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Article: Characterization of spinal motoneuron degeneration following different types of peripheral nerve injury in neonatal and adult mice

TitleCharacterization of spinal motoneuron degeneration following different types of peripheral nerve injury in neonatal and adult mice
Authors
Issue Date1998
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 1998, v. 396 n. 2, p. 158-168 How to Cite?
AbstractExperimental lesions have been used widely to induce motoneuron (MN) degeneration as a model to test the ability of different trophic molecules to prevent lesion-induced alterations. However, the morphological mechanisms of spinal MN death following different types of lesions is not clear at the present time. In this study, we have characterized the morphological characteristics of MN cell death by examining DNA fragmentation and the ultrastructural and light microscopic morphological features of MNs following different types of spinal nerve injury (i.e., axotomy and avulsion) in the developing and adult mouse. In neonatal mice, axotomy induced cell death as well as the atrophy of MNs that survived the injury. DNA fragmentation could be detected by using the terminal deoxynucleotidyl transferase (TUNEL) method during the cell death process following neonatal axotomy, whereas TUNEL labeling was not observed following either neonatal or adult avulsion. However, with the exception of TUNEL labeling, the morphological characteristics of MN death following neonatal axotomy and avulsion were similar, and both resembled most closely the form of programmed cell death termed cytoplasmic or type 3B, which exhibits similarities as well as differences with currently accepted definitions of apoptosis. By contrast, adult avulsion resulted in a type of degeneration that resembled necrosis more closely. However, even there, the morphology was mixed, showing characteristics of both apoptosis and necrosis. These results indicate that the mode of MN degeneration is complex and is related to developmental age and type of lesion.
Persistent Identifierhttp://hdl.handle.net/10722/67467
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Len_HK
dc.contributor.authorHouenou, LJen_HK
dc.contributor.authorWu, Wen_HK
dc.contributor.authorLei, Men_HK
dc.contributor.authorPrevette, DMen_HK
dc.contributor.authorOppenheim, RWen_HK
dc.date.accessioned2010-09-06T05:55:24Z-
dc.date.available2010-09-06T05:55:24Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Comparative Neurology, 1998, v. 396 n. 2, p. 158-168en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67467-
dc.description.abstractExperimental lesions have been used widely to induce motoneuron (MN) degeneration as a model to test the ability of different trophic molecules to prevent lesion-induced alterations. However, the morphological mechanisms of spinal MN death following different types of lesions is not clear at the present time. In this study, we have characterized the morphological characteristics of MN cell death by examining DNA fragmentation and the ultrastructural and light microscopic morphological features of MNs following different types of spinal nerve injury (i.e., axotomy and avulsion) in the developing and adult mouse. In neonatal mice, axotomy induced cell death as well as the atrophy of MNs that survived the injury. DNA fragmentation could be detected by using the terminal deoxynucleotidyl transferase (TUNEL) method during the cell death process following neonatal axotomy, whereas TUNEL labeling was not observed following either neonatal or adult avulsion. However, with the exception of TUNEL labeling, the morphological characteristics of MN death following neonatal axotomy and avulsion were similar, and both resembled most closely the form of programmed cell death termed cytoplasmic or type 3B, which exhibits similarities as well as differences with currently accepted definitions of apoptosis. By contrast, adult avulsion resulted in a type of degeneration that resembled necrosis more closely. However, even there, the morphology was mixed, showing characteristics of both apoptosis and necrosis. These results indicate that the mode of MN degeneration is complex and is related to developmental age and type of lesion.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnimals, Newbornen_HK
dc.subject.meshApoptosis - geneticsen_HK
dc.subject.meshAxotomyen_HK
dc.subject.meshDNA Fragmentationen_HK
dc.subject.meshDNA Nucleotidylexotransferaseen_HK
dc.subject.meshGenetic Techniquesen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshMicroscopy, Electronen_HK
dc.subject.meshMotor Neurons - pathologyen_HK
dc.subject.meshNecrosisen_HK
dc.subject.meshNerve Degeneration - pathologyen_HK
dc.subject.meshPeripheral Nerve Injuriesen_HK
dc.subject.meshSpinal Nerves - growth & development - pathologyen_HK
dc.titleCharacterization of spinal motoneuron degeneration following different types of peripheral nerve injury in neonatal and adult miceen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_HK
dc.identifier.authorityWu, W=rp00419en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1096-9861(19980629)396:2<158::AID-CNE2>3.0.CO;2-#en_HK
dc.identifier.pmid9634139-
dc.identifier.scopuseid_2-s2.0-0032578036en_HK
dc.identifier.hkuros36834en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032578036&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume396en_HK
dc.identifier.issue2en_HK
dc.identifier.spage158en_HK
dc.identifier.epage168en_HK
dc.identifier.isiWOS:000073912000002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, L=9038682000en_HK
dc.identifier.scopusauthoridHouenou, LJ=7003871997en_HK
dc.identifier.scopusauthoridWu, W=7407081122en_HK
dc.identifier.scopusauthoridLei, M=54887929900en_HK
dc.identifier.scopusauthoridPrevette, DM=7003628635en_HK
dc.identifier.scopusauthoridOppenheim, RW=7102628195en_HK

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