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Article: A subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats
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TitleA subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats
 
AuthorsYuan, Q1
Scott, DE2
So, KF1
Wu, W1
 
Issue Date2007
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
 
CitationBrain Research, 2007, v. 1159 n. 1, p. 18-27 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.brainres.2007.04.084
 
AbstractIntermediate filaments (IFs) of nestin and vimentin are expressed in immature astrocytes. In this study, we examined the re-expression of these early glial traits in rat reactive astrocytes in affected neuronal perikarya in supraoptic (SON) and paraventricular (PVN) nuclei induced by hypophysectomy. Double-labeling immunofluorescence confocal laser microscopy demonstrated that by 7 days post-lesion, both nestin and vimentin were present intensely in hypertrophied GFAP-IR reactive astrocytes in the area of hypophysectomized magnocellular neurons in SON and PVN, while nestin and vimentin are absent in the normal or sham-operated animals. As the gliotic reaction progressed, the morphology of nestin or vimentin-positive reactive astrocytes in SON but not PVN changed from stellate form at 7 days to thin and elongated shape, morphologically compatible with radial glia during development, at 14 days post-lesion. By 28 days post-lesion, while vimentin-IR persisted in reactive astrocytes in SON and PVN, nestin-IR could hardly be detected. The spatiotemporal pattern of nestin-IR and/or vimentin-IR in reactive astrocytes suggests astrocytes attempt to revert to a more primitive glia form indicated by changes in morphology and phenotype following hypophysectomy, which may contribute to neuronal trophism and plasticity in the lesioned HNS favoring neuronal maintenance and fiber outgrowth. © 2007 Elsevier B.V. All rights reserved.
 
ISSN0006-8993
2012 Impact Factor: 2.879
2012 SCImago Journal Rankings: 1.217
 
DOIhttp://dx.doi.org/10.1016/j.brainres.2007.04.084
 
ISI Accession Number IDWOS:000248614100003
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYuan, Q
 
dc.contributor.authorScott, DE
 
dc.contributor.authorSo, KF
 
dc.contributor.authorWu, W
 
dc.date.accessioned2010-09-06T05:56:25Z
 
dc.date.available2010-09-06T05:56:25Z
 
dc.date.issued2007
 
dc.description.abstractIntermediate filaments (IFs) of nestin and vimentin are expressed in immature astrocytes. In this study, we examined the re-expression of these early glial traits in rat reactive astrocytes in affected neuronal perikarya in supraoptic (SON) and paraventricular (PVN) nuclei induced by hypophysectomy. Double-labeling immunofluorescence confocal laser microscopy demonstrated that by 7 days post-lesion, both nestin and vimentin were present intensely in hypertrophied GFAP-IR reactive astrocytes in the area of hypophysectomized magnocellular neurons in SON and PVN, while nestin and vimentin are absent in the normal or sham-operated animals. As the gliotic reaction progressed, the morphology of nestin or vimentin-positive reactive astrocytes in SON but not PVN changed from stellate form at 7 days to thin and elongated shape, morphologically compatible with radial glia during development, at 14 days post-lesion. By 28 days post-lesion, while vimentin-IR persisted in reactive astrocytes in SON and PVN, nestin-IR could hardly be detected. The spatiotemporal pattern of nestin-IR and/or vimentin-IR in reactive astrocytes suggests astrocytes attempt to revert to a more primitive glia form indicated by changes in morphology and phenotype following hypophysectomy, which may contribute to neuronal trophism and plasticity in the lesioned HNS favoring neuronal maintenance and fiber outgrowth. © 2007 Elsevier B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBrain Research, 2007, v. 1159 n. 1, p. 18-27 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.brainres.2007.04.084
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.brainres.2007.04.084
 
dc.identifier.epage27
 
dc.identifier.hkuros135156
 
dc.identifier.isiWOS:000248614100003
 
dc.identifier.issn0006-8993
2012 Impact Factor: 2.879
2012 SCImago Journal Rankings: 1.217
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid17573051
 
dc.identifier.scopuseid_2-s2.0-34447299935
 
dc.identifier.spage18
 
dc.identifier.urihttp://hdl.handle.net/10722/67582
 
dc.identifier.volume1159
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofBrain Research
 
dc.relation.referencesReferences in Scopus
 
dc.rightsBrain Research. Copyright © Elsevier BV.
 
dc.subject.meshAnimals
 
dc.subject.meshArginine Vasopressin - metabolism
 
dc.subject.meshAstrocytes - classification - metabolism
 
dc.subject.meshBromodeoxyuridine - metabolism
 
dc.subject.meshGene Expression Regulation - physiology
 
dc.subject.meshGlial Fibrillary Acidic Protein - metabolism
 
dc.subject.meshHypophysectomy
 
dc.subject.meshIntermediate Filament Proteins - metabolism
 
dc.subject.meshMale
 
dc.subject.meshNerve Tissue Proteins - metabolism
 
dc.subject.meshNeurons - metabolism
 
dc.subject.meshParaventricular Hypothalamic Nucleus - cytology
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshSupraoptic Nucleus - cytology
 
dc.subject.meshTime Factors
 
dc.subject.meshVimentin - metabolism
 
dc.titleA subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Eastern Virginia Medical School