Article: In vitro study of regulation of IL-6 production in bronchiectasis

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TitleIn vitro study of regulation of IL-6 production in bronchiectasis
AuthorsHo, JC1
Tipoe, G1
Zheng, L1
Leung, TM1
Tsang, KWT1
Shum, DKY
Lau, CS1
Mak, JCW1
Lam, WK1
Ip, MSM1
KeywordsBronchiectasis
Drug modulation
Interleukin-6
Issue Date2004
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
CitationRespiratory Medicine, 2004, v. 98 n. 4, p. 334-341 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.rmed.2003.10.012
AbstractPersistent airway inflammation is an important pathogenetic factor in bronchiectasis, and interleukin (IL)-6 is among the mediators implicated in regulation of inflammation in bronchiectatic airways. We postulated that airway secretion with its constituents of cytokines and enzymes would provide an environment for perpetuation of inflammation in vivo. We aimed to determine the action of sputum from patients with bronchiectasis on IL-6 production from cultured normal human bronchial epithelial (NHBE) cells and its modulation by anti-inflammatory drugs in vitro. Cultures of NHBE cells were tested with (i) sputum of bronchiectatic patients, (ii) anti-tumor necrosis factor-alpha (TNF-α) pre-treated sputum, or (iii) recombinant human (rh)-TNF-α. Alternatively, NHBE cells were incubated with one of the anti-inflammatory drugs before treatment with sputum or rh-TNF-α. IL-6 produced into the medium was assayed by ELISA. Sputum in bronchiectasis stimulated IL-6 production from NHBE cells by 1.9 times. This was largely attributable to TNF-α as pre-incubation of sputum sol with anti-TNF-α almost neutralized the sputum effect. Apart from dexamethasone, the other drugs exerted inhibitory effects on IL-6 production. Ibuprofen suppressed sputum-stimulated IL-6 production to levels above control and effect levelled off at 50-100μg/ml, contrasting the dose-dependent suppression to control level with MK-663 (0.1-10μg/ml) and to sub-control levels with triptolide (20-1000ng/ml). Our results support that sputum in bronchiectasis can stimulate IL-6 production from NHBE cells, and TNF-α is an important cytokine mediating the process. The suppressive effects observed with ibuprofen, triptolide and MK-663 warrant further study. © 2003 Elsevier Ltd. All rights reserved.
ISSN0954-6111
2011 Impact Factor: 2.475
2011 SCImago Journal Rankings: 0.213
DOIhttp://dx.doi.org/10.1016/j.rmed.2003.10.012
ISI Accession Number IDWOS:000220633100009
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorHo, JC
dc.contributor.authorTipoe, G
dc.contributor.authorZheng, L
dc.contributor.authorLeung, TM
dc.contributor.authorTsang, KWT
dc.contributor.authorShum, DKY
dc.contributor.authorLau, CS
dc.contributor.authorMak, JCW
dc.contributor.authorLam, WK
dc.contributor.authorIp, MSM
dc.date.accessioned2010-09-06T05:56:19Z
dc.date.available2010-09-06T05:56:19Z
dc.date.issued2004
dc.description.abstractPersistent airway inflammation is an important pathogenetic factor in bronchiectasis, and interleukin (IL)-6 is among the mediators implicated in regulation of inflammation in bronchiectatic airways. We postulated that airway secretion with its constituents of cytokines and enzymes would provide an environment for perpetuation of inflammation in vivo. We aimed to determine the action of sputum from patients with bronchiectasis on IL-6 production from cultured normal human bronchial epithelial (NHBE) cells and its modulation by anti-inflammatory drugs in vitro. Cultures of NHBE cells were tested with (i) sputum of bronchiectatic patients, (ii) anti-tumor necrosis factor-alpha (TNF-α) pre-treated sputum, or (iii) recombinant human (rh)-TNF-α. Alternatively, NHBE cells were incubated with one of the anti-inflammatory drugs before treatment with sputum or rh-TNF-α. IL-6 produced into the medium was assayed by ELISA. Sputum in bronchiectasis stimulated IL-6 production from NHBE cells by 1.9 times. This was largely attributable to TNF-α as pre-incubation of sputum sol with anti-TNF-α almost neutralized the sputum effect. Apart from dexamethasone, the other drugs exerted inhibitory effects on IL-6 production. Ibuprofen suppressed sputum-stimulated IL-6 production to levels above control and effect levelled off at 50-100μg/ml, contrasting the dose-dependent suppression to control level with MK-663 (0.1-10μg/ml) and to sub-control levels with triptolide (20-1000ng/ml). Our results support that sputum in bronchiectasis can stimulate IL-6 production from NHBE cells, and TNF-α is an important cytokine mediating the process. The suppressive effects observed with ibuprofen, triptolide and MK-663 warrant further study. © 2003 Elsevier Ltd. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationRespiratory Medicine, 2004, v. 98 n. 4, p. 334-341 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.rmed.2003.10.012
dc.identifier.doihttp://dx.doi.org/10.1016/j.rmed.2003.10.012
dc.identifier.epage341
dc.identifier.hkuros85890
dc.identifier.isiWOS:000220633100009
dc.identifier.issn0954-6111
2011 Impact Factor: 2.475
2011 SCImago Journal Rankings: 0.213
dc.identifier.issue4
dc.identifier.openurl
dc.identifier.pmid15072174
dc.identifier.scopuseid_2-s2.0-11144358561
dc.identifier.spage334
dc.identifier.urihttp://hdl.handle.net/10722/67571
dc.identifier.volume98
dc.languageeng
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
dc.publisher.placeUnited Kingdom
dc.relation.ispartofRespiratory Medicine
dc.relation.referencesReferences in Scopus
dc.subject.meshAnti-Inflammatory Agents - pharmacology
dc.subject.meshBronchiectasis - metabolism
dc.subject.meshCells, Cultured
dc.subject.meshEpithelial Cells
dc.subject.meshInterleukin-6 - biosynthesis
dc.subjectBronchiectasis
dc.subjectDrug modulation
dc.subjectInterleukin-6
dc.titleIn vitro study of regulation of IL-6 production in bronchiectasis
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong