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Article: Establishment and characterization of an immortalized human oviductal cell line

TitleEstablishment and characterization of an immortalized human oviductal cell line
Authors
KeywordsCoculture
Cytokeratin
HPV 16 E6/E7
Human oviduct-specific glycoprotein
Immortalization
Telomerase
Issue Date2001
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692
Citation
Molecular Reproduction And Development, 2001, v. 59 n. 4, p. 400-409 How to Cite?
AbstractHuman oviductal cells stimulate embryo development in vitro partly by the production of embryotrophic glycoproteins. The identity of these glycoproteins is not yet known mainly because oviductal samples are limited and that the cultured parental oviductal cells cannot produce sufficient amount of embryotrophic factors for characterization. In this study, human oviductal epithelial cells (OE) were immortalized by HPV 16 E6/E7 open reading frame (ORF) by retroviral expression. The characteristics of this immortalized cell line (OE-E6/ET) were compared to the parental OE. HPV 16 E6/E7 DNA was found only in OE-E6/E7 but not in OE. Human oviduct-specific glycoprotein, estrogen receptors, and cytokeratin were found in both cell types. Both OE and OE-E6/ E7 possessed telomerase activities but the former had much lower activity. OE-E6/E7 also produced glycoproteins with chromatographic behavior similar to the embryotrophic glycoproteins derived from OE. These results showed that OE-E6/E7 retained a number of characteristics of OE. The development of preimplantation mouse embryo was significantly better after coculture with OE-E6/E7 when compared to medium alone culture in term of blastulation rates (52% vs. 32%) and blastocyst diameter (113.0± 2.07 μm vs. 83.9±5.23 μm). This immortalized cell line can be used as a continuous and stable in vitro system for the study of the oviductal embryotrophic activity. © 200l Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/67465
ISSN
2015 Impact Factor: 2.141
2015 SCImago Journal Rankings: 1.041
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, YLen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorXu, JSen_HK
dc.contributor.authorWang, YLen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T05:55:22Z-
dc.date.available2010-09-06T05:55:22Z-
dc.date.issued2001en_HK
dc.identifier.citationMolecular Reproduction And Development, 2001, v. 59 n. 4, p. 400-409en_HK
dc.identifier.issn1040-452Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67465-
dc.description.abstractHuman oviductal cells stimulate embryo development in vitro partly by the production of embryotrophic glycoproteins. The identity of these glycoproteins is not yet known mainly because oviductal samples are limited and that the cultured parental oviductal cells cannot produce sufficient amount of embryotrophic factors for characterization. In this study, human oviductal epithelial cells (OE) were immortalized by HPV 16 E6/E7 open reading frame (ORF) by retroviral expression. The characteristics of this immortalized cell line (OE-E6/ET) were compared to the parental OE. HPV 16 E6/E7 DNA was found only in OE-E6/E7 but not in OE. Human oviduct-specific glycoprotein, estrogen receptors, and cytokeratin were found in both cell types. Both OE and OE-E6/ E7 possessed telomerase activities but the former had much lower activity. OE-E6/E7 also produced glycoproteins with chromatographic behavior similar to the embryotrophic glycoproteins derived from OE. These results showed that OE-E6/E7 retained a number of characteristics of OE. The development of preimplantation mouse embryo was significantly better after coculture with OE-E6/E7 when compared to medium alone culture in term of blastulation rates (52% vs. 32%) and blastocyst diameter (113.0± 2.07 μm vs. 83.9±5.23 μm). This immortalized cell line can be used as a continuous and stable in vitro system for the study of the oviductal embryotrophic activity. © 200l Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692en_HK
dc.relation.ispartofMolecular Reproduction and Developmenten_HK
dc.rightsMolecular Reproduction and Development . Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectCoculture-
dc.subjectCytokeratin-
dc.subjectHPV 16 E6/E7-
dc.subjectHuman oviduct-specific glycoprotein-
dc.subjectImmortalization-
dc.subjectTelomerase-
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCell Transformation, Viralen_HK
dc.subject.meshCoculture Techniquesen_HK
dc.subject.meshCulture Media, Conditioned - chemistryen_HK
dc.subject.meshEmbryo, Mammalian - physiologyen_HK
dc.subject.meshEpithelial Cells - physiologyen_HK
dc.subject.meshFallopian Tubes - cytologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGlycoproteins - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoblottingen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshKeratins - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshPapillomaviridae - geneticsen_HK
dc.subject.meshPhenotypeen_HK
dc.subject.meshReceptors, Estrogen - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTelomerase - metabolismen_HK
dc.titleEstablishment and characterization of an immortalized human oviductal cell lineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1040-452X&volume=59&spage=400&epage=409&date=2001&atitle=Establishment+and+characterization+of+an+immortalized+human+oviductal+cell+line.en_HK
dc.identifier.emailLee, YL:h9316321@hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mrd.1046en_HK
dc.identifier.pmid11468776-
dc.identifier.scopuseid_2-s2.0-0034957211en_HK
dc.identifier.hkuros112505en_HK
dc.identifier.hkuros65582-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034957211&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue4en_HK
dc.identifier.spage400en_HK
dc.identifier.epage409en_HK
dc.identifier.isiWOS:000169590000007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridXu, JS=7408556691en_HK
dc.identifier.scopusauthoridWang, YL=7601492022en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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