File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Age-related changes in adrenomedullin expression and hypoxia-inducible factor-1 activity in the rat lung and their responses to hypoxia

TitleAge-related changes in adrenomedullin expression and hypoxia-inducible factor-1 activity in the rat lung and their responses to hypoxia
Authors
Issue Date2007
PublisherOxford University Press. The Journal's web site is located at http://biomed.gerontologyjournals.org/
Citation
4th Symposium of Adrenomedullin and Proadenomedullin N-20 Peptide, Zurich, Switzerland, 2004. In Journals Of Gerontology - Series A Biological Sciences And Medical Sciences, 2007, v. 62 n. 1, p. 41-49 How to Cite?
AbstractMale rats aged 3 months, 12 months and 20 months were subjected to breathing 8% oxygen for 6 hours. Lung preproadrenomedullin (AM) messenger RNA (mRNA) levels were measured by solution hybridization-RNase protection assay while AM was measured by radioimmunoassay. The binding of hypoxia-inducible factor-1α (HIF-1α) to DNA was determined by electrophoretic mobility shift. There was an age-related increase in basal levels of preproAM mRNA and AM and of the binding of hypoxia-inducible factor (HIF) to DNA. Upon hypoxic stimulation, HIF binding to DNA increased in the young and middle-aged rats, but not in the old rats. AM gene expression increased in response to hypoxia in rats of all ages, but the increase was much less in the old rats. AM peptide levels in the lung decreased with age in hypoxia. In a separate experiment, male rats aged 3 months and 20 months were subjected to hypoxia as described above. PreproAM, calcitonin receptor-like receptor (CRLR), receptor activity modifying protein (RAMP) mRNA, HIF-1 and peptidyl-glycine-amidating monooxygenase (PAM) mRNA levels were measured by reverse transcription-polymerase chain reaction. All except PAM showed a decrease in basal levels and a diminished response to hypoxia in the old rats. Polysome profiling demonstrated decreases in the percentages of translatable preproAM mRNA in response to hypoxia, with a greater decrease in the old than the young rats. It is concluded that an age-dependent decrease in the hypoxic response of the AM system in the lung was associated with high basal levels of HIF activity and AM expression in the old rats, and a lower proportion of translatable preproAM mRNA in the old rats in response to hypoxia. Thus, the HIF-AM pathway may be impaired in the aged lung, and other mechanisms may be present to maintain an AM response to hypoxia. Copyright 2007 by The Gerontological Society of America.
Persistent Identifierhttp://hdl.handle.net/10722/67594
ISSN
2015 Impact Factor: 5.476
2015 SCImago Journal Rankings: 2.675
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHwang, ISSen_HK
dc.contributor.authorFung, MLen_HK
dc.contributor.authorLiong, ECen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorTang, Fen_HK
dc.date.accessioned2010-09-06T05:56:31Z-
dc.date.available2010-09-06T05:56:31Z-
dc.date.issued2007en_HK
dc.identifier.citation4th Symposium of Adrenomedullin and Proadenomedullin N-20 Peptide, Zurich, Switzerland, 2004. In Journals Of Gerontology - Series A Biological Sciences And Medical Sciences, 2007, v. 62 n. 1, p. 41-49en_HK
dc.identifier.issn1079-5006en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67594-
dc.description.abstractMale rats aged 3 months, 12 months and 20 months were subjected to breathing 8% oxygen for 6 hours. Lung preproadrenomedullin (AM) messenger RNA (mRNA) levels were measured by solution hybridization-RNase protection assay while AM was measured by radioimmunoassay. The binding of hypoxia-inducible factor-1α (HIF-1α) to DNA was determined by electrophoretic mobility shift. There was an age-related increase in basal levels of preproAM mRNA and AM and of the binding of hypoxia-inducible factor (HIF) to DNA. Upon hypoxic stimulation, HIF binding to DNA increased in the young and middle-aged rats, but not in the old rats. AM gene expression increased in response to hypoxia in rats of all ages, but the increase was much less in the old rats. AM peptide levels in the lung decreased with age in hypoxia. In a separate experiment, male rats aged 3 months and 20 months were subjected to hypoxia as described above. PreproAM, calcitonin receptor-like receptor (CRLR), receptor activity modifying protein (RAMP) mRNA, HIF-1 and peptidyl-glycine-amidating monooxygenase (PAM) mRNA levels were measured by reverse transcription-polymerase chain reaction. All except PAM showed a decrease in basal levels and a diminished response to hypoxia in the old rats. Polysome profiling demonstrated decreases in the percentages of translatable preproAM mRNA in response to hypoxia, with a greater decrease in the old than the young rats. It is concluded that an age-dependent decrease in the hypoxic response of the AM system in the lung was associated with high basal levels of HIF activity and AM expression in the old rats, and a lower proportion of translatable preproAM mRNA in the old rats in response to hypoxia. Thus, the HIF-AM pathway may be impaired in the aged lung, and other mechanisms may be present to maintain an AM response to hypoxia. Copyright 2007 by The Gerontological Society of America.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://biomed.gerontologyjournals.org/en_HK
dc.relation.ispartofJournals of Gerontology - Series A Biological Sciences and Medical Sciencesen_HK
dc.subject.meshAdrenomedullin - genetics - metabolismen_HK
dc.subject.meshAging - genetics - metabolismen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnoxia - genetics - metabolism - pathologyen_HK
dc.subject.meshCalcitonin Receptor-Like Proteinen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshElectrophoresisen_HK
dc.subject.meshGene Expression Regulation, Developmentalen_HK
dc.subject.meshHypoxia-Inducible Factor 1 - biosynthesis - geneticsen_HK
dc.subject.meshLung - metabolism - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMixed Function Oxygenases - geneticsen_HK
dc.subject.meshMultienzyme Complexes - geneticsen_HK
dc.subject.meshPolyribosomes - metabolismen_HK
dc.subject.meshProtein Precursors - geneticsen_HK
dc.subject.meshRNA, Messenger - geneticsen_HK
dc.subject.meshRadioimmunoassayen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReceptors, Calcitonin - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshXenopus Proteinsen_HK
dc.titleAge-related changes in adrenomedullin expression and hypoxia-inducible factor-1 activity in the rat lung and their responses to hypoxiaen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0163-4372&volume=62A&issue=1&spage=41&epage=49&date=2007&atitle=Age-related+changes+in+adrenomedullin+expression+and+hypoxia-inducible+factor-1+activity+in+the+rat+lung+and+their+responses+to+hypoxia.en_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid17301036-
dc.identifier.scopuseid_2-s2.0-34047182083en_HK
dc.identifier.hkuros128585en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34047182083&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume62en_HK
dc.identifier.issue1en_HK
dc.identifier.spage41en_HK
dc.identifier.epage49en_HK
dc.identifier.isiWOS:000247220200006-
dc.publisher.placeUnited Statesen_HK
dc.description.other4th Symposium of Adrenomedullin and Proadenomedullin N-20 Peptide, Zurich, Switzerland, 2004. In Journals Of Gerontology - Series A Biological Sciences And Medical Sciences, 2007, v. 62 n. 1, p. 41-49-
dc.identifier.scopusauthoridHwang, ISS=7201615103en_HK
dc.identifier.scopusauthoridFung, ML=7101955092en_HK
dc.identifier.scopusauthoridLiong, EC=6602732210en_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats