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Article: Intravitreal transplants of Schwann cells and fibroblasts promote the survival of axotomized retinal ganglion cells in rats

TitleIntravitreal transplants of Schwann cells and fibroblasts promote the survival of axotomized retinal ganglion cells in rats
Authors
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2004, v. 1029 n. 1, p. 56-64 How to Cite?
AbstractSchwann cells (SCs) are considered one of the major cellular components to maintain the integrity of the peripheral nervous system (PNS) neurons after injury. Intravitreal transplant of peripheral nerves or Schwann cells has been shown to enhance the regenerative ability of retinal ganglion cells (RGCs). In the present study, we compared the effects of intravitreal transplants of Schwann cells and fibroblasts, two major components of peripheral nerves, on the survival of retinal ganglion cells in adult rats after optic nerve (ON) transection. Purified Schwann cells and fibroblasts from neonatal sciatic nerves were injected into the vitreous body of adult rats. Three days after the injection, the optic nerves were transected intraorbitally. After 1 week or 1 month, surviving retinal ganglion cells were retrogradely labelled with Fluoro-Gold (FG) and the number of surviving retinal ganglion cells was counted. The retinas were further processed for 200-kDa neurofilament RT-97 immunohistochemistry. It was found that intravitreally injected- Schwann cells and -fibroblasts delayed the death of axotomized retinal ganglion cells for 1 week. In addition, in the animal group with 1 month survival time after optic nerve transection, those received a larger number of Schwann cells had more surviving retinal ganglion cells and more profusely ramified axonal processes near the optic disc. These findings reveal that both Schwann cells and fibroblasts isolated from the peripheral nerve can promote retinal ganglion cell survival after optic nerve transection, presumably by secreting neurotrophic factors. In addition, the data also demonstrate that Schwann cells could promote intraretinal axonal sprouting. Our findings demonstrate a remarkable glial source of neurotrophic factors with potential clinical applications, as autologous Schwann cells and fibroblasts can be feasibly obtained from peripheral nerves. © 2004 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67598
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Sen_HK
dc.contributor.authorHu, Ben_HK
dc.contributor.authorTay, Den_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYip, HKFen_HK
dc.date.accessioned2010-09-06T05:56:33Z-
dc.date.available2010-09-06T05:56:33Z-
dc.date.issued2004en_HK
dc.identifier.citationBrain Research, 2004, v. 1029 n. 1, p. 56-64en_HK
dc.identifier.issn0006-8993en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67598-
dc.description.abstractSchwann cells (SCs) are considered one of the major cellular components to maintain the integrity of the peripheral nervous system (PNS) neurons after injury. Intravitreal transplant of peripheral nerves or Schwann cells has been shown to enhance the regenerative ability of retinal ganglion cells (RGCs). In the present study, we compared the effects of intravitreal transplants of Schwann cells and fibroblasts, two major components of peripheral nerves, on the survival of retinal ganglion cells in adult rats after optic nerve (ON) transection. Purified Schwann cells and fibroblasts from neonatal sciatic nerves were injected into the vitreous body of adult rats. Three days after the injection, the optic nerves were transected intraorbitally. After 1 week or 1 month, surviving retinal ganglion cells were retrogradely labelled with Fluoro-Gold (FG) and the number of surviving retinal ganglion cells was counted. The retinas were further processed for 200-kDa neurofilament RT-97 immunohistochemistry. It was found that intravitreally injected- Schwann cells and -fibroblasts delayed the death of axotomized retinal ganglion cells for 1 week. In addition, in the animal group with 1 month survival time after optic nerve transection, those received a larger number of Schwann cells had more surviving retinal ganglion cells and more profusely ramified axonal processes near the optic disc. These findings reveal that both Schwann cells and fibroblasts isolated from the peripheral nerve can promote retinal ganglion cell survival after optic nerve transection, presumably by secreting neurotrophic factors. In addition, the data also demonstrate that Schwann cells could promote intraretinal axonal sprouting. Our findings demonstrate a remarkable glial source of neurotrophic factors with potential clinical applications, as autologous Schwann cells and fibroblasts can be feasibly obtained from peripheral nerves. © 2004 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_HK
dc.relation.ispartofBrain Researchen_HK
dc.rightsBrain Research. Copyright © Elsevier BV.en_HK
dc.subject.meshAnalysis of Varianceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAxotomyen_HK
dc.subject.meshCell Survivalen_HK
dc.subject.meshFibroblasts - transplantationen_HK
dc.subject.meshNerve Regeneration - physiologyen_HK
dc.subject.meshOptic Nerveen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshRetinal Ganglion Cells - cytology - physiologyen_HK
dc.subject.meshSchwann Cells - transplantationen_HK
dc.subject.meshSciatic Nerve - cytology - transplantationen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshVitreous Body - cytology - surgeryen_HK
dc.titleIntravitreal transplants of Schwann cells and fibroblasts promote the survival of axotomized retinal ganglion cells in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=1029&spage=56&epage=64&date=2004&atitle=Intravitreal+transplants+of+Schwann+cells+and+fibroblasts+promote+the+survival+of+axotomized+retinal+ganglion+cells+in+ratsen_HK
dc.identifier.emailTay, D:dkctay@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailYip, HKF:hkfyip@hku.hken_HK
dc.identifier.authorityTay, D=rp00336en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityYip, HKF=rp00285en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.brainres.2004.09.038en_HK
dc.identifier.pmid15533316-
dc.identifier.scopuseid_2-s2.0-7744228098en_HK
dc.identifier.hkuros96365en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-7744228098&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1029en_HK
dc.identifier.issue1en_HK
dc.identifier.spage56en_HK
dc.identifier.epage64en_HK
dc.identifier.isiWOS:000225481700007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, S=23497101600en_HK
dc.identifier.scopusauthoridHu, B=35733928400en_HK
dc.identifier.scopusauthoridTay, D=7006796825en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridYip, HKF=7101980864en_HK

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