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Article: Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons

TitleTransgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons
Authors
Issue Date2005
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2005, v. 46 n. 10, p. 3515-3520 How to Cite?
AbstractPURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation. Copyright © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/67627
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, XMen_HK
dc.contributor.authorChen, BYen_HK
dc.contributor.authorNg, AHLen_HK
dc.contributor.authorTanner, JAen_HK
dc.contributor.authorTay, Den_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorRachel, RAen_HK
dc.contributor.authorCopeland, NGen_HK
dc.contributor.authorJenkins, NAen_HK
dc.contributor.authorHuang, JDen_HK
dc.date.accessioned2010-09-06T05:56:49Z-
dc.date.available2010-09-06T05:56:49Z-
dc.date.issued2005en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2005, v. 46 n. 10, p. 3515-3520en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67627-
dc.description.abstractPURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation. Copyright © Association for Research in Vision and Ophthalmology.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshChromosomes, Artificial, Bacterialen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFluorescent Antibody Technique, Indirecten_HK
dc.subject.meshGalactosides - metabolismen_HK
dc.subject.meshGreen Fluorescent Proteins - genetics - metabolismen_HK
dc.subject.meshGuanine Nucleotide Exchange Factorsen_HK
dc.subject.meshIndoles - metabolismen_HK
dc.subject.meshIntegrases - genetics - metabolismen_HK
dc.subject.meshInterneurons - enzymologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C3Hen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshMice, Knockouten_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshNeuropeptides - geneticsen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshPurkinje Cells - enzymologyen_HK
dc.subject.meshRetinal Rod Photoreceptor Cells - cytology - embryologyen_HK
dc.subject.meshbeta-Galactosidase - metabolismen_HK
dc.titleTransgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neuronsen_HK
dc.typeArticleen_HK
dc.identifier.emailTanner, JA:jatanner@hku.hken_HK
dc.identifier.emailTay, D:dkctay@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.authorityTanner, JA=rp00495en_HK
dc.identifier.authorityTay, D=rp00336en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1167/iovs.04-1201en_HK
dc.identifier.pmid16186328en_HK
dc.identifier.scopuseid_2-s2.0-32944457757en_HK
dc.identifier.hkuros107078en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-32944457757&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue10en_HK
dc.identifier.spage3515en_HK
dc.identifier.epage3520en_HK
dc.identifier.isiWOS:000232112900009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, XM=7410273116en_HK
dc.identifier.scopusauthoridChen, BY=14051424300en_HK
dc.identifier.scopusauthoridNg, AHL=12445077000en_HK
dc.identifier.scopusauthoridTanner, JA=35513993000en_HK
dc.identifier.scopusauthoridTay, D=7006796825en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridRachel, RA=7003812116en_HK
dc.identifier.scopusauthoridCopeland, NG=35374759300en_HK
dc.identifier.scopusauthoridJenkins, NA=35379887700en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK

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