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Article: Amplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells

TitleAmplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells
Authors
Keywords20q amplification
Aurora kinase A
HOSE
Immortalization
Ovarian
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/
Citation
Molecular Carcinogenesis, 2005, v. 43 n. 3, p. 165-174 How to Cite?
AbstractImmortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non-random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including TGIF2 (20q11.2-q12), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and AURKA (20q13.2-q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre-crisis (preimmortalized) and post-crisis (immortalized) HOSE cells. Overexpression of AURKA (Aurora kinase A), also known as BTAK and STK15, by both real time-quantitative polymerase chain reaction (RT-QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of AIB1 and ZNF217 was observed in two of four immortalized HOSE cells examined. Overexpression of TGIF2 and PTPN1 was not significant in our immortalized HOSE cell systems. The degree of overexpression of AURKA was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled P1 artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2-13.3) where AURKA resides. DNA amplification of AURKA was also confirmed using semi-quantitative PCR. Our study showed that amplification and overexpression of AURKA is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis. © 2005 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/67591
ISSN
2014 Impact Factor: 4.808
2014 SCImago Journal Rankings: 1.397
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChung, CMen_HK
dc.contributor.authorMan, Cen_HK
dc.contributor.authorJin, Yen_HK
dc.contributor.authorJin, Cen_HK
dc.contributor.authorGuan, XYen_HK
dc.contributor.authorWang, Qen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorCheung, ALMen_HK
dc.contributor.authorTsao, SWen_HK
dc.date.accessioned2010-09-06T05:56:30Z-
dc.date.available2010-09-06T05:56:30Z-
dc.date.issued2005en_HK
dc.identifier.citationMolecular Carcinogenesis, 2005, v. 43 n. 3, p. 165-174en_HK
dc.identifier.issn0899-1987en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67591-
dc.description.abstractImmortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non-random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including TGIF2 (20q11.2-q12), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and AURKA (20q13.2-q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre-crisis (preimmortalized) and post-crisis (immortalized) HOSE cells. Overexpression of AURKA (Aurora kinase A), also known as BTAK and STK15, by both real time-quantitative polymerase chain reaction (RT-QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of AIB1 and ZNF217 was observed in two of four immortalized HOSE cells examined. Overexpression of TGIF2 and PTPN1 was not significant in our immortalized HOSE cell systems. The degree of overexpression of AURKA was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled P1 artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2-13.3) where AURKA resides. DNA amplification of AURKA was also confirmed using semi-quantitative PCR. Our study showed that amplification and overexpression of AURKA is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis. © 2005 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/en_HK
dc.relation.ispartofMolecular Carcinogenesisen_HK
dc.rightsMolecular Carcinogenesis. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject20q amplificationen_HK
dc.subjectAurora kinase Aen_HK
dc.subjectHOSEen_HK
dc.subjectImmortalizationen_HK
dc.subjectOvarianen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCell Cycle Proteins - geneticsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshChromosome Mappingen_HK
dc.subject.meshChromosomes, Human, Pair 20 - geneticsen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshEpithelial Cells - enzymologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Amplificationen_HK
dc.subject.meshGene Expression Regulation, Enzymologicen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshOvary - cytology - enzymologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshProtein Kinases - geneticsen_HK
dc.subject.meshProtein-Serine-Threonine Kinasesen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshXenopus Proteins - geneticsen_HK
dc.titleAmplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0899-1987&volume=43&issue=3&spage=165&epage=174&date=2005&atitle=Amplification+And+Overexpression+Of+Aurora+Kinase+A+(aurka)+In+Immortalized+Human+Ovarian+Epithelial+(hose)+Cells.+en_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ALM:lmcheung@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.identifier.authorityCheung, ALM=rp00332en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mc.20098en_HK
dc.identifier.pmid15880741en_HK
dc.identifier.scopuseid_2-s2.0-21744461116en_HK
dc.identifier.hkuros150521en_HK
dc.identifier.hkuros108889-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-21744461116&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue3en_HK
dc.identifier.spage165en_HK
dc.identifier.epage174en_HK
dc.identifier.isiWOS:000230269600005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChung, CM=8630943000en_HK
dc.identifier.scopusauthoridMan, C=7005722377en_HK
dc.identifier.scopusauthoridJin, Y=7404457413en_HK
dc.identifier.scopusauthoridJin, C=7401659093en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.scopusauthoridWang, Q=7406910452en_HK
dc.identifier.scopusauthoridWan, TSK=25623981600en_HK
dc.identifier.scopusauthoridCheung, ALM=7401806497en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK

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