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Article: In vitro study of regulation of IL-6 production in bronchiectasis
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TitleIn vitro study of regulation of IL-6 production in bronchiectasis
 
AuthorsHo, JC1
Tipoe, G1
Zheng, L1
Leung, TM1
Tsang, KWT1
Shum, DKY
Lau, CS1
Mak, JCW1
Lam, WK1
Ip, MSM1
 
KeywordsBronchiectasis
Drug modulation
Interleukin-6
 
Issue Date2004
 
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
 
CitationRespiratory Medicine, 2004, v. 98 n. 4, p. 334-341 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.rmed.2003.10.012
 
AbstractPersistent airway inflammation is an important pathogenetic factor in bronchiectasis, and interleukin (IL)-6 is among the mediators implicated in regulation of inflammation in bronchiectatic airways. We postulated that airway secretion with its constituents of cytokines and enzymes would provide an environment for perpetuation of inflammation in vivo. We aimed to determine the action of sputum from patients with bronchiectasis on IL-6 production from cultured normal human bronchial epithelial (NHBE) cells and its modulation by anti-inflammatory drugs in vitro. Cultures of NHBE cells were tested with (i) sputum of bronchiectatic patients, (ii) anti-tumor necrosis factor-alpha (TNF-α) pre-treated sputum, or (iii) recombinant human (rh)-TNF-α. Alternatively, NHBE cells were incubated with one of the anti-inflammatory drugs before treatment with sputum or rh-TNF-α. IL-6 produced into the medium was assayed by ELISA. Sputum in bronchiectasis stimulated IL-6 production from NHBE cells by 1.9 times. This was largely attributable to TNF-α as pre-incubation of sputum sol with anti-TNF-α almost neutralized the sputum effect. Apart from dexamethasone, the other drugs exerted inhibitory effects on IL-6 production. Ibuprofen suppressed sputum-stimulated IL-6 production to levels above control and effect levelled off at 50-100μg/ml, contrasting the dose-dependent suppression to control level with MK-663 (0.1-10μg/ml) and to sub-control levels with triptolide (20-1000ng/ml). Our results support that sputum in bronchiectasis can stimulate IL-6 production from NHBE cells, and TNF-α is an important cytokine mediating the process. The suppressive effects observed with ibuprofen, triptolide and MK-663 warrant further study. © 2003 Elsevier Ltd. All rights reserved.
 
ISSN0954-6111
2013 Impact Factor: 2.917
 
DOIhttp://dx.doi.org/10.1016/j.rmed.2003.10.012
 
ISI Accession Number IDWOS:000220633100009
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHo, JC
 
dc.contributor.authorTipoe, G
 
dc.contributor.authorZheng, L
 
dc.contributor.authorLeung, TM
 
dc.contributor.authorTsang, KWT
 
dc.contributor.authorShum, DKY
 
dc.contributor.authorLau, CS
 
dc.contributor.authorMak, JCW
 
dc.contributor.authorLam, WK
 
dc.contributor.authorIp, MSM
 
dc.date.accessioned2010-09-06T05:56:19Z
 
dc.date.available2010-09-06T05:56:19Z
 
dc.date.issued2004
 
dc.description.abstractPersistent airway inflammation is an important pathogenetic factor in bronchiectasis, and interleukin (IL)-6 is among the mediators implicated in regulation of inflammation in bronchiectatic airways. We postulated that airway secretion with its constituents of cytokines and enzymes would provide an environment for perpetuation of inflammation in vivo. We aimed to determine the action of sputum from patients with bronchiectasis on IL-6 production from cultured normal human bronchial epithelial (NHBE) cells and its modulation by anti-inflammatory drugs in vitro. Cultures of NHBE cells were tested with (i) sputum of bronchiectatic patients, (ii) anti-tumor necrosis factor-alpha (TNF-α) pre-treated sputum, or (iii) recombinant human (rh)-TNF-α. Alternatively, NHBE cells were incubated with one of the anti-inflammatory drugs before treatment with sputum or rh-TNF-α. IL-6 produced into the medium was assayed by ELISA. Sputum in bronchiectasis stimulated IL-6 production from NHBE cells by 1.9 times. This was largely attributable to TNF-α as pre-incubation of sputum sol with anti-TNF-α almost neutralized the sputum effect. Apart from dexamethasone, the other drugs exerted inhibitory effects on IL-6 production. Ibuprofen suppressed sputum-stimulated IL-6 production to levels above control and effect levelled off at 50-100μg/ml, contrasting the dose-dependent suppression to control level with MK-663 (0.1-10μg/ml) and to sub-control levels with triptolide (20-1000ng/ml). Our results support that sputum in bronchiectasis can stimulate IL-6 production from NHBE cells, and TNF-α is an important cytokine mediating the process. The suppressive effects observed with ibuprofen, triptolide and MK-663 warrant further study. © 2003 Elsevier Ltd. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationRespiratory Medicine, 2004, v. 98 n. 4, p. 334-341 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.rmed.2003.10.012
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.rmed.2003.10.012
 
dc.identifier.epage341
 
dc.identifier.hkuros85890
 
dc.identifier.isiWOS:000220633100009
 
dc.identifier.issn0954-6111
2013 Impact Factor: 2.917
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid15072174
 
dc.identifier.scopuseid_2-s2.0-11144358561
 
dc.identifier.spage334
 
dc.identifier.urihttp://hdl.handle.net/10722/67571
 
dc.identifier.volume98
 
dc.languageeng
 
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofRespiratory Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnti-Inflammatory Agents - pharmacology
 
dc.subject.meshBronchiectasis - metabolism
 
dc.subject.meshCells, Cultured
 
dc.subject.meshEpithelial Cells
 
dc.subject.meshInterleukin-6 - biosynthesis
 
dc.subjectBronchiectasis
 
dc.subjectDrug modulation
 
dc.subjectInterleukin-6
 
dc.titleIn vitro study of regulation of IL-6 production in bronchiectasis
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong