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Article: CNTF and BDNF have similar effects on retinal ganglion cell survival but differential effects on nitric oxide synthase expression soon after optic nerve injury

TitleCNTF and BDNF have similar effects on retinal ganglion cell survival but differential effects on nitric oxide synthase expression soon after optic nerve injury
Authors
Issue Date2005
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2005, v. 46 n. 4, p. 1497-1503 How to Cite?
AbstractPURPOSE. To investigate the effect of ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) on retinal ganglion cell (RGC) survival and nitric oxide synthase (NOS) expression in the retina during the early phase of optic nerve (ON) injury, and to examine whether intraperitoneal application of the NOS scavenger nitro-L-arginine (L-NA) could protect the injured RGCs. METHODS. RGCs were retrogradely labeled with granular blue 3 days before the ON was intraorbitally transected. RGC survival was examined 1 week after ON transection and intraocular injection of CNTF and/or BDNF, or 1 to 2 weeks after daily intraperitoneal injection of the NOS inhibitor L-NA. NOS expression was examined by NADPH-diaphorase histochemistry and neuronal NOS (nNOS) immunohistochemistry, and nNOS-positive cells were identified by various staining approaches. RESULTS. Both CNTF and BDNF significantly increased RGC survival 1 week after ON injury. In the ganglion cell layer (GCL), CNTF did not increase the number of NADPH-diaphorase positive (+) cells but appeared to reduce the intensity of NADPH-diaphorase staining, whereas BDNF increased the number of NADPH-diaphorase+ cells and also appeared to enhance the intensity of NADPH-diaphorase staining. In the GCL, amacrine cells but not RGCs were nNOS+. Some macrophages were also nNOS+. In contrast, no amacrine cells were nNOS+ in the inner nuclear layer. Daily intraperitoneal injection of L-NA at appropriate concentrations promoted RGC survival for 1 or 2 weeks after ON injury. CONCLUSIONS. Both CNTF and BDNF protected RGCs after ON injury. CNTF and BDNF acted differently on NOS expression in the GCL. Intraperitoneal injections of L-NA at appropriate dosages enhance RGC survival. Copyright © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/67489
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, CWen_HK
dc.contributor.authorLu, Qen_HK
dc.contributor.authorYou, SWen_HK
dc.contributor.authorZhi, Yen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorWu, Wen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorCui, Qen_HK
dc.date.accessioned2010-09-06T05:55:35Z-
dc.date.available2010-09-06T05:55:35Z-
dc.date.issued2005en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2005, v. 46 n. 4, p. 1497-1503en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67489-
dc.description.abstractPURPOSE. To investigate the effect of ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) on retinal ganglion cell (RGC) survival and nitric oxide synthase (NOS) expression in the retina during the early phase of optic nerve (ON) injury, and to examine whether intraperitoneal application of the NOS scavenger nitro-L-arginine (L-NA) could protect the injured RGCs. METHODS. RGCs were retrogradely labeled with granular blue 3 days before the ON was intraorbitally transected. RGC survival was examined 1 week after ON transection and intraocular injection of CNTF and/or BDNF, or 1 to 2 weeks after daily intraperitoneal injection of the NOS inhibitor L-NA. NOS expression was examined by NADPH-diaphorase histochemistry and neuronal NOS (nNOS) immunohistochemistry, and nNOS-positive cells were identified by various staining approaches. RESULTS. Both CNTF and BDNF significantly increased RGC survival 1 week after ON injury. In the ganglion cell layer (GCL), CNTF did not increase the number of NADPH-diaphorase positive (+) cells but appeared to reduce the intensity of NADPH-diaphorase staining, whereas BDNF increased the number of NADPH-diaphorase+ cells and also appeared to enhance the intensity of NADPH-diaphorase staining. In the GCL, amacrine cells but not RGCs were nNOS+. Some macrophages were also nNOS+. In contrast, no amacrine cells were nNOS+ in the inner nuclear layer. Daily intraperitoneal injection of L-NA at appropriate concentrations promoted RGC survival for 1 or 2 weeks after ON injury. CONCLUSIONS. Both CNTF and BDNF protected RGCs after ON injury. CNTF and BDNF acted differently on NOS expression in the GCL. Intraperitoneal injections of L-NA at appropriate dosages enhance RGC survival. Copyright © Association for Research in Vision and Ophthalmology.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.titleCNTF and BDNF have similar effects on retinal ganglion cell survival but differential effects on nitric oxide synthase expression soon after optic nerve injuryen_HK
dc.typeArticleen_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.identifier.authorityWu, W=rp00419en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1167/iovs.04-0664en_HK
dc.identifier.pmid15790921-
dc.identifier.scopuseid_2-s2.0-18244380339en_HK
dc.identifier.hkuros97774en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18244380339&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1497en_HK
dc.identifier.epage1503en_HK
dc.identifier.isiWOS:000227908900055-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, CW=8226423900en_HK
dc.identifier.scopusauthoridLu, Q=54902894100en_HK
dc.identifier.scopusauthoridYou, SW=8226423300en_HK
dc.identifier.scopusauthoridZhi, Y=8618487800en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK
dc.identifier.scopusauthoridWu, W=7407081122en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridCui, Q=7103080164en_HK

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