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Article: Amplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells
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TitleAmplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells
 
AuthorsChung, CM2
Man, C2 1
Jin, Y1
Jin, C1
Guan, XY2
Wang, Q2
Wan, TSK2
Cheung, ALM2
Tsao, SW2
 
Keywords20q amplification
Aurora kinase A
HOSE
Immortalization
Ovarian
 
Issue Date2005
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/
 
CitationMolecular Carcinogenesis, 2005, v. 43 n. 3, p. 165-174 [How to Cite?]
DOI: http://dx.doi.org/10.1002/mc.20098
 
AbstractImmortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non-random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including TGIF2 (20q11.2-q12), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and AURKA (20q13.2-q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre-crisis (preimmortalized) and post-crisis (immortalized) HOSE cells. Overexpression of AURKA (Aurora kinase A), also known as BTAK and STK15, by both real time-quantitative polymerase chain reaction (RT-QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of AIB1 and ZNF217 was observed in two of four immortalized HOSE cells examined. Overexpression of TGIF2 and PTPN1 was not significant in our immortalized HOSE cell systems. The degree of overexpression of AURKA was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled P1 artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2-13.3) where AURKA resides. DNA amplification of AURKA was also confirmed using semi-quantitative PCR. Our study showed that amplification and overexpression of AURKA is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis. © 2005 Wiley-Liss, Inc.
 
ISSN0899-1987
2013 Impact Factor: 4.770
 
DOIhttp://dx.doi.org/10.1002/mc.20098
 
ISI Accession Number IDWOS:000230269600005
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChung, CM
 
dc.contributor.authorMan, C
 
dc.contributor.authorJin, Y
 
dc.contributor.authorJin, C
 
dc.contributor.authorGuan, XY
 
dc.contributor.authorWang, Q
 
dc.contributor.authorWan, TSK
 
dc.contributor.authorCheung, ALM
 
dc.contributor.authorTsao, SW
 
dc.date.accessioned2010-09-06T05:56:30Z
 
dc.date.available2010-09-06T05:56:30Z
 
dc.date.issued2005
 
dc.description.abstractImmortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non-random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including TGIF2 (20q11.2-q12), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and AURKA (20q13.2-q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre-crisis (preimmortalized) and post-crisis (immortalized) HOSE cells. Overexpression of AURKA (Aurora kinase A), also known as BTAK and STK15, by both real time-quantitative polymerase chain reaction (RT-QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of AIB1 and ZNF217 was observed in two of four immortalized HOSE cells examined. Overexpression of TGIF2 and PTPN1 was not significant in our immortalized HOSE cell systems. The degree of overexpression of AURKA was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled P1 artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2-13.3) where AURKA resides. DNA amplification of AURKA was also confirmed using semi-quantitative PCR. Our study showed that amplification and overexpression of AURKA is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis. © 2005 Wiley-Liss, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationMolecular Carcinogenesis, 2005, v. 43 n. 3, p. 165-174 [How to Cite?]
DOI: http://dx.doi.org/10.1002/mc.20098
 
dc.identifier.doihttp://dx.doi.org/10.1002/mc.20098
 
dc.identifier.epage174
 
dc.identifier.hkuros150521
 
dc.identifier.hkuros108889
 
dc.identifier.isiWOS:000230269600005
 
dc.identifier.issn0899-1987
2013 Impact Factor: 4.770
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid15880741
 
dc.identifier.scopuseid_2-s2.0-21744461116
 
dc.identifier.spage165
 
dc.identifier.urihttp://hdl.handle.net/10722/67591
 
dc.identifier.volume43
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofMolecular Carcinogenesis
 
dc.relation.referencesReferences in Scopus
 
dc.rightsMolecular Carcinogenesis. Copyright © John Wiley & Sons, Inc.
 
dc.subject.meshBase Sequence
 
dc.subject.meshCell Cycle Proteins - genetics
 
dc.subject.meshCells, Cultured
 
dc.subject.meshChromosome Mapping
 
dc.subject.meshChromosomes, Human, Pair 20 - genetics
 
dc.subject.meshDNA Primers
 
dc.subject.meshEpithelial Cells - enzymology
 
dc.subject.meshFemale
 
dc.subject.meshGene Amplification
 
dc.subject.meshGene Expression Regulation, Enzymologic
 
dc.subject.meshHumans
 
dc.subject.meshIn Situ Hybridization, Fluorescence
 
dc.subject.meshOvary - cytology - enzymology
 
dc.subject.meshPolymerase Chain Reaction
 
dc.subject.meshProtein Kinases - genetics
 
dc.subject.meshProtein-Serine-Threonine Kinases
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshXenopus Proteins - genetics
 
dc.subject20q amplification
 
dc.subjectAurora kinase A
 
dc.subjectHOSE
 
dc.subjectImmortalization
 
dc.subjectOvarian
 
dc.titleAmplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells
 
dc.typeArticle
 
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<contributor.author>Guan, XY</contributor.author>
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<description.abstract>Immortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non-random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including TGIF2 (20q11.2-q12), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and AURKA (20q13.2-q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre-crisis (preimmortalized) and post-crisis (immortalized) HOSE cells. Overexpression of AURKA (Aurora kinase A), also known as BTAK and STK15, by both real time-quantitative polymerase chain reaction (RT-QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of AIB1 and ZNF217 was observed in two of four immortalized HOSE cells examined. Overexpression of TGIF2 and PTPN1 was not significant in our immortalized HOSE cell systems. The degree of overexpression of AURKA was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled P1 artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2-13.3) where AURKA resides. DNA amplification of AURKA was also confirmed using semi-quantitative PCR. Our study showed that amplification and overexpression of AURKA is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis. &#169; 2005 Wiley-Liss, Inc.</description.abstract>
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<subject>20q amplification</subject>
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<subject>Immortalization</subject>
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Author Affiliations
  1. Universitssjukhuset i Lund
  2. The University of Hong Kong