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Article: The relationship between vascularity and cell proliferation in human normal and pathological lesions of the oral cheek epithelium

TitleThe relationship between vascularity and cell proliferation in human normal and pathological lesions of the oral cheek epithelium
Authors
Issue Date1996
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/oraloncology
Citation
European Journal Of Cancer Part B: Oral Oncology, 1996, v. 32 n. 1, p. 24-31 How to Cite?
AbstractThe present study investigates relationships between neovascularisation and PCNA cell proliferation markers in different pathological lesions of the oral cheek mucosa. All specimens were fixed in 10% formalin and routinely processed for histology. Six normal (N) samples were taken from resection margins of benign lesions. The pathological lesions consisted of chronic inflammation (n = 10), lichen planus (n = 7), fibrous hyperplasia (n = 11), dysplasia (n = 5), squamous cell carcinoma (n = 22) and epithelium adjacent to carcinoma (n = 6). Adjacent 5 μm sections were stained with monoclonal antibodies against vimentin (clone no. V9) for identification of stromal blood vessels and against proliferating nuclear antigen (PCNA/PC10) using ABC immunoperoxidase techniques. Point counting was used to obtain the primary morphometric data using a Zeiss VIDAS image analyser. No attempt was made to classify the different types of blood vessels. The morphometric blood vessel parameters estimated were volume density, number per unit area, length per unit volume and mean transverse sectional area. PCNA indices were determined by estimating the percentage frequency of PCNA positive nuclei in basal and spinous strata. Generally, there were significant increases in all PCNA indices and blood vessel parameters between the N group and the different pathological lesions. A highly positive correlation was detected between all PCNA indices and blood vessel parameters. These data suggest that increased vascularity and angiogenesis occur in support of actively proliferating and transforming oral epithelial cells in order to permit growth. PCNA indices and blood vessel parameters may have a potential application as diagnostic and prognostic indicators.
Persistent Identifierhttp://hdl.handle.net/10722/67547
ISSN
2002 Impact Factor: 1.873
2000 SCImago Journal Rankings: 0.448
References

 

DC FieldValueLanguage
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorJin, Yen_HK
dc.contributor.authorWhite, FHen_HK
dc.date.accessioned2010-09-06T05:56:06Z-
dc.date.available2010-09-06T05:56:06Z-
dc.date.issued1996en_HK
dc.identifier.citationEuropean Journal Of Cancer Part B: Oral Oncology, 1996, v. 32 n. 1, p. 24-31en_HK
dc.identifier.issn0964-1955en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67547-
dc.description.abstractThe present study investigates relationships between neovascularisation and PCNA cell proliferation markers in different pathological lesions of the oral cheek mucosa. All specimens were fixed in 10% formalin and routinely processed for histology. Six normal (N) samples were taken from resection margins of benign lesions. The pathological lesions consisted of chronic inflammation (n = 10), lichen planus (n = 7), fibrous hyperplasia (n = 11), dysplasia (n = 5), squamous cell carcinoma (n = 22) and epithelium adjacent to carcinoma (n = 6). Adjacent 5 μm sections were stained with monoclonal antibodies against vimentin (clone no. V9) for identification of stromal blood vessels and against proliferating nuclear antigen (PCNA/PC10) using ABC immunoperoxidase techniques. Point counting was used to obtain the primary morphometric data using a Zeiss VIDAS image analyser. No attempt was made to classify the different types of blood vessels. The morphometric blood vessel parameters estimated were volume density, number per unit area, length per unit volume and mean transverse sectional area. PCNA indices were determined by estimating the percentage frequency of PCNA positive nuclei in basal and spinous strata. Generally, there were significant increases in all PCNA indices and blood vessel parameters between the N group and the different pathological lesions. A highly positive correlation was detected between all PCNA indices and blood vessel parameters. These data suggest that increased vascularity and angiogenesis occur in support of actively proliferating and transforming oral epithelial cells in order to permit growth. PCNA indices and blood vessel parameters may have a potential application as diagnostic and prognostic indicators.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/oraloncologyen_HK
dc.relation.ispartofEuropean Journal of Cancer Part B: Oral Oncologyen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshCell Transformation, Neoplastic - pathologyen_HK
dc.subject.meshCheeken_HK
dc.subject.meshEpithelial Cellsen_HK
dc.subject.meshEpithelium - blood supply - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoenzyme Techniquesen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMouth Mucosa - blood supply - cytology - pathologyen_HK
dc.subject.meshMouth Neoplasms - pathologyen_HK
dc.subject.meshNeovascularization, Pathologic - pathologyen_HK
dc.subject.meshPrecancerous Conditions - pathologyen_HK
dc.subject.meshProliferating Cell Nuclear Antigen - analysisen_HK
dc.titleThe relationship between vascularity and cell proliferation in human normal and pathological lesions of the oral cheek epitheliumen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1368-8375&volume=32B&spage=24&epage=31&date=1996&atitle=The+relationship+between+vascularity+and+cell+proliferation+in+human+normal+and+pathological+lesions+of+the+oral+cheek+epitheliumen_HK
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0964-1955(95)00049-6en_HK
dc.identifier.pmid8729615en_HK
dc.identifier.scopuseid_2-s2.0-0029920759en_HK
dc.identifier.hkuros10121en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029920759&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue1en_HK
dc.identifier.spage24en_HK
dc.identifier.epage31en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.scopusauthoridJin, Y=26643271200en_HK
dc.identifier.scopusauthoridWhite, FH=7202578907en_HK

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