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Article: Intravitreous injection for establishing ocular diseases model

TitleIntravitreous injection for establishing ocular diseases model
Authors
KeywordsEye
Injection
Neuroscience
Rat
Issue Date2007
PublisherJournal of Visualized Experiments. The Journal's web site is located at http://wwwjovecom
Citation
Journal Of Visualized Experiments, 2007 n. 8, article no. e313 How to Cite?
AbstractIntravitreous injection is a widely used technique in visual sciences research. It can be used to establish animal models with ocular diseases or as direct application of local treatment. This video introduces how to use simple and inexpensive tools to finish the intravitreous injection procedure. Use of a 1 ml syringe, instead of a hemilton syringe, is used. Practical tips for how to make appropriate injection needles using glass pipettes with perfect tips, and how to easily connect the syringe needle with the glass pipette tightly together, are given. To conduct a good intravitreous injection, there are three aspects to be observed: 1) injection site should not disrupt retina structure; 2) bleeding should be avoided to reduce the risk of infection; 3) lens should be untouched to avoid traumatic cataract. In brief, the most important point is to reduce the interruption of normal ocular structure. To avoid interruption of retina, the superior nasal region of rat eye was chosen. Also, the puncture point of the needle was at the par planar, which was about 1.5 mm from the limbal region of the rat eye. A small amount of vitreous is gently pushed out through the puncture hole to reduce the intraocular pressure before injection. With the 45° injection angle, it is less likely to cause traumatic cataract in the rat eye, thus avoiding related complications and influence from lenticular factors. In this operation, there was no cutting of the conjunctiva and ocular muscle, no bleeding. With quick and minor injury, a successful intravitreous injection can be done in minutes. The injection set outlined in this particular protocol is specific for intravitreous injection. However, the methods and materials presented here can also be used for other injection procedures in drug delivery to the brain, spinal cord or other organs in small mammals. © JoVE 2006-2011 All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67669
ISSN
2015 Impact Factor: 1.113
2015 SCImago Journal Rankings: 0.451
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorChiu, Ken_HK
dc.contributor.authorChang, RCCen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-06T05:57:11Z-
dc.date.available2010-09-06T05:57:11Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Visualized Experiments, 2007 n. 8, article no. e313en_HK
dc.identifier.issn1940-087Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67669-
dc.description.abstractIntravitreous injection is a widely used technique in visual sciences research. It can be used to establish animal models with ocular diseases or as direct application of local treatment. This video introduces how to use simple and inexpensive tools to finish the intravitreous injection procedure. Use of a 1 ml syringe, instead of a hemilton syringe, is used. Practical tips for how to make appropriate injection needles using glass pipettes with perfect tips, and how to easily connect the syringe needle with the glass pipette tightly together, are given. To conduct a good intravitreous injection, there are three aspects to be observed: 1) injection site should not disrupt retina structure; 2) bleeding should be avoided to reduce the risk of infection; 3) lens should be untouched to avoid traumatic cataract. In brief, the most important point is to reduce the interruption of normal ocular structure. To avoid interruption of retina, the superior nasal region of rat eye was chosen. Also, the puncture point of the needle was at the par planar, which was about 1.5 mm from the limbal region of the rat eye. A small amount of vitreous is gently pushed out through the puncture hole to reduce the intraocular pressure before injection. With the 45° injection angle, it is less likely to cause traumatic cataract in the rat eye, thus avoiding related complications and influence from lenticular factors. In this operation, there was no cutting of the conjunctiva and ocular muscle, no bleeding. With quick and minor injury, a successful intravitreous injection can be done in minutes. The injection set outlined in this particular protocol is specific for intravitreous injection. However, the methods and materials presented here can also be used for other injection procedures in drug delivery to the brain, spinal cord or other organs in small mammals. © JoVE 2006-2011 All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherJournal of Visualized Experiments. The Journal's web site is located at http://wwwjovecomen_HK
dc.relation.ispartofJournal of Visualized Experimentsen_HK
dc.subjectEyeen_HK
dc.subjectInjectionen_HK
dc.subjectNeuroscienceen_HK
dc.subjectRaten_HK
dc.titleIntravitreous injection for establishing ocular diseases modelen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1940-087X&volume=8&spage=313&epage=&date=2008&atitle=Intravitreous+injection+for+establishing+ocular+diseases+model.en_HK
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3791/313en_HK
dc.identifier.pmid18989417-
dc.identifier.pmcidPMC2562493-
dc.identifier.scopuseid_2-s2.0-80055108151en_HK
dc.identifier.hkuros160620en_HK
dc.identifier.hkuros139423-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80055108151&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.issue8en_HK
dc.identifier.spagearticle no. e313-
dc.identifier.epagearticle no. e313-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChiu, K=15076970500en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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