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Article: Male Genital Tract Antioxidant Enzymes: Their Source, Function in the Female, and Ability to Preserve Sperm DNA Integrity in the Golden Hamster

TitleMale Genital Tract Antioxidant Enzymes: Their Source, Function in the Female, and Ability to Preserve Sperm DNA Integrity in the Golden Hamster
Authors
KeywordsCatalase
Glutathione peroxidase
Oxidative stress
Sperm DNA damage
Superoxide dismutase
Syrian hamster
Issue Date2003
PublisherAmerican Society of Andrology. The Journal's web site is located at http://www.andrologyjournal.org
Citation
Journal Of Andrology, 2003, v. 24 n. 5, p. 704-711 How to Cite?
AbstractRecently, we reported that male accessory sex gland (ASG) secretions protect sperm genomic integrity by demonstrating that DNA damage was more extensive in sperm not exposed to the secretions. The present study was conducted to find out if ASGs secrete the main antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx or GSH-Px), and catalase (CAT) and if the most abundant one, SOD, can protect those sperm that were not exposed to ASG secretions against NADPH-induced oxidative stress. Four experimental groups of male golden hamsters were used: intact animals with proven fertility, animals with all major ASGs removed (TX), animals that were bilaterally vasectomized, and sham-operated controls. SOD, CAT, and GPx activities were measured in secretions from all 5 ASGs and sperm-free uterine flushing from virgin females and those mated with the experimental males. The alkaline comet assay was used to analyze DNA integrity of the TX group sperm after incubation in a medium containing 50 U/mL of SOD along with 0 to 20 mmol/L NADPH. The main antioxidant enzyme in ASGs was SOD from coagulating glands (P < .05) and GPx together with CAT from ampullary glands (P < .05). Uterine flushing of ejaculates that contained ASG secretions had more SOD and CAT activities than those with epididymal secretions alone (P < .05 and P < .001, respectively), whereas activity of GPx was the same (P > .05). Addition of SOD in vitro dose dependently decreased the incidence of single-strand DNA damage in sperm not exposed to ASG secretions incubated in the presence of 0 to 20 mmol/L NADPH (P < .001). These results indicated that, in terms of abundance, SOD was the main antioxidant enzyme secreted by male ASGs, whereas CAT was the second one. The GPx activity came from both epididymis and ASGs. We conclude that ASG secretions play a significant role in protecting sperm against oxidative stress.
Persistent Identifierhttp://hdl.handle.net/10722/67429
ISSN
2014 Impact Factor: 2.473
2015 SCImago Journal Rankings: 0.867
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Hen_HK
dc.contributor.authorChow, PHen_HK
dc.contributor.authorCheng, SKen_HK
dc.contributor.authorCheung, ALMen_HK
dc.contributor.authorCheng, LYLen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-09-06T05:55:04Z-
dc.date.available2010-09-06T05:55:04Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Andrology, 2003, v. 24 n. 5, p. 704-711en_HK
dc.identifier.issn0196-3635en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67429-
dc.description.abstractRecently, we reported that male accessory sex gland (ASG) secretions protect sperm genomic integrity by demonstrating that DNA damage was more extensive in sperm not exposed to the secretions. The present study was conducted to find out if ASGs secrete the main antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx or GSH-Px), and catalase (CAT) and if the most abundant one, SOD, can protect those sperm that were not exposed to ASG secretions against NADPH-induced oxidative stress. Four experimental groups of male golden hamsters were used: intact animals with proven fertility, animals with all major ASGs removed (TX), animals that were bilaterally vasectomized, and sham-operated controls. SOD, CAT, and GPx activities were measured in secretions from all 5 ASGs and sperm-free uterine flushing from virgin females and those mated with the experimental males. The alkaline comet assay was used to analyze DNA integrity of the TX group sperm after incubation in a medium containing 50 U/mL of SOD along with 0 to 20 mmol/L NADPH. The main antioxidant enzyme in ASGs was SOD from coagulating glands (P < .05) and GPx together with CAT from ampullary glands (P < .05). Uterine flushing of ejaculates that contained ASG secretions had more SOD and CAT activities than those with epididymal secretions alone (P < .05 and P < .001, respectively), whereas activity of GPx was the same (P > .05). Addition of SOD in vitro dose dependently decreased the incidence of single-strand DNA damage in sperm not exposed to ASG secretions incubated in the presence of 0 to 20 mmol/L NADPH (P < .001). These results indicated that, in terms of abundance, SOD was the main antioxidant enzyme secreted by male ASGs, whereas CAT was the second one. The GPx activity came from both epididymis and ASGs. We conclude that ASG secretions play a significant role in protecting sperm against oxidative stress.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Andrology. The Journal's web site is located at http://www.andrologyjournal.orgen_HK
dc.relation.ispartofJournal of Andrologyen_HK
dc.subjectCatalaseen_HK
dc.subjectGlutathione peroxidaseen_HK
dc.subjectOxidative stressen_HK
dc.subjectSperm DNA damageen_HK
dc.subjectSuperoxide dismutaseen_HK
dc.subjectSyrian hamsteren_HK
dc.subject.meshAlkaliesen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntioxidants - metabolismen_HK
dc.subject.meshCatalase - metabolismen_HK
dc.subject.meshCopulationen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshDNA Damage - physiologyen_HK
dc.subject.meshDNA, Single-Stranded - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGenitalia, Male - enzymologyen_HK
dc.subject.meshGlutathione Peroxidase - metabolismen_HK
dc.subject.meshHydrogen-Ion Concentrationen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMesocricetusen_HK
dc.subject.meshOxidative Stress - physiologyen_HK
dc.subject.meshSemen - enzymologyen_HK
dc.subject.meshSpermatozoa - physiologyen_HK
dc.subject.meshSuperoxide Dismutase - metabolismen_HK
dc.subject.meshTherapeutic Irrigationen_HK
dc.subject.meshUterusen_HK
dc.titleMale Genital Tract Antioxidant Enzymes: Their Source, Function in the Female, and Ability to Preserve Sperm DNA Integrity in the Golden Hamsteren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0196-3635&volume=24 No 5&spage=704&epage=711&date=2003&atitle=Male+genital+tract+antioxidant+enzymes:+their+source,+function+in+the+female,+and+ability+to+preserve+sperm+DNA+integrity+in+the+golden+hamsteren_HK
dc.identifier.emailCheung, ALM:lmcheung@hkucc.hku.hken_HK
dc.identifier.emailCheng, LYL:lcheng@hkucc.hku.hken_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ALM=rp00332en_HK
dc.identifier.authorityCheng, LYL=rp00317en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid12954661-
dc.identifier.scopuseid_2-s2.0-0642335997en_HK
dc.identifier.hkuros88186en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0642335997&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue5en_HK
dc.identifier.spage704en_HK
dc.identifier.epage711en_HK
dc.identifier.isiWOS:000185347400012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, H=7501618169en_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK
dc.identifier.scopusauthoridCheng, SK=7404685463en_HK
dc.identifier.scopusauthoridCheung, ALM=7401806497en_HK
dc.identifier.scopusauthoridCheng, LYL=7403337122en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK

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