Article: Differential gene expression in nasopharyngeal carcinoma cells
| Title | Differential gene expression in nasopharyngeal carcinoma cells |
|---|---|
| Authors | Fung, LF1 Lo, AKF1 Yuen, PW1 Liu, Y1 Wang, XH1 Tsao, SW1 |
| Issue Date | 2000 |
| Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie |
| Citation | Life Sciences, 2000, v. 67 n. 8, p. 923-936 [How to Cite?] DOI: http://dx.doi.org/10.1016/S0024-3205(00)00684-6 |
| Abstract | Nasopharyngeal carcinoma (NPC) is a common cancer among southern Chinese. The profile of gene expression in NPC cells is largely unknown. In this study, we have examined differential gene expression in non-malignant and malignant nasopharyngeal epithelial (NPE) cells using a cDNA array hybridization method. A total of 42 genes were identified to be expressed in either non-malignant and malignant NPE cells or both. Thirteen of these genes were overexpressed in malignant NPE cells. These includes nuclear factor (NF90), FOS-related antigen 1 (FRA-1), cytoplasmic dynein light chain (HDLC1), replication factor C (RFC1), nucleoside diphosphate kinase B, UV excision repair protein (RAD23A), insulin-like growth factor receptor II, transcription initiation factor TFIID subunit (TAFII31), growth factor receptor-bound protein 2 (GRB2), UV excision repair protein (RAD23B), glutathione peroxidase. Y box binding protein 1 and heat shock protein 86. In contrast, expression of nine genes was suppressed in malignant NPE cells. These includes calgranulin A, calgranulin B, neutrophil activating protein (ENA-78), heat shock protein 27, integrin beta-1, integrin beta-4, cyclin- dependent kinase inhibitor 1A (p21), interleukin-8 and tyrosine protein kinase receptor (RET). Differential expression of calgranulin A, calgranulin B, ENA-78, FRA-1 and NF90 in non-malignant and malignant nasopharyngeal epithelial cells was confirmed by RT-PCR analysis. (C) 2000 Elsevier Science Inc. |
| ISSN | 0024-3205 2011 Impact Factor: 2.527 2011 SCImago Journal Rankings: 0.189 |
| DOI | http://dx.doi.org/10.1016/S0024-3205(00)00684-6 |
| ISI Accession Number ID | WOS:000088400500008 |
| References | References in Scopus |
| dc.contributor.author | Fung, LF |
|---|---|
| dc.contributor.author | Lo, AKF |
| dc.contributor.author | Yuen, PW |
| dc.contributor.author | Liu, Y |
| dc.contributor.author | Wang, XH |
| dc.contributor.author | Tsao, SW |
| dc.date.accessioned | 2010-09-06T05:57:20Z |
| dc.date.available | 2010-09-06T05:57:20Z |
| dc.date.issued | 2000 |
| dc.description.abstract | Nasopharyngeal carcinoma (NPC) is a common cancer among southern Chinese. The profile of gene expression in NPC cells is largely unknown. In this study, we have examined differential gene expression in non-malignant and malignant nasopharyngeal epithelial (NPE) cells using a cDNA array hybridization method. A total of 42 genes were identified to be expressed in either non-malignant and malignant NPE cells or both. Thirteen of these genes were overexpressed in malignant NPE cells. These includes nuclear factor (NF90), FOS-related antigen 1 (FRA-1), cytoplasmic dynein light chain (HDLC1), replication factor C (RFC1), nucleoside diphosphate kinase B, UV excision repair protein (RAD23A), insulin-like growth factor receptor II, transcription initiation factor TFIID subunit (TAFII31), growth factor receptor-bound protein 2 (GRB2), UV excision repair protein (RAD23B), glutathione peroxidase. Y box binding protein 1 and heat shock protein 86. In contrast, expression of nine genes was suppressed in malignant NPE cells. These includes calgranulin A, calgranulin B, neutrophil activating protein (ENA-78), heat shock protein 27, integrin beta-1, integrin beta-4, cyclin- dependent kinase inhibitor 1A (p21), interleukin-8 and tyrosine protein kinase receptor (RET). Differential expression of calgranulin A, calgranulin B, ENA-78, FRA-1 and NF90 in non-malignant and malignant nasopharyngeal epithelial cells was confirmed by RT-PCR analysis. (C) 2000 Elsevier Science Inc. |
| dc.description.nature | Link_to_subscribed_fulltext |
| dc.identifier.citation | Life Sciences, 2000, v. 67 n. 8, p. 923-936 [How to Cite?] DOI: http://dx.doi.org/10.1016/S0024-3205(00)00684-6 |
| dc.identifier.doi | http://dx.doi.org/10.1016/S0024-3205(00)00684-6 |
| dc.identifier.epage | 936 |
| dc.identifier.hkuros | 52803 |
| dc.identifier.isi | WOS:000088400500008 |
| dc.identifier.issn | 0024-3205 2011 Impact Factor: 2.527 2011 SCImago Journal Rankings: 0.189 |
| dc.identifier.issue | 8 |
| dc.identifier.openurl | ![]() |
| dc.identifier.pmid | 10946852 |
| dc.identifier.scopus | eid_2-s2.0-0034647587 |
| dc.identifier.spage | 923 |
| dc.identifier.uri | http://hdl.handle.net/10722/67686 |
| dc.identifier.volume | 67 |
| dc.language | eng |
| dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie |
| dc.publisher.place | United States |
| dc.relation.ispartof | Life Sciences |
| dc.relation.references | References in Scopus |
| dc.rights | Life Sciences. Copyright © Elsevier Inc. |
| dc.subject.mesh | Calcium-Binding Proteins - genetics |
| dc.subject.mesh | Calgranulin A |
| dc.subject.mesh | Calgranulin B |
| dc.subject.mesh | Chemokine CXCL5 |
| dc.subject.mesh | Chemokines, CXC |
| dc.subject.mesh | DNA, Complementary - analysis |
| dc.subject.mesh | DNA-Binding Proteins - genetics |
| dc.subject.mesh | Gene Expression Regulation, Neoplastic |
| dc.subject.mesh | Humans |
| dc.subject.mesh | Interleukin-8 - analogs & derivatives - genetics |
| dc.subject.mesh | NFATC Transcription Factors |
| dc.subject.mesh | Nasopharyngeal Neoplasms - genetics |
| dc.subject.mesh | Nasopharynx - metabolism |
| dc.subject.mesh | Nuclear Factor 90 Proteins |
| dc.subject.mesh | Nuclear Proteins |
| dc.subject.mesh | Proto-Oncogene Proteins c-fos - genetics |
| dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction |
| dc.subject.mesh | Transcription Factors - genetics |
| dc.subject.mesh | Tumor Cells, Cultured |
| dc.title | Differential gene expression in nasopharyngeal carcinoma cells |
| dc.type | Article |
Author Affiliations
- The University of Hong Kong


