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Article: Embryos sired by males without accessory sex glands induce failure of uterine support: A study of VEGF, MMP and TGF expression in the golden hamster

TitleEmbryos sired by males without accessory sex glands induce failure of uterine support: A study of VEGF, MMP and TGF expression in the golden hamster
Authors
Issue Date2003
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00429/index.htm
Citation
Anatomy And Embryology, 2003, v. 206 n. 3, p. 203-213 How to Cite?
AbstractTo account for reproductive failure induced by surgical deletion of paternal accessory sex glands in the golden hamster in vivo, we studied expression of vegf, FLT-1 (VEGF-R1), FLK-1 (VEGF-R2), MMP and TGF-β in endometrium of the dam and sired embryos during 5-7 days post coitum by immunohistochemistry, in situ hybridisation, semiquantitative RT-PCR and enzyme-linked immunosorbent assay. Spatiotemporal pattern of vegf expression in the control animals was similar to that reported for intact animals by our group. Removal of paternal ampullary glands did not disturb the normal expression pattern. Removal of ventral prostate glands alone or all accessory sex glands was associated with reduction of vegf transcripts and protein levels in both the embryo and endometrium. FLT-1, FLK-1 and MMP-2 were also reduced. MMP-1 was not changed whereas TGF-β1 expression was enhanced. There was no expression in endometrium in between implantation sites. Thus the implanted embryos had a trophic effect on growth factor production by the endometrium, and the levels of expression were determined by viability and structural integrity of the conceptus. Based on these findings we concluded that incompetent embryos sired by males without the ventral prostate gland or all accessory sex glands reduced the potential of the uterus to support pregnancy. A negative cycle of events was thus set up and eventually led to premature termination of pregnancies.
Persistent Identifierhttp://hdl.handle.net/10722/67654
ISSN
2008 Impact Factor: 1.39
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChow, PHen_HK
dc.contributor.authorJiang, HYen_HK
dc.contributor.authorPoon, HKen_HK
dc.contributor.authorLee, KHen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-09-06T05:57:04Z-
dc.date.available2010-09-06T05:57:04Z-
dc.date.issued2003en_HK
dc.identifier.citationAnatomy And Embryology, 2003, v. 206 n. 3, p. 203-213en_HK
dc.identifier.issn0340-2061en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67654-
dc.description.abstractTo account for reproductive failure induced by surgical deletion of paternal accessory sex glands in the golden hamster in vivo, we studied expression of vegf, FLT-1 (VEGF-R1), FLK-1 (VEGF-R2), MMP and TGF-β in endometrium of the dam and sired embryos during 5-7 days post coitum by immunohistochemistry, in situ hybridisation, semiquantitative RT-PCR and enzyme-linked immunosorbent assay. Spatiotemporal pattern of vegf expression in the control animals was similar to that reported for intact animals by our group. Removal of paternal ampullary glands did not disturb the normal expression pattern. Removal of ventral prostate glands alone or all accessory sex glands was associated with reduction of vegf transcripts and protein levels in both the embryo and endometrium. FLT-1, FLK-1 and MMP-2 were also reduced. MMP-1 was not changed whereas TGF-β1 expression was enhanced. There was no expression in endometrium in between implantation sites. Thus the implanted embryos had a trophic effect on growth factor production by the endometrium, and the levels of expression were determined by viability and structural integrity of the conceptus. Based on these findings we concluded that incompetent embryos sired by males without the ventral prostate gland or all accessory sex glands reduced the potential of the uterus to support pregnancy. A negative cycle of events was thus set up and eventually led to premature termination of pregnancies.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00429/index.htmen_HK
dc.relation.ispartofAnatomy and Embryologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshEmbryo Implantation - physiologyen_HK
dc.subject.meshEmbryo, Mammalian - metabolism - physiopathology - secretionen_HK
dc.subject.meshEndometrium - cytology - growth & development - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGenitalia, Male - injuries - metabolism - secretionen_HK
dc.subject.meshGrowth Substances - metabolism - secretionen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLectins - diagnostic useen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMaternal-Fetal Exchange - physiologyen_HK
dc.subject.meshMatrix Metalloproteinases - metabolismen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshProstate - metabolism - secretionen_HK
dc.subject.meshSeminal Vesicles - metabolism - secretionen_HK
dc.subject.meshTransforming Growth Factor beta - metabolismen_HK
dc.subject.meshVas Deferens - metabolism - secretionen_HK
dc.subject.meshVascular Endothelial Growth Factor Receptor-1 - metabolismen_HK
dc.subject.meshVascular Endothelial Growth Factor Receptor-2 - metabolismen_HK
dc.titleEmbryos sired by males without accessory sex glands induce failure of uterine support: A study of VEGF, MMP and TGF expression in the golden hamsteren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0340-2061&volume=206&spage=203&epage=213&date=2003&atitle=Embryos+sired+by+males+without+accessory+sex+glands+induce+failure+of+uterine+support:+a+study+of+VEGF,+MMP+and+TGF+expression+in+the+golden+hamsteren_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00429-002-0290-5-
dc.identifier.pmid12592572-
dc.identifier.scopuseid_2-s2.0-0037293906en_HK
dc.identifier.hkuros75826en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037293906&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume206en_HK
dc.identifier.issue3en_HK
dc.identifier.spage203en_HK
dc.identifier.epage213en_HK
dc.identifier.isiWOS:000181659800007-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK
dc.identifier.scopusauthoridJiang, HY=34769714800en_HK
dc.identifier.scopusauthoridPoon, HK=7007103075en_HK
dc.identifier.scopusauthoridLee, KH=7501505976en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK

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