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Article: Specific expression of VCY2 in human male germ cells and its involvement in the pathogenesis of male infertility

TitleSpecific expression of VCY2 in human male germ cells and its involvement in the pathogenesis of male infertility
Authors
KeywordsSperm
Spermatogenesis
Testis
Issue Date2003
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2003, v. 69 n. 3, p. 746-751 How to Cite?
AbstractAbnormal spermatogenesis in men with Y-chromosome microdeletions suggests that genes important for spermatogenesis have been removed from these individuals. VCY2 is a testis-specific gene that locates in the most frequently deleted azoospermia factor c region in the Y chromosome. We have raised an antiserum to VCY2 and used it to characterize the localization of VCY2 in human testis. Using Western blot analysis, the affinity-purified polyclonal VCY2 antibody gave a single specific band of approximately 14 kDa in size, corresponding to the expected size of VCY2 in all the collected human testicular biopsy specimens with normal spermatogenesis. Immunohistochemical analyses showed that VCY2 localized to the nuclei of spermatogonia, spermatocytes, and round spermatids, except elongated spermatids. At the ultrastructural level, VCY2 expression was found in the nucleus of human ejaculated spermatozoa. To determine the possible relationship of VCY2 with the pathogenesis of male infertility, we examined a group of infertile men with and without Y-chromosome microdeletions and with known testicular pathology using VCY2 antibody. VCY2 was weakly expressed at the spermatogonia and immunonegative in spermatocytes and round spermatids in testicular biopsy specimens with maturation arrest or hypospermatogenesis. The specific localization of the protein in germ cell nuclei indicates that VCY2 is likely to function in male germ cell development. The impaired expression of VCY2 in infertile men suggests its involvement in the pathogenesis of male infertility.
Persistent Identifierhttp://hdl.handle.net/10722/67553
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, JYMen_HK
dc.contributor.authorWong, EYMen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorTam, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T05:56:09Z-
dc.date.available2010-09-06T05:56:09Z-
dc.date.issued2003en_HK
dc.identifier.citationBiology Of Reproduction, 2003, v. 69 n. 3, p. 746-751en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67553-
dc.description.abstractAbnormal spermatogenesis in men with Y-chromosome microdeletions suggests that genes important for spermatogenesis have been removed from these individuals. VCY2 is a testis-specific gene that locates in the most frequently deleted azoospermia factor c region in the Y chromosome. We have raised an antiserum to VCY2 and used it to characterize the localization of VCY2 in human testis. Using Western blot analysis, the affinity-purified polyclonal VCY2 antibody gave a single specific band of approximately 14 kDa in size, corresponding to the expected size of VCY2 in all the collected human testicular biopsy specimens with normal spermatogenesis. Immunohistochemical analyses showed that VCY2 localized to the nuclei of spermatogonia, spermatocytes, and round spermatids, except elongated spermatids. At the ultrastructural level, VCY2 expression was found in the nucleus of human ejaculated spermatozoa. To determine the possible relationship of VCY2 with the pathogenesis of male infertility, we examined a group of infertile men with and without Y-chromosome microdeletions and with known testicular pathology using VCY2 antibody. VCY2 was weakly expressed at the spermatogonia and immunonegative in spermatocytes and round spermatids in testicular biopsy specimens with maturation arrest or hypospermatogenesis. The specific localization of the protein in germ cell nuclei indicates that VCY2 is likely to function in male germ cell development. The impaired expression of VCY2 in infertile men suggests its involvement in the pathogenesis of male infertility.en_HK
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.subjectSpermen_HK
dc.subjectSpermatogenesisen_HK
dc.subjectTestisen_HK
dc.subject.meshAdulten_HK
dc.subject.meshBiopsyen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Nucleusen_HK
dc.subject.meshChromosome Deletionen_HK
dc.subject.meshChromosomes, Human, Y - genetics - metabolism - ultrastructureen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshInfertility, Male - genetics - metabolism - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshProteins - genetics - metabolismen_HK
dc.subject.meshRNA, Messenger - analysisen_HK
dc.subject.meshSpermatogenesis - physiologyen_HK
dc.subject.meshSpermatozoa - metabolism - pathology - ultrastructureen_HK
dc.subject.meshTestis - metabolism - pathologyen_HK
dc.titleSpecific expression of VCY2 in human male germ cells and its involvement in the pathogenesis of male infertilityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3363&volume=69&spage=746&epage=751&date=2003&atitle=Specific+expression+of+VCY2+in+human+male+germ+cells+and+its+involvement+in+the+pathogenesis+of+male+infertility+en_HK
dc.identifier.emailWong, EYM: elainewg@hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityWong, EYM=rp01718en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1095/biolreprod.103.015792en_HK
dc.identifier.pmid12724276-
dc.identifier.scopuseid_2-s2.0-0041914418en_HK
dc.identifier.hkuros88133en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0041914418&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume69en_HK
dc.identifier.issue3en_HK
dc.identifier.spage746en_HK
dc.identifier.epage751en_HK
dc.identifier.isiWOS:000184989100002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTse, JYM=7102607314en_HK
dc.identifier.scopusauthoridWong, EYM=36719382700en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.scopusauthoridTam, PC=7202539419en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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