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Article: Regulation of Chromatin Architecture by the PWWP Domain-Containing DNA Damage-Responsive Factor EXPAND1/MUM1

TitleRegulation of Chromatin Architecture by the PWWP Domain-Containing DNA Damage-Responsive Factor EXPAND1/MUM1
Authors
KeywordsDNA
Issue Date2010
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/molcel
Citation
Molecular Cell, 2010, v. 37 n. 6, p. 854-864 How to Cite?
Abstract
Dynamic changes of chromatin structure facilitate diverse biological events, including DNA replication, repair, recombination, and gene transcription. Recent evidence revealed that DNA damage elicits alterations to the chromatin to facilitate proper checkpoint activation and DNA repair. Here we report the identification of the PWWP domain-containing protein EXPAND1/MUM1 as an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. Depletion of EXPAND1/MUM1 or inactivation of its PWWP domain resulted in chromatin compaction. Upon DNA damage, EXPAND1/MUM1 rapidly concentrates at the vicinity of DNA damage sites via its direct interaction with 53BP1. Ablation of this interaction impaired damage-induced chromatin decondensation, which is accompanied by sustained DNA damage and hypersensitivity to genotoxic stress. Collectively, our study uncovers a chromatin-bound factor that serves an accessory role in coupling damage signaling with chromatin changes in response to DNA damage. © 2010 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67495
ISSN
2013 Impact Factor: 14.464
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of HealthCA089239
CA092312
CA100109
Department of Anatomy, HKU
HKU200908159008
Department of Defense
Funding Information:

This work was supported in part by grants from the National Institutes of Health (CA089239, CA092312, and CA100109 to J.C.), from Startup Fund (Department of Anatomy, HKU to M.S.Y.H.) and Seed Funding for Basic Research (Project Code 200908159008; HKU to M.S.Y.H.). M.S.Y.H. would like to thank Drs. Zhiwei Chen and Henggui Liu (AIDS Institute, HKU) for help with flow cytometry analysis and is grateful to J.C. for his continuous support and mentoring. J.C is a recipient of an Era of Hope Scholar award from the Department of Defense and is a member of the Mayo Clinic Breast SPORE Program (P50 CA116201).

References

 

Author Affiliations
  1. The University of Hong Kong
  2. University of Texas M. D. Anderson Cancer Center
DC FieldValueLanguage
dc.contributor.authorHuen, MSYen_HK
dc.contributor.authorHuang, Jen_HK
dc.contributor.authorLeung, JWCen_HK
dc.contributor.authorSy, SMHen_HK
dc.contributor.authorLeung, KMen_HK
dc.contributor.authorChing, YPen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorChen, Jen_HK
dc.date.accessioned2010-09-06T05:55:38Z-
dc.date.available2010-09-06T05:55:38Z-
dc.date.issued2010en_HK
dc.identifier.citationMolecular Cell, 2010, v. 37 n. 6, p. 854-864en_HK
dc.identifier.issn1097-2765en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67495-
dc.description.abstractDynamic changes of chromatin structure facilitate diverse biological events, including DNA replication, repair, recombination, and gene transcription. Recent evidence revealed that DNA damage elicits alterations to the chromatin to facilitate proper checkpoint activation and DNA repair. Here we report the identification of the PWWP domain-containing protein EXPAND1/MUM1 as an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. Depletion of EXPAND1/MUM1 or inactivation of its PWWP domain resulted in chromatin compaction. Upon DNA damage, EXPAND1/MUM1 rapidly concentrates at the vicinity of DNA damage sites via its direct interaction with 53BP1. Ablation of this interaction impaired damage-induced chromatin decondensation, which is accompanied by sustained DNA damage and hypersensitivity to genotoxic stress. Collectively, our study uncovers a chromatin-bound factor that serves an accessory role in coupling damage signaling with chromatin changes in response to DNA damage. © 2010 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/molcelen_HK
dc.relation.ispartofMolecular Cellen_HK
dc.subjectDNAen_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshChromatin - genetics - metabolism-
dc.subject.meshChromosomal Proteins, Non-Histone - chemistry - genetics - metabolism-
dc.subject.meshDNA Damage-
dc.titleRegulation of Chromatin Architecture by the PWWP Domain-Containing DNA Damage-Responsive Factor EXPAND1/MUM1en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1097-2765&volume=37&issue=6&spage=854&epage=864&date=2010&atitle=Regulation+of+chromatin+architecture+by+the+PWWP+domain-containing+DNA+damage-responsive+factor+EXPAND1/MUM1en_HK
dc.identifier.emailHuen, MSY:huen.michael@hku.hken_HK
dc.identifier.emailChing, YP:ypching@hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityHuen, MSY=rp01336en_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.molcel.2009.12.040en_HK
dc.identifier.pmid20347427en_HK
dc.identifier.scopuseid_2-s2.0-77949708459en_HK
dc.identifier.hkuros169512-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77949708459&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue6en_HK
dc.identifier.spage854en_HK
dc.identifier.epage864en_HK
dc.identifier.eissn1097-4164-
dc.identifier.isiWOS:000276135100012-
dc.publisher.placeUnited Statesen_HK
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dc.identifier.scopusauthoridHuen, MSY=23004751500en_HK
dc.identifier.scopusauthoridHuang, J=24467982900en_HK
dc.identifier.scopusauthoridLeung, JWC=35750178400en_HK
dc.identifier.scopusauthoridSy, SMH=6602984466en_HK
dc.identifier.scopusauthoridLeung, KM=7401860685en_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridChen, J=7501899830en_HK
dc.identifier.citeulike6959115-

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