Article: Amplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells

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TitleAmplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells
AuthorsChung, CM2
Man, C1 2
Jin, Y1
Jin, C1
Guan, XY2
Wang, Q2
Wan, TSK2
Cheung, ALM2
Tsao, SW2
Keywords20q amplification
Aurora kinase A
HOSE
Immortalization
Ovarian
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/
CitationMolecular Carcinogenesis, 2005, v. 43 n. 3, p. 165-174 [How to Cite?]
DOI: http://dx.doi.org/10.1002/mc.20098
AbstractImmortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non-random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including TGIF2 (20q11.2-q12), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and AURKA (20q13.2-q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre-crisis (preimmortalized) and post-crisis (immortalized) HOSE cells. Overexpression of AURKA (Aurora kinase A), also known as BTAK and STK15, by both real time-quantitative polymerase chain reaction (RT-QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of AIB1 and ZNF217 was observed in two of four immortalized HOSE cells examined. Overexpression of TGIF2 and PTPN1 was not significant in our immortalized HOSE cell systems. The degree of overexpression of AURKA was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled P1 artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2-13.3) where AURKA resides. DNA amplification of AURKA was also confirmed using semi-quantitative PCR. Our study showed that amplification and overexpression of AURKA is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis. © 2005 Wiley-Liss, Inc.
ISSN0899-1987
2011 Impact Factor: 3.164
2011 SCImago Journal Rankings: 0.347
DOIhttp://dx.doi.org/10.1002/mc.20098
ISI Accession Number IDWOS:000230269600005
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChung, CM
dc.contributor.authorMan, C
dc.contributor.authorJin, Y
dc.contributor.authorJin, C
dc.contributor.authorGuan, XY
dc.contributor.authorWang, Q
dc.contributor.authorWan, TSK
dc.contributor.authorCheung, ALM
dc.contributor.authorTsao, SW
dc.date.accessioned2010-09-06T05:56:30Z
dc.date.available2010-09-06T05:56:30Z
dc.date.issued2005
dc.description.abstractImmortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non-random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including TGIF2 (20q11.2-q12), AIB1 (20q12), PTPN1 (20q13.1), ZNF217 (20q13.2), and AURKA (20q13.2-q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre-crisis (preimmortalized) and post-crisis (immortalized) HOSE cells. Overexpression of AURKA (Aurora kinase A), also known as BTAK and STK15, by both real time-quantitative polymerase chain reaction (RT-QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of AIB1 and ZNF217 was observed in two of four immortalized HOSE cells examined. Overexpression of TGIF2 and PTPN1 was not significant in our immortalized HOSE cell systems. The degree of overexpression of AURKA was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled P1 artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2-13.3) where AURKA resides. DNA amplification of AURKA was also confirmed using semi-quantitative PCR. Our study showed that amplification and overexpression of AURKA is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis. © 2005 Wiley-Liss, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationMolecular Carcinogenesis, 2005, v. 43 n. 3, p. 165-174 [How to Cite?]
DOI: http://dx.doi.org/10.1002/mc.20098
dc.identifier.doihttp://dx.doi.org/10.1002/mc.20098
dc.identifier.epage174
dc.identifier.hkuros150521
dc.identifier.hkuros108889
dc.identifier.isiWOS:000230269600005
dc.identifier.issn0899-1987
2011 Impact Factor: 3.164
2011 SCImago Journal Rankings: 0.347
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmid15880741
dc.identifier.scopuseid_2-s2.0-21744461116
dc.identifier.spage165
dc.identifier.urihttp://hdl.handle.net/10722/67591
dc.identifier.volume43
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/
dc.publisher.placeUnited States
dc.relation.ispartofMolecular Carcinogenesis
dc.relation.referencesReferences in Scopus
dc.rightsMolecular Carcinogenesis. Copyright © John Wiley & Sons, Inc.
dc.subject.meshBase Sequence
dc.subject.meshCell Cycle Proteins - genetics
dc.subject.meshCells, Cultured
dc.subject.meshChromosome Mapping
dc.subject.meshChromosomes, Human, Pair 20 - genetics
dc.subject.meshDNA Primers
dc.subject.meshEpithelial Cells - enzymology
dc.subject.meshFemale
dc.subject.meshGene Amplification
dc.subject.meshGene Expression Regulation, Enzymologic
dc.subject.meshHumans
dc.subject.meshIn Situ Hybridization, Fluorescence
dc.subject.meshOvary - cytology - enzymology
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshProtein Kinases - genetics
dc.subject.meshProtein-Serine-Threonine Kinases
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshXenopus Proteins - genetics
dc.subject20q amplification
dc.subjectAurora kinase A
dc.subjectHOSE
dc.subjectImmortalization
dc.subjectOvarian
dc.titleAmplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells
dc.typeArticle
Author Affiliations
  1. Universitssjukhuset i Lund
  2. The University of Hong Kong