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Article: Chondroitinase ABC promotes axonal regeneration of Clarke's neurons after spinal cord injury

TitleChondroitinase ABC promotes axonal regeneration of Clarke's neurons after spinal cord injury
Authors
KeywordsAxonal Regeneration
Clarke's nucleus
Proteoglycans
Scar
Spinal cord
Issue Date2000
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.neuroreport.com
Citation
Neuroreport, 2000, v. 11 n. 5, p. 1063-1067 How to Cite?
AbstractWe examined whether enzymatic digestion of chondroitin sulfate (CS) promoted the axonal regeneration of neurons in Clarke's nucleus (CN) into a peripheral nerve (PN) graft following injury of the spinal cord. After hemisection at TII, a segment of PN graft was implanted at the lesion site. Either vehicle, brain-derived neurotrophic factor (BDNF) or chondroitinase ABC was applied at the implantation site. The postoperative survival period was 4 weeks. Treatment with vehicle or BDNF did not promote the axonal regeneration of CN neurons into the PN graft. Application of 2.5 unit/ml chondroitinase ABC resulted in a significant increase (12.8%) in the number of regenerated CN neurons. Double labeling with Fluoro-Gold and NADPH- diaphorase histochemistry showed that the regenerated CN neurons did not express nitric oxide synthase (NOS). Our results suggest that CS is inhibitory to the regeneration of CN neurons following injury of the spinal cord. (C) 2000 Lippincott Williams and Wilkins.
Persistent Identifierhttp://hdl.handle.net/10722/67607
ISSN
2015 Impact Factor: 1.343
2015 SCImago Journal Rankings: 0.783
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYick, LWen_HK
dc.contributor.authorWu, Wen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-06T05:56:38Z-
dc.date.available2010-09-06T05:56:38Z-
dc.date.issued2000en_HK
dc.identifier.citationNeuroreport, 2000, v. 11 n. 5, p. 1063-1067en_HK
dc.identifier.issn0959-4965en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67607-
dc.description.abstractWe examined whether enzymatic digestion of chondroitin sulfate (CS) promoted the axonal regeneration of neurons in Clarke's nucleus (CN) into a peripheral nerve (PN) graft following injury of the spinal cord. After hemisection at TII, a segment of PN graft was implanted at the lesion site. Either vehicle, brain-derived neurotrophic factor (BDNF) or chondroitinase ABC was applied at the implantation site. The postoperative survival period was 4 weeks. Treatment with vehicle or BDNF did not promote the axonal regeneration of CN neurons into the PN graft. Application of 2.5 unit/ml chondroitinase ABC resulted in a significant increase (12.8%) in the number of regenerated CN neurons. Double labeling with Fluoro-Gold and NADPH- diaphorase histochemistry showed that the regenerated CN neurons did not express nitric oxide synthase (NOS). Our results suggest that CS is inhibitory to the regeneration of CN neurons following injury of the spinal cord. (C) 2000 Lippincott Williams and Wilkins.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.neuroreport.comen_HK
dc.relation.ispartofNeuroReporten_HK
dc.rightsNeuroReport. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subjectAxonal Regenerationen_HK
dc.subjectClarke's nucleusen_HK
dc.subjectProteoglycansen_HK
dc.subjectScaren_HK
dc.subjectSpinal corden_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAxons - drug effects - metabolism - ultrastructureen_HK
dc.subject.meshBrain-Derived Neurotrophic Factor - pharmacologyen_HK
dc.subject.meshChondroitin ABC Lyase - pharmacologyen_HK
dc.subject.meshChondroitin Sulfates - metabolismen_HK
dc.subject.meshDenervation - adverse effectsen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFluorescent Dyes - diagnostic useen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshNADPH Dehydrogenase - analysisen_HK
dc.subject.meshNerve Regeneration - drug effects - physiologyen_HK
dc.subject.meshNitric Oxide Synthase - metabolismen_HK
dc.subject.meshPeripheral Nerves - drug effects - metabolism - transplantationen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshSpinal Cord - drug effects - pathology - physiopathologyen_HK
dc.subject.meshSpinal Cord Injuries - drug therapy - pathology - physiopathologyen_HK
dc.subject.meshSpinocerebellar Tracts - drug effects - injuries - pathologyen_HK
dc.subject.meshStilbamidinesen_HK
dc.titleChondroitinase ABC promotes axonal regeneration of Clarke's neurons after spinal cord injuryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0959-4965&volume=11 No5&spage=1063&epage=1067&date=2000&atitle=Chondroitinase+ABC+promotes+axonal+regeneration+of+Clarke%27s+neurons+after+spinal+cord+injuryen_HK
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityWu, W=rp00419en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10790883-
dc.identifier.scopuseid_2-s2.0-0000137795en_HK
dc.identifier.hkuros51525en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0000137795&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1063en_HK
dc.identifier.epage1067en_HK
dc.identifier.isiWOS:000086385300036-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYick, LW=6603414804en_HK
dc.identifier.scopusauthoridWu, W=7407081122en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK

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