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Article: Over expression of ID-1 in prostate cancer

TitleOver expression of ID-1 in prostate cancer
Authors
Issue Date2002
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.elsevier.com/locate/juro
Citation
Journal Of Urology, 2002, v. 167 n. 6, p. 2598-2602 How to Cite?
AbstractPurpose: The helix-loop-helix protein Id-1 serves to prevent basic helix-loop-helix transcription factors from binding to DNA, thus, inhibiting the transcription of differentiation associated genes. Over expression of Id-1 has been reported in certain tumors, such as breast, esophageal, pancreatic and medullary thyroid cancers. In Noble rats we have previously demonstrated that up-regulation of Id-1 is closely associated with the development of sex hormone induced prostate cancers. Therefore, we hypothesized that over expression of Id-1 would also occur in human prostate cancer and Id-1 protein may serve as a potential marker for prostate carcinogenesis. To test this hypothesis we analyzed Id-1 messenger RNA and protein expression by in situ hybridization and immunohistochemical study in human normal prostate, benign prostatic hyperplasia (BPH) and prostate cancer tissues. Materials and Methods: Pathological specimens were obtained from 19 patients with BPH and 47 with prostate carcinoma, representing a complete range of Gleason grades. A total of 12 normal prostate tissue specimens were used for comparison. Immunohistochemical study was performed using the polyclonal antibody against human Id-1 protein and an RNA probe was generated from Id-1 complementary DNA for in situ hybridization. Results: Negative to weak expression of Id-1 in normal prostate or BPH tissue was observed on immunohistochemical study and in situ hybridization. In contrast, all prostate cancer biopsies showed significant positive Id-1 expression in tumor cells at the messenger RNA and protein levels. Furthermore, Id-1 expression was stronger in poorly differentiated than in well differentiated carcinomas, suggesting that the level of Id-1 expression may be associated with tumor malignancy. Conclusions: Our results suggest that over expression of Id-1 may have important roles in the development of prostate cancer. The potential use of Id-1 protein as a marker for prostate cancer should be further explored.
Persistent Identifierhttp://hdl.handle.net/10722/67596
ISSN
2015 Impact Factor: 4.7
2015 SCImago Journal Rankings: 2.259
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorOuyang, XSen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorLee, DTWen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorWong, YCen_HK
dc.date.accessioned2010-09-06T05:56:32Z-
dc.date.available2010-09-06T05:56:32Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Urology, 2002, v. 167 n. 6, p. 2598-2602en_HK
dc.identifier.issn0022-5347en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67596-
dc.description.abstractPurpose: The helix-loop-helix protein Id-1 serves to prevent basic helix-loop-helix transcription factors from binding to DNA, thus, inhibiting the transcription of differentiation associated genes. Over expression of Id-1 has been reported in certain tumors, such as breast, esophageal, pancreatic and medullary thyroid cancers. In Noble rats we have previously demonstrated that up-regulation of Id-1 is closely associated with the development of sex hormone induced prostate cancers. Therefore, we hypothesized that over expression of Id-1 would also occur in human prostate cancer and Id-1 protein may serve as a potential marker for prostate carcinogenesis. To test this hypothesis we analyzed Id-1 messenger RNA and protein expression by in situ hybridization and immunohistochemical study in human normal prostate, benign prostatic hyperplasia (BPH) and prostate cancer tissues. Materials and Methods: Pathological specimens were obtained from 19 patients with BPH and 47 with prostate carcinoma, representing a complete range of Gleason grades. A total of 12 normal prostate tissue specimens were used for comparison. Immunohistochemical study was performed using the polyclonal antibody against human Id-1 protein and an RNA probe was generated from Id-1 complementary DNA for in situ hybridization. Results: Negative to weak expression of Id-1 in normal prostate or BPH tissue was observed on immunohistochemical study and in situ hybridization. In contrast, all prostate cancer biopsies showed significant positive Id-1 expression in tumor cells at the messenger RNA and protein levels. Furthermore, Id-1 expression was stronger in poorly differentiated than in well differentiated carcinomas, suggesting that the level of Id-1 expression may be associated with tumor malignancy. Conclusions: Our results suggest that over expression of Id-1 may have important roles in the development of prostate cancer. The potential use of Id-1 protein as a marker for prostate cancer should be further explored.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.elsevier.com/locate/juroen_HK
dc.relation.ispartofJournal of Urologyen_HK
dc.rightsThe Journal of Urology. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshDNA-Binding Proteins - analysis - geneticsen_HK
dc.subject.meshHelix-Loop-Helix Motifsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshInhibitor of Differentiation Protein 1en_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshProstate - metabolismen_HK
dc.subject.meshProstatic Hyperplasia - metabolismen_HK
dc.subject.meshProstatic Neoplasms - metabolismen_HK
dc.subject.meshRNA, Messenger - analysisen_HK
dc.subject.meshRepressor Proteinsen_HK
dc.subject.meshTranscription Factors - analysis - geneticsen_HK
dc.subject.meshTumor Markers, Biological - analysisen_HK
dc.titleOver expression of ID-1 in prostate canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-5347&volume=167&spage=2598&epage=2602&date=2002&atitle=Over+expression+of+ID-1+in+prostate+canceren_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11992094en_HK
dc.identifier.scopuseid_2-s2.0-0036093103en_HK
dc.identifier.hkuros66209en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036093103&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume167en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2598en_HK
dc.identifier.epage2602en_HK
dc.identifier.isiWOS:000175602500069-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridOuyang, XS=8711278300en_HK
dc.identifier.scopusauthoridWang, X=7501854829en_HK
dc.identifier.scopusauthoridLee, DTW=7406666118en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK

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