File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Molecular cloning of differentially expressed genes in human epithelial ovarian cancer

TitleMolecular cloning of differentially expressed genes in human epithelial ovarian cancer
Authors
Issue Date1994
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 1994, v. 52 n. 2, p. 247-252 How to Cite?
AbstractA DNA-fingerprinting approach has been adapted to detect differentially expressed genes in human ovarian carcinoma. This method is based on the use of arbitrary primers to generate fingerprints from total RNA isolated from normal ovarian epithelial cells and ovarian carcinoma cells by polymerase chain reaction (PCR). Using this method, we cloned two cDNA fragments (DOC-1 and DOC-2) which were present in normal ovarian surface epithelial cells but consistently absent in all of the ovarian cancer cell lines from the differential display. In addition, we also identified a cDNA fragment (LF4.0) which is overexpressed in most of the tumor cell lines and tumor tissues in comparison to the normal ovarian surface epithelial cells. The differential expression of the genes in the tumor cell lines as well as in the tumor tissues was also confirmed by Northern analysis. The clone DOC-2, which is a 800-bp cDNA fragment, has one open reading frame suggesting that the gene may be translated. Assuming that this frame is the sense strand, we generated both sense and antisense riboprobe for in situ mRNA hybridization. Only the antisense DOC-2 riboprobe revealed a hybridization signal which was restricted to the human surface ovarian epithelium. The potential functional roles of these genes is now under investigation.
Persistent Identifierhttp://hdl.handle.net/10722/67537
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMok, SCen_HK
dc.contributor.authorWong, KKen_HK
dc.contributor.authorChan, RKWen_HK
dc.contributor.authorLau, CCen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorKnapp, RCen_HK
dc.contributor.authorBerkowitz, RSen_HK
dc.date.accessioned2010-09-06T05:56:01Z-
dc.date.available2010-09-06T05:56:01Z-
dc.date.issued1994en_HK
dc.identifier.citationGynecologic Oncology, 1994, v. 52 n. 2, p. 247-252en_HK
dc.identifier.issn0090-8258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67537-
dc.description.abstractA DNA-fingerprinting approach has been adapted to detect differentially expressed genes in human ovarian carcinoma. This method is based on the use of arbitrary primers to generate fingerprints from total RNA isolated from normal ovarian epithelial cells and ovarian carcinoma cells by polymerase chain reaction (PCR). Using this method, we cloned two cDNA fragments (DOC-1 and DOC-2) which were present in normal ovarian surface epithelial cells but consistently absent in all of the ovarian cancer cell lines from the differential display. In addition, we also identified a cDNA fragment (LF4.0) which is overexpressed in most of the tumor cell lines and tumor tissues in comparison to the normal ovarian surface epithelial cells. The differential expression of the genes in the tumor cell lines as well as in the tumor tissues was also confirmed by Northern analysis. The clone DOC-2, which is a 800-bp cDNA fragment, has one open reading frame suggesting that the gene may be translated. Assuming that this frame is the sense strand, we generated both sense and antisense riboprobe for in situ mRNA hybridization. Only the antisense DOC-2 riboprobe revealed a hybridization signal which was restricted to the human surface ovarian epithelium. The potential functional roles of these genes is now under investigation.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_HK
dc.relation.ispartofGynecologic Oncologyen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBlotting, Northernen_HK
dc.subject.meshCarcinoma - genetics - pathologyen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshDNA Fingerprintingen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshMolecular Probes - geneticsen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshOvarian Neoplasms - genetics - pathologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleMolecular cloning of differentially expressed genes in human epithelial ovarian canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0090-8258&volume=52&spage=247&epage=252&date=1994&atitle=Molecular+cloning+of+differentially+expressed+genes+in+human+epithelial+ovarian+canceren_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/gyno.1994.1040en_HK
dc.identifier.pmid8314147-
dc.identifier.scopuseid_2-s2.0-0028031863en_HK
dc.identifier.hkuros4702en_HK
dc.identifier.volume52en_HK
dc.identifier.issue2en_HK
dc.identifier.spage247en_HK
dc.identifier.epage252en_HK
dc.identifier.isiWOS:A1994MX03700019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMok, SC=7006015487en_HK
dc.identifier.scopusauthoridWong, KK=37096551700en_HK
dc.identifier.scopusauthoridChan, RKW=36907988700en_HK
dc.identifier.scopusauthoridLau, CC=7401968381en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridKnapp, RC=7201853287en_HK
dc.identifier.scopusauthoridBerkowitz, RS=7201352221en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats