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Article: Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: effects on steroid secretion in rat ovary

TitleCoexpression of adrenomedullin and its receptors in the reproductive system of the rat: effects on steroid secretion in rat ovary
Authors
KeywordsEstradiol
Follicle-stimulating hormone
Human chorionic gonadotropin
Ovary
Progesterone
Issue Date2008
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology of Reproduction, 2008, v. 79 n. 2, p. 200-208 How to Cite?
AbstractThe present study demonstrates the expression of adrenomedullin (ADM) in the reproductive system of the female rat and its effect on the secretion of estradiol and progesterone. Ovarian ADM and Adm mRNA levels were decreased at estrus, whereas oviductal Adm mRNA levels were low at proestrus. Both tissues were shown to coexpress mRNAs encoding the calcitonin receptor-like receptor and receptor activity-modifying protein 1 (Ramp1), Ramp2, and Ramp3. Gel filtration chromatography of ovarian extracts showed two peaks, with the predominant one eluting at the position of authentic rat ADM (1-50) at estrus and at the position of ADM precursor at diestrus. Positive ADM immunostaining was localized in the granulosa and theca cells of the follicle and corpora lutea of the ovary. Adrenomedullin inhibited FSH-induced estradiol secretion in 2-day-old follicles and also suppressed eCG-stimulated progesterone release in corpora lutea. The inhibitory effect of ADM on the follicles and the corpora lutea was abolished by calcitonin gene-related peptide (8-37) and ADM (22-52), respectively. The presence of ADM and the gene expression of ADM and its receptor components in the female reproductive system suggest a paracrine effect of ADM on ovarian steroidogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/67687
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, YYen_HK
dc.contributor.authorLi, Len_HK
dc.contributor.authorHwang, ISSen_HK
dc.contributor.authorTang, Fen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-09-06T05:57:21Z-
dc.date.available2010-09-06T05:57:21Z-
dc.date.issued2008en_HK
dc.identifier.citationBiology of Reproduction, 2008, v. 79 n. 2, p. 200-208en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67687-
dc.description.abstractThe present study demonstrates the expression of adrenomedullin (ADM) in the reproductive system of the female rat and its effect on the secretion of estradiol and progesterone. Ovarian ADM and Adm mRNA levels were decreased at estrus, whereas oviductal Adm mRNA levels were low at proestrus. Both tissues were shown to coexpress mRNAs encoding the calcitonin receptor-like receptor and receptor activity-modifying protein 1 (Ramp1), Ramp2, and Ramp3. Gel filtration chromatography of ovarian extracts showed two peaks, with the predominant one eluting at the position of authentic rat ADM (1-50) at estrus and at the position of ADM precursor at diestrus. Positive ADM immunostaining was localized in the granulosa and theca cells of the follicle and corpora lutea of the ovary. Adrenomedullin inhibited FSH-induced estradiol secretion in 2-day-old follicles and also suppressed eCG-stimulated progesterone release in corpora lutea. The inhibitory effect of ADM on the follicles and the corpora lutea was abolished by calcitonin gene-related peptide (8-37) and ADM (22-52), respectively. The presence of ADM and the gene expression of ADM and its receptor components in the female reproductive system suggest a paracrine effect of ADM on ovarian steroidogenesis.-
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectEstradiol-
dc.subjectFollicle-stimulating hormone-
dc.subjectHuman chorionic gonadotropin-
dc.subjectOvary-
dc.subjectProgesterone-
dc.subject.meshAdrenomedullin - blood - genetics - metabolism-
dc.subject.meshGenitalia, Female - metabolism-
dc.subject.meshGonadal Steroid Hormones - metabolism-
dc.subject.meshOvary - secretion-
dc.subject.meshReceptors, G-Protein-Coupled - genetics - metabolism-
dc.titleCoexpression of adrenomedullin and its receptors in the reproductive system of the rat: effects on steroid secretion in rat ovaryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3363&volume=79&issue=2&spage=200&epage=208&date=2008&atitle=Coexpression+of+adrenomedullin+and+its+receptors+in+the+reproductive+system+of+the+rat:+effects+on+steroid+secretion+in+rat+ovaryen_HK
dc.identifier.emailLi, YY: cyyli@graduate.hku.hken_HK
dc.identifier.emailLi, L: sarabirdlee@gmail.comen_HK
dc.identifier.emailHwang, ISS: isabelbelbel@hotmail.comen_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1095/biolreprod.107.064022-
dc.identifier.pmid18401014-
dc.identifier.scopuseid_2-s2.0-48949100652-
dc.identifier.hkuros148491en_HK
dc.identifier.hkuros151724-
dc.identifier.volume79-
dc.identifier.issue2-
dc.identifier.spage200-
dc.identifier.epage208-
dc.identifier.isiWOS:000257837400003-

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