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Article: Melanopsin-expressing retinal ganglion cells are more injury-resistant in a chronic ocular hypertension model

TitleMelanopsin-expressing retinal ganglion cells are more injury-resistant in a chronic ocular hypertension model
Authors
Issue Date2006
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2006, v. 47 n. 7, p. 2951-2958 How to Cite?
AbstractPURPOSE. To investigate the survival of melanopsin-expressing retinal ganglion cells (mRGCs) after the induction of chronic ocular hypertension. METHODS. Intraocular pressure (IOP) was elevated in adult Sprague-Dawley rats using an argon laser to photocoagulate the episcleral and limbal veins. IOP was measured with a calibrated tonometer and monitored for a period. Seven days before the animals were killed, a piece of sterile foam soaked with gold fluorescent dye was placed onto the superior colliculus (SC) to label the SC-projecting retinal ganglion cells (scRGCs) retrogradely. mRGCs were visualized by free floating immunohistochemistry on whole-mounted retinas. The number of surviving scRGCs and mRGCs were counted on flatmounted retinas. The branching pattern of dendrites and soma size of mRGCs were examined. RESULTS. An ̃1.7-fold increase of IOP and a significant loss of scRGCs were found in experimental eyes after laser photocoagulation. However, no significant cell loss or morphologic changes on mRGCs and their dendrites after the induction of chronic ocular hypertension are noticed over a 12-week period. CONCLUSIONS. Although the degeneration of retinal ganglion cells (RGCs) is a major concern in glaucomatous damage, the findings show that mRGCs are less susceptible to death after the induction of chronic ocular hypertension. This result indicates that mRGCs carry some unique properties that are different from those of other subpopulations of RGCs. The immunohistochemistry approach can be used to distinguish easily these mRGCs from other subtypes. This method provides a useful tool to investigate their injury-resistant properties that are informative for the development of effective neuroprotective treatment for glaucoma. Copyright © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/67520
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, RSen_HK
dc.contributor.authorChen, BYen_HK
dc.contributor.authorTay, DKen_HK
dc.contributor.authorChan, HHLen_HK
dc.contributor.authorPu, MLen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-06T05:55:52Z-
dc.date.available2010-09-06T05:55:52Z-
dc.date.issued2006en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2006, v. 47 n. 7, p. 2951-2958en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67520-
dc.description.abstractPURPOSE. To investigate the survival of melanopsin-expressing retinal ganglion cells (mRGCs) after the induction of chronic ocular hypertension. METHODS. Intraocular pressure (IOP) was elevated in adult Sprague-Dawley rats using an argon laser to photocoagulate the episcleral and limbal veins. IOP was measured with a calibrated tonometer and monitored for a period. Seven days before the animals were killed, a piece of sterile foam soaked with gold fluorescent dye was placed onto the superior colliculus (SC) to label the SC-projecting retinal ganglion cells (scRGCs) retrogradely. mRGCs were visualized by free floating immunohistochemistry on whole-mounted retinas. The number of surviving scRGCs and mRGCs were counted on flatmounted retinas. The branching pattern of dendrites and soma size of mRGCs were examined. RESULTS. An ̃1.7-fold increase of IOP and a significant loss of scRGCs were found in experimental eyes after laser photocoagulation. However, no significant cell loss or morphologic changes on mRGCs and their dendrites after the induction of chronic ocular hypertension are noticed over a 12-week period. CONCLUSIONS. Although the degeneration of retinal ganglion cells (RGCs) is a major concern in glaucomatous damage, the findings show that mRGCs are less susceptible to death after the induction of chronic ocular hypertension. This result indicates that mRGCs carry some unique properties that are different from those of other subpopulations of RGCs. The immunohistochemistry approach can be used to distinguish easily these mRGCs from other subtypes. This method provides a useful tool to investigate their injury-resistant properties that are informative for the development of effective neuroprotective treatment for glaucoma. Copyright © Association for Research in Vision and Ophthalmology.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Counten_HK
dc.subject.meshCell Survival - physiologyen_HK
dc.subject.meshChronic Diseaseen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshIntraocular Pressureen_HK
dc.subject.meshOcular Hypertension - pathologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshRetinal Ganglion Cells - cytology - metabolismen_HK
dc.subject.meshRod Opsins - metabolismen_HK
dc.titleMelanopsin-expressing retinal ganglion cells are more injury-resistant in a chronic ocular hypertension modelen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0146-0404&volume=47&issue=7&spage=2951&epage=2958&date=2006&atitle=Melanopsin-expressing+retinal+ganglion+cells+are+more+injury-resistant+in+a+chronic+ocular+hypertension+modelen_HK
dc.identifier.emailTay, DK:dkctay@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityTay, DK=rp00336en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1167/iovs.05-1295en_HK
dc.identifier.pmid16799038en_HK
dc.identifier.scopuseid_2-s2.0-33746769946en_HK
dc.identifier.hkuros125775en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746769946&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2951en_HK
dc.identifier.epage2958en_HK
dc.identifier.isiWOS:000238688600030-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, RS=14056111900en_HK
dc.identifier.scopusauthoridChen, BY=14051424300en_HK
dc.identifier.scopusauthoridTay, DK=7006796825en_HK
dc.identifier.scopusauthoridChan, HHL=24774420300en_HK
dc.identifier.scopusauthoridPu, ML=16073321400en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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