File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Up-regulation of TRPM-2, MMP-7 and ID-1 during sex hormone-induced prostate carcinogenesis in the Noble rat

TitleUp-regulation of TRPM-2, MMP-7 and ID-1 during sex hormone-induced prostate carcinogenesis in the Noble rat
Authors
Issue Date2001
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
Citation
Carcinogenesis, 2001, v. 22 n. 6, p. 965-973 How to Cite?
AbstractProstate cancer is the most frequently diagnosed malignancy in the Western world and changes in the ratio of testosterone and estrogens with advancing age is one of the potential risk factors in the development of this disease. However, the molecular mechanisms associated with hormone imbalance in prostate carcinogenesis are poorly understood. In this study we induced a high incidence of prostate hyperplasia, dysplasia and adenocarcinoma in the Noble rat using a combination of testosterone and estradiol-17β. Using this animal model, we studied the gene expression profile during sex hormone-induced prostate carcinogenesis using a cDNA array technique; the results were further confirmed by RT-PCR, western blotting and immunohistochemical analyses. We found up-regulation of TRPM-2 (testosterone-repressed prostatic message-2), MMP-7 (matrix metalloproteinase-7) and Id-1 (inhibitor of differentiation or DNA binding) during development of sex hormone-induced prostate cancer. Increased expression of TRPM-2 and MMP-7 was observed in both premalignant and malignant tissues after sex hormone treatment, indicating their role in the early stages of hormone response and prostate cancer development. In contrast, Id-1 was expressed at relatively low levels in all premalignant samples but increased in malignant cells, suggesting its potential roles as a biomarker for prostate cancer cells. Furthermore, expression of Id-1 appeared to be stronger in poorly differentiated lesions than in well-differentiated carcinomas, suggesting that the levels of Id-1 expression may be correlated with the malignancy of tumors. Our results provide the first evidence of up-regulation of TRPM-2, MMP-7 and Id-1 during sex hormone-induced prostate carcinogenesis and strongly suggest their association with the development of prostate cancer.
Persistent Identifierhttp://hdl.handle.net/10722/67556
ISSN
2015 Impact Factor: 4.874
2015 SCImago Journal Rankings: 2.439
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorOuyang, XSen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorLee, DTWen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorWong, YCen_HK
dc.date.accessioned2010-09-06T05:56:11Z-
dc.date.available2010-09-06T05:56:11Z-
dc.date.issued2001en_HK
dc.identifier.citationCarcinogenesis, 2001, v. 22 n. 6, p. 965-973en_HK
dc.identifier.issn0143-3334en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67556-
dc.description.abstractProstate cancer is the most frequently diagnosed malignancy in the Western world and changes in the ratio of testosterone and estrogens with advancing age is one of the potential risk factors in the development of this disease. However, the molecular mechanisms associated with hormone imbalance in prostate carcinogenesis are poorly understood. In this study we induced a high incidence of prostate hyperplasia, dysplasia and adenocarcinoma in the Noble rat using a combination of testosterone and estradiol-17β. Using this animal model, we studied the gene expression profile during sex hormone-induced prostate carcinogenesis using a cDNA array technique; the results were further confirmed by RT-PCR, western blotting and immunohistochemical analyses. We found up-regulation of TRPM-2 (testosterone-repressed prostatic message-2), MMP-7 (matrix metalloproteinase-7) and Id-1 (inhibitor of differentiation or DNA binding) during development of sex hormone-induced prostate cancer. Increased expression of TRPM-2 and MMP-7 was observed in both premalignant and malignant tissues after sex hormone treatment, indicating their role in the early stages of hormone response and prostate cancer development. In contrast, Id-1 was expressed at relatively low levels in all premalignant samples but increased in malignant cells, suggesting its potential roles as a biomarker for prostate cancer cells. Furthermore, expression of Id-1 appeared to be stronger in poorly differentiated lesions than in well-differentiated carcinomas, suggesting that the levels of Id-1 expression may be correlated with the malignancy of tumors. Our results provide the first evidence of up-regulation of TRPM-2, MMP-7 and Id-1 during sex hormone-induced prostate carcinogenesis and strongly suggest their association with the development of prostate cancer.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/en_HK
dc.relation.ispartofCarcinogenesisen_HK
dc.rightsCarcinogenesis. Copyright © Oxford University Press.en_HK
dc.subject.meshAdenocarcinoma - chemically induced - genetics - metabolism - pathologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshClusterinen_HK
dc.subject.meshDNA-Binding Proteins - biosynthesis - geneticsen_HK
dc.subject.meshEstradiol - toxicityen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGlycoproteins - biosynthesis - geneticsen_HK
dc.subject.meshInhibitor of Differentiation Protein 1en_HK
dc.subject.meshMaleen_HK
dc.subject.meshMatrix Metalloproteinase 7 - biosynthesis - geneticsen_HK
dc.subject.meshMolecular Chaperones - biosynthesis - geneticsen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshProstate - drug effects - pathologyen_HK
dc.subject.meshProstatic Hyperplasia - chemically induced - genetics - metabolism - pathologyen_HK
dc.subject.meshProstatic Neoplasms - chemically induced - genetics - metabolism - pathologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRepressor Proteinsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTestosterone - toxicityen_HK
dc.subject.meshTranscription Factors - biosynthesis - geneticsen_HK
dc.subject.meshUp-Regulationen_HK
dc.titleUp-regulation of TRPM-2, MMP-7 and ID-1 during sex hormone-induced prostate carcinogenesis in the Noble raten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0143-3334&volume=22 No 6&spage=965&epage=973&date=2001&atitle=Up-regulation+of+TRPM-2,+MMP-7+and+ID-1+during+sex+hormone-induced+prostate+carcinogenesis+in+the+Noble+raten_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid11375906-
dc.identifier.scopuseid_2-s2.0-0034986503en_HK
dc.identifier.hkuros57324en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034986503&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue6en_HK
dc.identifier.spage965en_HK
dc.identifier.epage973en_HK
dc.identifier.isiWOS:000169171500019-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridOuyang, XS=8711278300en_HK
dc.identifier.scopusauthoridWang, X=7501854829en_HK
dc.identifier.scopusauthoridLee, DTW=7406666118en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats