File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Expression of a kallikrein-like protein in prostatic intraepithelial neoplasia in ventral prostate of the noble rat

TitleExpression of a kallikrein-like protein in prostatic intraepithelial neoplasia in ventral prostate of the noble rat
Authors
KeywordsKallikrein-like protein
Prostatic intraepithelial neoplasia
Secretion
Ventral prostate
Issue Date2000
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 2000, v. 42 n. 1, p. 8-17 How to Cite?
AbstractBACKGROUND. In an effort to identify biomarker(s) for prostatic cancer (PCa), we analyzed the changes of secretory proteins in the ventral prostate (VP) of Noble rats at early stages of carcinogenesis. METHODS. Ventral prostates were removed from both control (n = 36) and experimental (n = 88) rats implanted with a known ratio of testosterone (T) and 17β-estradiol (E2). Tissue sections were stained by hematoxylin and eosin (H and E) for pathological screening, and secretions were collected for SDS-PAGE analysis followed by N-terminal microsequencing, antiserum production, Western blot, and immunohistochemical study. RESULTS. Pathologically, low-grade prostatic intraepithelial neoplasia (LGPIN) and high-grade PIN (HGPIN) were observed in ducts or alveoli after 3 and 5 months of T + E2 treatment, respectively. The results of SDS-PAGE showed an elevated expression of 18-kDa protein (p18) in secretions of VP with HGPIN or cancerous lesions. Analysis of p18 by N- terminal sequencing showed a high score of homology to rat glandular kallikrein. To characterize the expression pattern of the protein in tissue samples, an antiserum was raised against the N-terminus of the p18. The monospecificity of the antiserum against p18 was confirmed by Western blot analysis. Immunohistochemical study showed that in ducts or alveoli of normal and LGPIN samples, a mild positive staining for p18 was observed in secretions. However, the reactivity was intense not only in luminal secretions but also in some luminal secretory cells in HGPIN and cancer cells as well. CONCLUSIONS. The high expression of p18 in connection with neoplastic transformation of cells strongly suggests that the potential application of this protein as a marker for early detection of PCa should be further investigated.
Persistent Identifierhttp://hdl.handle.net/10722/67440
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXie, Wen_HK
dc.contributor.authorWong, YCen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorWong, NSen_HK
dc.date.accessioned2010-09-06T05:55:09Z-
dc.date.available2010-09-06T05:55:09Z-
dc.date.issued2000en_HK
dc.identifier.citationProstate, 2000, v. 42 n. 1, p. 8-17en_HK
dc.identifier.issn0270-4137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67440-
dc.description.abstractBACKGROUND. In an effort to identify biomarker(s) for prostatic cancer (PCa), we analyzed the changes of secretory proteins in the ventral prostate (VP) of Noble rats at early stages of carcinogenesis. METHODS. Ventral prostates were removed from both control (n = 36) and experimental (n = 88) rats implanted with a known ratio of testosterone (T) and 17β-estradiol (E2). Tissue sections were stained by hematoxylin and eosin (H and E) for pathological screening, and secretions were collected for SDS-PAGE analysis followed by N-terminal microsequencing, antiserum production, Western blot, and immunohistochemical study. RESULTS. Pathologically, low-grade prostatic intraepithelial neoplasia (LGPIN) and high-grade PIN (HGPIN) were observed in ducts or alveoli after 3 and 5 months of T + E2 treatment, respectively. The results of SDS-PAGE showed an elevated expression of 18-kDa protein (p18) in secretions of VP with HGPIN or cancerous lesions. Analysis of p18 by N- terminal sequencing showed a high score of homology to rat glandular kallikrein. To characterize the expression pattern of the protein in tissue samples, an antiserum was raised against the N-terminus of the p18. The monospecificity of the antiserum against p18 was confirmed by Western blot analysis. Immunohistochemical study showed that in ducts or alveoli of normal and LGPIN samples, a mild positive staining for p18 was observed in secretions. However, the reactivity was intense not only in luminal secretions but also in some luminal secretory cells in HGPIN and cancer cells as well. CONCLUSIONS. The high expression of p18 in connection with neoplastic transformation of cells strongly suggests that the potential application of this protein as a marker for early detection of PCa should be further investigated.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_HK
dc.relation.ispartofProstateen_HK
dc.rightsThe Prostate. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectKallikrein-like proteinen_HK
dc.subjectProstatic intraepithelial neoplasiaen_HK
dc.subjectSecretionen_HK
dc.subjectVentral prostateen_HK
dc.subject.meshAdenocarcinoma - metabolism - pathologyen_HK
dc.subject.meshAmino Acid Sequence - geneticsen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshProstate - metabolismen_HK
dc.subject.meshProstatic Intraepithelial Neoplasia - metabolism - pathologyen_HK
dc.subject.meshProstatic Neoplasms - metabolism - pathologyen_HK
dc.subject.meshProteins - genetics - metabolism - secretionen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Inbred Strainsen_HK
dc.titleExpression of a kallikrein-like protein in prostatic intraepithelial neoplasia in ventral prostate of the noble raten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-4137&volume=42&spage=8&epage=17&date=2000&atitle=Expression+of+a+kallikrein-like+protein+in+prostatic+intraepithelial+neoplasia+in+ventral+prostate+of+the+noble+raten_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailWong, NS:nswong@hkucc.hku.hken_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityWong, NS=rp00340en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1097-0045(20000101)42:1<8::AID-PROS2>3.0.CO;2-Oen_HK
dc.identifier.pmid10579794-
dc.identifier.scopuseid_2-s2.0-0033989356en_HK
dc.identifier.hkuros47805en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033989356&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue1en_HK
dc.identifier.spage8en_HK
dc.identifier.epage17en_HK
dc.identifier.isiWOS:000084207100002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXie, W=21647230200en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridWong, NS=7202836641en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats