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Article: Blood vessel morphometry in human colorectal lesions

TitleBlood vessel morphometry in human colorectal lesions
Authors
Issue Date1995
PublisherHistology and Histopathology. The Journal's web site is located at http://www.hh.um.es
Citation
Histology And Histopathology, 1995, v. 10 n. 3, p. 589-596 How to Cite?
AbstractNeovascularisation in tumours of different cell origins has been well documented qualitatively. In this report, we have assessed vascular architecture in different pathological lesions of the colorectum by quantifying blood vessel parameters in order to detect subtle morphological changes using objective methods. Colorectal tissue samples were obtained from resected large bowels containing malignant tumours. Biopsies were taken from defined sites in the resected specimen and were classified as normal (N), potentially premalignant mucosa (PPM), adenomatous polyp (P) and adenocarcinoma (ADCA). All tissues were fixed in modified Karnovsky's fixative for 4 hrs and postfixed in 1% OsO4 for 1 hr. Samples were processed for EM under standardized procedures and embedded in Epon. 0.5 μm semithin sections from five patients per group were stained with toluidine blue. A multistage systematic sampling procedure was adopted. The inner outlines of all blood vessels in the lamina propria (LP) were digitised using a Zeiss VIDAS Image Analyzer at a final magnification of x 1,050. The area of the reference (LP) was also measured. No attempt was made to distinguish between the different types of vessel. The morphometric blood vessels parameters quantified were volume density (V(v)), numerical density (N(A)), length density (L(V)) and mean transverse sectional area (A). Statistically significant differences in Vv and A were detected between all groups except between N and PPM and between P and ADCA. No significant differences in N(A) and L(V) were present in any group comparisons. The mean values of all parameters were the highest in ADCA. Our results suggest that vasodilatation occurred in order to provide an increased supply of nutrients to support active growth and division of the transforming cells. Such vasodilatation might also reflect the inflammatory response to the presence of actively growing malignant cells since activated immune cells are able to release vasoactive substances.
Persistent Identifierhttp://hdl.handle.net/10722/67423
ISSN
2015 Impact Factor: 1.875
2015 SCImago Journal Rankings: 0.805
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorWhite, FHen_HK
dc.date.accessioned2010-09-06T05:55:01Z-
dc.date.available2010-09-06T05:55:01Z-
dc.date.issued1995en_HK
dc.identifier.citationHistology And Histopathology, 1995, v. 10 n. 3, p. 589-596en_HK
dc.identifier.issn0213-3911en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67423-
dc.description.abstractNeovascularisation in tumours of different cell origins has been well documented qualitatively. In this report, we have assessed vascular architecture in different pathological lesions of the colorectum by quantifying blood vessel parameters in order to detect subtle morphological changes using objective methods. Colorectal tissue samples were obtained from resected large bowels containing malignant tumours. Biopsies were taken from defined sites in the resected specimen and were classified as normal (N), potentially premalignant mucosa (PPM), adenomatous polyp (P) and adenocarcinoma (ADCA). All tissues were fixed in modified Karnovsky's fixative for 4 hrs and postfixed in 1% OsO4 for 1 hr. Samples were processed for EM under standardized procedures and embedded in Epon. 0.5 μm semithin sections from five patients per group were stained with toluidine blue. A multistage systematic sampling procedure was adopted. The inner outlines of all blood vessels in the lamina propria (LP) were digitised using a Zeiss VIDAS Image Analyzer at a final magnification of x 1,050. The area of the reference (LP) was also measured. No attempt was made to distinguish between the different types of vessel. The morphometric blood vessels parameters quantified were volume density (V(v)), numerical density (N(A)), length density (L(V)) and mean transverse sectional area (A). Statistically significant differences in Vv and A were detected between all groups except between N and PPM and between P and ADCA. No significant differences in N(A) and L(V) were present in any group comparisons. The mean values of all parameters were the highest in ADCA. Our results suggest that vasodilatation occurred in order to provide an increased supply of nutrients to support active growth and division of the transforming cells. Such vasodilatation might also reflect the inflammatory response to the presence of actively growing malignant cells since activated immune cells are able to release vasoactive substances.en_HK
dc.languageengen_HK
dc.publisherHistology and Histopathology. The Journal's web site is located at http://www.hh.um.esen_HK
dc.relation.ispartofHistology and Histopathologyen_HK
dc.subject.meshAdenocarcinoma - blood supply - ultrastructureen_HK
dc.subject.meshAdenoma - blood supply - ultrastructureen_HK
dc.subject.meshAgeden_HK
dc.subject.meshBiopsyen_HK
dc.subject.meshBlood Vessels - pathology - ultrastructureen_HK
dc.subject.meshColorectal Neoplasms - blood supply - ultrastructureen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImage Processing, Computer-Assisteden_HK
dc.subject.meshIntestinal Mucosa - blood supply - ultrastructureen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicroscopy, Electronen_HK
dc.subject.meshNeovascularization, Pathologic - pathologyen_HK
dc.subject.meshPrecancerous Conditions - pathologyen_HK
dc.titleBlood vessel morphometry in human colorectal lesionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0213-3911&volume=10&spage=589&epage=596&date=1995&atitle=Blood+vessel+morphometry+in+human+colorectal+lesionsen_HK
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid7579806-
dc.identifier.scopuseid_2-s2.0-0029047413en_HK
dc.identifier.hkuros8493en_HK
dc.identifier.volume10en_HK
dc.identifier.issue3en_HK
dc.identifier.spage589en_HK
dc.identifier.epage596en_HK
dc.identifier.isiWOS:A1995RP97900007-
dc.publisher.placeSpainen_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.scopusauthoridWhite, FH=7202578907en_HK

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