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Article: Sex hormone-induced mammary carcinogenesis in female Noble rats: Detection of differentially expressed genes

TitleSex hormone-induced mammary carcinogenesis in female Noble rats: Detection of differentially expressed genes
Authors
Issue Date2003
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-6806
Citation
Breast Cancer Research And Treatment, 2003, v. 77 n. 1, p. 49-63 How to Cite?
AbstractBreast cancer is the most common cancer and the second most frequent cause of cancer death in women. Epidemiological data has recognized that an increased cumulative exposure to estrogen is the common tie linking most of the established risk factors for breast cancer. Sex hormone-induced mammary gland carcinogenesis of the Noble rat (using testosterone and 17β-estradiol) resembles that of the human counterpart in its growth pattern as well as the histopathology of the tumors induced. This model may provide a paradigm for examination of genetic alterations and changes in gene expression between different histological groups and to make inferences about the role of known and putative oncogenes and tumor suppressor genes. We studied the gene expression profile during sex hormone-induced mammary carcinogenesis using a cDNA array technique; the results were further confirmed by RT-PCR, western blotting and immunohistochemical analyses. From the 10 differentially expressed genes identified, we have studied four highly overexpressed genes, two cell cycle/growth control regulators, the cyclins D1 and D2, a growth factor, IGF-2 and a cytokine TNF-α. Cyclins D1 and D2 were highly expressed in the nuclei of carcinoma cells but at low levels in the nuclei of the hyperplastic and normal mammary tissue. IGF-2 was found to expressed in the cytoplasm of the carcinoma cells but not in the stromal cells. Western blot showed expression of big IGF-2 consistent with the tumor derived truncated forms of pro-IGF-2. The matured circulating IGF-2 at 7.5 kDa identified in the serum was not expressed in any of the breast tissue samples. TNF-α expression was found not only in the macrophages but also in the mammary carcinoma cells. The result of the present study provides some information on the molecular basis of this sex hormone-induced mammary carcinogenesis and the role of these proteins in tumor progression.
Persistent Identifierhttp://hdl.handle.net/10722/67494
ISSN
2015 Impact Factor: 4.085
2015 SCImago Journal Rankings: 2.424
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, Gen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorWong, YCen_HK
dc.date.accessioned2010-09-06T05:55:38Z-
dc.date.available2010-09-06T05:55:38Z-
dc.date.issued2003en_HK
dc.identifier.citationBreast Cancer Research And Treatment, 2003, v. 77 n. 1, p. 49-63en_HK
dc.identifier.issn0167-6806en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67494-
dc.description.abstractBreast cancer is the most common cancer and the second most frequent cause of cancer death in women. Epidemiological data has recognized that an increased cumulative exposure to estrogen is the common tie linking most of the established risk factors for breast cancer. Sex hormone-induced mammary gland carcinogenesis of the Noble rat (using testosterone and 17β-estradiol) resembles that of the human counterpart in its growth pattern as well as the histopathology of the tumors induced. This model may provide a paradigm for examination of genetic alterations and changes in gene expression between different histological groups and to make inferences about the role of known and putative oncogenes and tumor suppressor genes. We studied the gene expression profile during sex hormone-induced mammary carcinogenesis using a cDNA array technique; the results were further confirmed by RT-PCR, western blotting and immunohistochemical analyses. From the 10 differentially expressed genes identified, we have studied four highly overexpressed genes, two cell cycle/growth control regulators, the cyclins D1 and D2, a growth factor, IGF-2 and a cytokine TNF-α. Cyclins D1 and D2 were highly expressed in the nuclei of carcinoma cells but at low levels in the nuclei of the hyperplastic and normal mammary tissue. IGF-2 was found to expressed in the cytoplasm of the carcinoma cells but not in the stromal cells. Western blot showed expression of big IGF-2 consistent with the tumor derived truncated forms of pro-IGF-2. The matured circulating IGF-2 at 7.5 kDa identified in the serum was not expressed in any of the breast tissue samples. TNF-α expression was found not only in the macrophages but also in the mammary carcinoma cells. The result of the present study provides some information on the molecular basis of this sex hormone-induced mammary carcinogenesis and the role of these proteins in tumor progression.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-6806en_HK
dc.relation.ispartofBreast Cancer Research and Treatmenten_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCyclin Den_HK
dc.subject.meshCyclins - geneticsen_HK
dc.subject.meshEstradiol - pharmacologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshInsulin-Like Growth Factor II - geneticsen_HK
dc.subject.meshMammary Neoplasms, Experimental - chemically induced - geneticsen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Inbred Strainsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTestosterone - pharmacologyen_HK
dc.subject.meshTumor Necrosis Factor-alpha - geneticsen_HK
dc.titleSex hormone-induced mammary carcinogenesis in female Noble rats: Detection of differentially expressed genesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-6806&volume=77&spage=49&epage=63&date=2003&atitle=Sex+hormone-induced+mammary+carcinogenesis+in+female+Noble+rats:+detection+of+differentially+expressed+genesen_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1021123914339en_HK
dc.identifier.pmid12602904-
dc.identifier.scopuseid_2-s2.0-0037263316en_HK
dc.identifier.hkuros75845en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037263316&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume77en_HK
dc.identifier.issue1en_HK
dc.identifier.spage49en_HK
dc.identifier.epage63en_HK
dc.identifier.isiWOS:000179341300006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLeung, G=26650696900en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK

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