticsen_HK
dc.subject.meshChromosome Aberrationsen_HK
dc.subject.meshChromosomes, Human, Pair 13 - geneticsen_HK
dc.subject.meshChromosomes, Human, Pair 20 - geneticsen_HK
dc.subject.meshEpithelial Cells - cytology - physiologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshOvarian Neoplasms - geneticsen_HK
dc.subject.meshOvary - cytology - physiology - ultrastructureen_HK
dc.titleCytogenetic and molecular genetic characterization of immortalized human ovarian surface epithelial cell lines: Consistent loss of chromosome 13 and amplification of chromosome 20en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0090-8258&volume=92&spage=183&epage=191&date=2004&atitle=Cytogenetic+and+molecular+genetic+characterization+of+immortalized+human+ovarian+surface+epithelial+cell+lines:+consistent+loss+of+chromosome+13+and+amplification+of+chromosome+20en_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ygyno.2003.09.007en_HK
dc.identifier.pmid14751156en_HK
dc.identifier.scopuseid_2-s2.0-9144230586en_HK
dc.identifier.hkuros85906en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-9144230586&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume92en_HK
dc.identifier.issue1en_HK
dc.identifier.spage183en_HK
dc.identifier.epage191en_HK
dc.identifier.isiWOS:000189159600028-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridJin, Y=7404457413en_HK
dc.identifier.scopusauthoridZhang, H=14124271200en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridJin, C=7401659093en_HK
dc.identifier.scopusauthoridLv, M=55222421600en_HK
dc.identifier.scopusauthoridStrömbeck, B=6701670678en_HK
dc.identifier.scopusauthoridWiegant, J=7004362680en_HK
dc.identifier.scopusauthoridWan, TSK=25623981600en_HK
dc.identifier.scopusauthoridYuen, PW=7103124007en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK

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