File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A New Method for Improving Metaphase Chromosome Spreading

TitleA New Method for Improving Metaphase Chromosome Spreading
Authors
KeywordsChromosome spreading
Dynamics
Mechanism
New method
Issue Date2003
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0196-4763/
Citation
Cytometry Part A, 2003, v. 51 n. 1, p. 46-51 How to Cite?
AbstractBackground: The success of complex molecular cytogenetic studies depends on having properly spread chromosomes. However, inconsistency of optimum chromosome spreading remains a major problem in cytogenetic studies. Methods: The metaphase spreading process was carefully timed to identify the most critical phase of chromosome spreading. The effects of dropping height of cell suspension, slide condition, drying time, fixative ratio, and relative humidity on the quality of metaphase spreads were studied by quantitative examination of metaphase chromosome spreads. Normal and immortalized human epithelial ovarian cells, neuroblastoma cells, and normal lymphocytes were tested. Results: Humidity over the slide was the most important variable affecting the quality of chromosome spreads. Consistent improvement in chromosome spreading (larger metaphase area, less chromosome overlaps, or lower frequencies of broken metaphases) was obtained for all cell types if dynamic cell rehydration, occurring as fixative absorbs moisture from air, was made to coincide with the prompt fixation of spread chromosomes to the slide. This was achieved by dropping cells on dry glass slides placed in a shallow metal tray and then quickly lowering the tray into a covered 50°C water bath for slide drying. Conclusions: A new and simple method for improving metaphase chromosome spreading was developed based on our study on the characteristics of chromosome spreading. © 2002 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/67519
ISSN
2015 Impact Factor: 3.181
2015 SCImago Journal Rankings: 1.630
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDeng, Wen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLucas, JNen_HK
dc.contributor.authorLeung, CSen_HK
dc.contributor.authorCheung, ALMen_HK
dc.date.accessioned2010-09-06T05:55:51Z-
dc.date.available2010-09-06T05:55:51Z-
dc.date.issued2003en_HK
dc.identifier.citationCytometry Part A, 2003, v. 51 n. 1, p. 46-51en_HK
dc.identifier.issn1552-4922en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67519-
dc.description.abstractBackground: The success of complex molecular cytogenetic studies depends on having properly spread chromosomes. However, inconsistency of optimum chromosome spreading remains a major problem in cytogenetic studies. Methods: The metaphase spreading process was carefully timed to identify the most critical phase of chromosome spreading. The effects of dropping height of cell suspension, slide condition, drying time, fixative ratio, and relative humidity on the quality of metaphase spreads were studied by quantitative examination of metaphase chromosome spreads. Normal and immortalized human epithelial ovarian cells, neuroblastoma cells, and normal lymphocytes were tested. Results: Humidity over the slide was the most important variable affecting the quality of chromosome spreads. Consistent improvement in chromosome spreading (larger metaphase area, less chromosome overlaps, or lower frequencies of broken metaphases) was obtained for all cell types if dynamic cell rehydration, occurring as fixative absorbs moisture from air, was made to coincide with the prompt fixation of spread chromosomes to the slide. This was achieved by dropping cells on dry glass slides placed in a shallow metal tray and then quickly lowering the tray into a covered 50°C water bath for slide drying. Conclusions: A new and simple method for improving metaphase chromosome spreading was developed based on our study on the characteristics of chromosome spreading. © 2002 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0196-4763/en_HK
dc.relation.ispartofCytometry Part Aen_HK
dc.subjectChromosome spreadingen_HK
dc.subjectDynamicsen_HK
dc.subjectMechanismen_HK
dc.subjectNew methoden_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromosomes - genetics - ultrastructureen_HK
dc.subject.meshCytogenetic Analysis - instrumentation - methodsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHumidity - adverse effectsen_HK
dc.subject.meshMetaphase - geneticsen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.subject.meshTemperatureen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshTissue Fixation - methodsen_HK
dc.titleA New Method for Improving Metaphase Chromosome Spreadingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0196-4763&volume=51A&spage=46&epage=51&date=2003&atitle=A+New+Method+for+Improving+Metaphase+Chromosome+Spreadingen_HK
dc.identifier.emailDeng, W: wdeng@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.emailCheung, ALM: lmcheung@hku.hken_HK
dc.identifier.authorityDeng, W=rp01640en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityCheung, ALM=rp00332en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid12500304en_HK
dc.identifier.scopuseid_2-s2.0-0345168793en_HK
dc.identifier.hkuros75683en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0345168793&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume51en_HK
dc.identifier.issue1en_HK
dc.identifier.spage46en_HK
dc.identifier.epage51en_HK
dc.identifier.isiWOS:000182425600006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDeng, W=7202223673en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLucas, JN=7402441937en_HK
dc.identifier.scopusauthoridLeung, CS=36725510000en_HK
dc.identifier.scopusauthoridCheung, ALM=7401806497en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats