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Article: BAD and Bcl-2 regulation are early events linking neuronal endoplasmic reticulum stress to mitochondria-mediated apoptosis

TitleBAD and Bcl-2 regulation are early events linking neuronal endoplasmic reticulum stress to mitochondria-mediated apoptosis
Authors
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/molbrainres
Citation
Molecular Brain Research, 2002, v. 109 n. 1-2, p. 233-238 How to Cite?
AbstractThe mechanism of endoplasmic reticulum (ER)-mediated apoptosis in neurons was examined. Using primary cortical neurons, we show that nordihydroguaiaretic acid (NDGA) and brefeldin A (BFA), two ER stressors, induce early ER stress as shown by Western blotting of the eukaryotic initiation factor-2α (eIF2α), an ER stress marker. This event was associated with an enhancement of neuronal apoptosis as demonstrated by the time-dependent increase in caspase-3 activity and by nuclear fragmentation. The study of the apoptotic signaling showed the translocation of cytochrome c from the mitochondrial matrix to the cytosol. Further evaluation of the apoptotic process revealed that NDGA and BFA induced a rapid dephosphorylation of BAD and decrease expression of Bcl-2. Altogether, our results indicate that neuronal ER stress is associated with an apoptotic cascade involving the mitochondria. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67484
ISSN
2007 Impact Factor: 1.997
2008 SCImago Journal Rankings: 1.457
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorElyaman, Wen_HK
dc.contributor.authorTerro, Fen_HK
dc.contributor.authorSuen, KCen_HK
dc.contributor.authorYardin, Cen_HK
dc.contributor.authorChang, RCCen_HK
dc.contributor.authorHugon, Jen_HK
dc.date.accessioned2010-09-06T05:55:32Z-
dc.date.available2010-09-06T05:55:32Z-
dc.date.issued2002en_HK
dc.identifier.citationMolecular Brain Research, 2002, v. 109 n. 1-2, p. 233-238en_HK
dc.identifier.issn0169-328Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67484-
dc.description.abstractThe mechanism of endoplasmic reticulum (ER)-mediated apoptosis in neurons was examined. Using primary cortical neurons, we show that nordihydroguaiaretic acid (NDGA) and brefeldin A (BFA), two ER stressors, induce early ER stress as shown by Western blotting of the eukaryotic initiation factor-2α (eIF2α), an ER stress marker. This event was associated with an enhancement of neuronal apoptosis as demonstrated by the time-dependent increase in caspase-3 activity and by nuclear fragmentation. The study of the apoptotic signaling showed the translocation of cytochrome c from the mitochondrial matrix to the cytosol. Further evaluation of the apoptotic process revealed that NDGA and BFA induced a rapid dephosphorylation of BAD and decrease expression of Bcl-2. Altogether, our results indicate that neuronal ER stress is associated with an apoptotic cascade involving the mitochondria. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/molbrainresen_HK
dc.relation.ispartofMolecular Brain Researchen_HK
dc.rightsMolecular Brain Research. Copyright © Elsevier BV.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntioxidants - pharmacologyen_HK
dc.subject.meshApoptosis - physiologyen_HK
dc.subject.meshBrefeldin A - pharmacologyen_HK
dc.subject.meshCarrier Proteins - metabolismen_HK
dc.subject.meshCaspase 3en_HK
dc.subject.meshCaspases - metabolismen_HK
dc.subject.meshCell Fractionationen_HK
dc.subject.meshCell Nucleus - metabolismen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCerebral Cortex - cytology - metabolismen_HK
dc.subject.meshEndoplasmic Reticulum - drug effects - metabolismen_HK
dc.subject.meshEnzyme Activationen_HK
dc.subject.meshEukaryotic Initiation Factor-2 - metabolismen_HK
dc.subject.meshMitochondria - metabolismen_HK
dc.subject.meshNeurons - cytology - drug effects - metabolismen_HK
dc.subject.meshNordihydroguaiaretic Acid - pharmacologyen_HK
dc.subject.meshProtein Synthesis Inhibitors - pharmacologyen_HK
dc.subject.meshProto-Oncogene Proteins c-bcl-2 - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshbcl-Associated Death Proteinen_HK
dc.titleBAD and Bcl-2 regulation are early events linking neuronal endoplasmic reticulum stress to mitochondria-mediated apoptosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0169-328X&volume=109&spage=233&epage=238&date=2002&atitle=BAD+and+Bcl-2+regulation+are+early+events+linking+neuronal+endoplasmic+reticulum+stress+to+mitochondria-mediated+apoptosisen_HK
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0169-328X(02)00582-Xen_HK
dc.identifier.pmid12531534-
dc.identifier.scopuseid_2-s2.0-12244288323en_HK
dc.identifier.hkuros75834en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-12244288323&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume109en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage233en_HK
dc.identifier.epage238en_HK
dc.identifier.isiWOS:000180752600027-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridElyaman, W=6603236614en_HK
dc.identifier.scopusauthoridTerro, F=6602670596en_HK
dc.identifier.scopusauthoridSuen, KC=7004577222en_HK
dc.identifier.scopusauthoridYardin, C=6701328640en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK
dc.identifier.scopusauthoridHugon, J=7103202992en_HK

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