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Article: Clinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma

TitleClinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma
Authors
Issue Date2000
Citation
Applied Immunohistochemistry And Molecular Morphology, 2000, v. 8 n. 4, p. 300-309 How to Cite?
AbstractThree different in situ hybridization (ISH) methods were compared for their clinical relevance and suitability in detecting human papillomavirus (HPV) 16/18 in 55 cases of squamous cell carcinoma (SCC) of the uterine cervix. After the initial biopsy, surgery, and/or radiation therapy, patients were followed for 5 to 8 years. A biotinylated cDNA probe for HPV 16/18 was applied to serial sections in combination with conventional streptavidin-biotin-peroxidase ISH (a widely applied routine procedure), streptavidin-Nanogold-silver ISH, and tyramide-signal amplified (TSA) streptavidin-Nanogold-gold ISH. The TSA principle is also known as catalyzed reporter deposition and is, apart from in situ PCR, probably today's most sensitive technique for detecting papillomavirus infection by microscopic means. Nearly 65.5% of the cases showed specific HPV 16/18 detection with TSA ISH, whereas 43.6% were positive with streptavidin-Nanogold-silver-ISH, and only 40.0% with peroxidase-based ISH. Statistical analyses comparing early and advanced stages in both HPV-positive and -negative groups revealed a significantly better outcome for early disease patients; statistical significance was most pronounced with TSA ISH. In a subgroup of patients who had received radiation therapy without prior surgery (n = 35), those with advanced disease were significantly less likely to have HPV 16/18 infection than those with early disease. A significantly better overall survival was observed in those women with HPV 16/18-positive carcinomas who had undergone surgery before radiation therapy (seen with all three methods). We conclude that TSA, in addition to being the most sensitive HPV in situ method applied in this study, gave the most significant and clinically relevant statistical results.
Persistent Identifierhttp://hdl.handle.net/10722/67615
ISSN
2012 Impact Factor: 1.828
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGraf, AHen_HK
dc.contributor.authorCheung, ALMen_HK
dc.contributor.authorHauserKronberger, Cen_HK
dc.contributor.authorDandachi, Nen_HK
dc.contributor.authorTubbs, RRen_HK
dc.contributor.authorDietze, Oen_HK
dc.contributor.authorHacker, GWen_HK
dc.date.accessioned2010-09-06T05:56:42Z-
dc.date.available2010-09-06T05:56:42Z-
dc.date.issued2000en_HK
dc.identifier.citationApplied Immunohistochemistry And Molecular Morphology, 2000, v. 8 n. 4, p. 300-309en_HK
dc.identifier.issn1062-3345en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67615-
dc.description.abstractThree different in situ hybridization (ISH) methods were compared for their clinical relevance and suitability in detecting human papillomavirus (HPV) 16/18 in 55 cases of squamous cell carcinoma (SCC) of the uterine cervix. After the initial biopsy, surgery, and/or radiation therapy, patients were followed for 5 to 8 years. A biotinylated cDNA probe for HPV 16/18 was applied to serial sections in combination with conventional streptavidin-biotin-peroxidase ISH (a widely applied routine procedure), streptavidin-Nanogold-silver ISH, and tyramide-signal amplified (TSA) streptavidin-Nanogold-gold ISH. The TSA principle is also known as catalyzed reporter deposition and is, apart from in situ PCR, probably today's most sensitive technique for detecting papillomavirus infection by microscopic means. Nearly 65.5% of the cases showed specific HPV 16/18 detection with TSA ISH, whereas 43.6% were positive with streptavidin-Nanogold-silver-ISH, and only 40.0% with peroxidase-based ISH. Statistical analyses comparing early and advanced stages in both HPV-positive and -negative groups revealed a significantly better outcome for early disease patients; statistical significance was most pronounced with TSA ISH. In a subgroup of patients who had received radiation therapy without prior surgery (n = 35), those with advanced disease were significantly less likely to have HPV 16/18 infection than those with early disease. A significantly better overall survival was observed in those women with HPV 16/18-positive carcinomas who had undergone surgery before radiation therapy (seen with all three methods). We conclude that TSA, in addition to being the most sensitive HPV in situ method applied in this study, gave the most significant and clinically relevant statistical results.en_HK
dc.languageengen_HK
dc.relation.ispartofApplied Immunohistochemistry and Molecular Morphologyen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAged, 80 and overen_HK
dc.subject.meshBiotin - analogs & derivatives - metabolismen_HK
dc.subject.meshCarcinoma, Squamous Cell - metabolism - mortality - therapy - virologyen_HK
dc.subject.meshDNA, Complementary - metabolismen_HK
dc.subject.meshDisease-Free Survivalen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFollow-Up Studiesen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshIndicators and Reagents - metabolismen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPapillomaviridae - metabolismen_HK
dc.subject.meshPeroxidase - metabolismen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSilver - metabolismen_HK
dc.subject.meshStreptavidin - metabolismen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshTyramine - analogs & derivatives - metabolismen_HK
dc.subject.meshUterine Cervical Neoplasms - metabolism - mortality - therapy - virologyen_HK
dc.titleClinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailCheung, ALM:lmcheung@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ALM=rp00332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/00022744-200012000-00007en_HK
dc.identifier.pmid11127922-
dc.identifier.scopuseid_2-s2.0-0033723802en_HK
dc.identifier.hkuros56145en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033723802&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.issue4en_HK
dc.identifier.spage300en_HK
dc.identifier.epage309en_HK
dc.identifier.isiWOS:000165547800007-
dc.identifier.scopusauthoridGraf, AH=7006625267en_HK
dc.identifier.scopusauthoridCheung, ALM=7401806497en_HK
dc.identifier.scopusauthoridHauserKronberger, C=7003360268en_HK
dc.identifier.scopusauthoridDandachi, N=9238457200en_HK
dc.identifier.scopusauthoridTubbs, RR=35400790200en_HK
dc.identifier.scopusauthoridDietze, O=24798646300en_HK
dc.identifier.scopusauthoridHacker, GW=7006705677en_HK

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