Article: A subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats

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TitleA subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats
AuthorsYuan, Q1
Scott, DE2
So, KF1
Wu, W1
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
CitationBrain Research, 2007, v. 1159 n. 1, p. 18-27 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.brainres.2007.04.084
AbstractIntermediate filaments (IFs) of nestin and vimentin are expressed in immature astrocytes. In this study, we examined the re-expression of these early glial traits in rat reactive astrocytes in affected neuronal perikarya in supraoptic (SON) and paraventricular (PVN) nuclei induced by hypophysectomy. Double-labeling immunofluorescence confocal laser microscopy demonstrated that by 7 days post-lesion, both nestin and vimentin were present intensely in hypertrophied GFAP-IR reactive astrocytes in the area of hypophysectomized magnocellular neurons in SON and PVN, while nestin and vimentin are absent in the normal or sham-operated animals. As the gliotic reaction progressed, the morphology of nestin or vimentin-positive reactive astrocytes in SON but not PVN changed from stellate form at 7 days to thin and elongated shape, morphologically compatible with radial glia during development, at 14 days post-lesion. By 28 days post-lesion, while vimentin-IR persisted in reactive astrocytes in SON and PVN, nestin-IR could hardly be detected. The spatiotemporal pattern of nestin-IR and/or vimentin-IR in reactive astrocytes suggests astrocytes attempt to revert to a more primitive glia form indicated by changes in morphology and phenotype following hypophysectomy, which may contribute to neuronal trophism and plasticity in the lesioned HNS favoring neuronal maintenance and fiber outgrowth. © 2007 Elsevier B.V. All rights reserved.
ISSN0006-8993
2011 Impact Factor: 2.728
2011 SCImago Journal Rankings: 0.210
DOIhttp://dx.doi.org/10.1016/j.brainres.2007.04.084
ISI Accession Number IDWOS:000248614100003
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorYuan, Q
dc.contributor.authorScott, DE
dc.contributor.authorSo, KF
dc.contributor.authorWu, W
dc.date.accessioned2010-09-06T05:56:25Z
dc.date.available2010-09-06T05:56:25Z
dc.date.issued2007
dc.description.abstractIntermediate filaments (IFs) of nestin and vimentin are expressed in immature astrocytes. In this study, we examined the re-expression of these early glial traits in rat reactive astrocytes in affected neuronal perikarya in supraoptic (SON) and paraventricular (PVN) nuclei induced by hypophysectomy. Double-labeling immunofluorescence confocal laser microscopy demonstrated that by 7 days post-lesion, both nestin and vimentin were present intensely in hypertrophied GFAP-IR reactive astrocytes in the area of hypophysectomized magnocellular neurons in SON and PVN, while nestin and vimentin are absent in the normal or sham-operated animals. As the gliotic reaction progressed, the morphology of nestin or vimentin-positive reactive astrocytes in SON but not PVN changed from stellate form at 7 days to thin and elongated shape, morphologically compatible with radial glia during development, at 14 days post-lesion. By 28 days post-lesion, while vimentin-IR persisted in reactive astrocytes in SON and PVN, nestin-IR could hardly be detected. The spatiotemporal pattern of nestin-IR and/or vimentin-IR in reactive astrocytes suggests astrocytes attempt to revert to a more primitive glia form indicated by changes in morphology and phenotype following hypophysectomy, which may contribute to neuronal trophism and plasticity in the lesioned HNS favoring neuronal maintenance and fiber outgrowth. © 2007 Elsevier B.V. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationBrain Research, 2007, v. 1159 n. 1, p. 18-27 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.brainres.2007.04.084
dc.identifier.doihttp://dx.doi.org/10.1016/j.brainres.2007.04.084
dc.identifier.epage27
dc.identifier.hkuros135156
dc.identifier.isiWOS:000248614100003
dc.identifier.issn0006-8993
2011 Impact Factor: 2.728
2011 SCImago Journal Rankings: 0.210
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid17573051
dc.identifier.scopuseid_2-s2.0-34447299935
dc.identifier.spage18
dc.identifier.urihttp://hdl.handle.net/10722/67582
dc.identifier.volume1159
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
dc.publisher.placeNetherlands
dc.relation.ispartofBrain Research
dc.relation.referencesReferences in Scopus
dc.rightsBrain Research. Copyright © Elsevier BV.
dc.subject.meshAnimals
dc.subject.meshArginine Vasopressin - metabolism
dc.subject.meshAstrocytes - classification - metabolism
dc.subject.meshBromodeoxyuridine - metabolism
dc.subject.meshGene Expression Regulation - physiology
dc.subject.meshGlial Fibrillary Acidic Protein - metabolism
dc.subject.meshHypophysectomy
dc.subject.meshIntermediate Filament Proteins - metabolism
dc.subject.meshMale
dc.subject.meshNerve Tissue Proteins - metabolism
dc.subject.meshNeurons - metabolism
dc.subject.meshParaventricular Hypothalamic Nucleus - cytology
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshSupraoptic Nucleus - cytology
dc.subject.meshTime Factors
dc.subject.meshVimentin - metabolism
dc.titleA subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Eastern Virginia Medical School