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Article: Mouse PSP94 expression is prostate tissue-specific as demonstrated by a comparison of multiple antibodies against recombinant proteins

TitleMouse PSP94 expression is prostate tissue-specific as demonstrated by a comparison of multiple antibodies against recombinant proteins
Authors
Issue Date2003
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
Citation
Journal Of Cellular Biochemistry, 2003, v. 88 n. 5, p. 999-1011 How to Cite?
AbstractProstate tissue-specific gene expression is crucial for driving potentially therapeutic genes to target specifically to the prostate. Prostate secretory protein of 94 amino acids (PSP94), also known as β-MSP (microseminoprotein), is one of the three most abundant secretory proteins of the prostate gland, and is generally considered to be prostate tissue-specific. We have previously demonstrated that the expression of the rat PSP94 gene is strictly prostate tissue-specific by an antibody against a recombinant rat PSP94. In order to study prostate targeting utilizing the PSP94 gene in a mouse pre-clinical experimental model, we need to establish antibodies against mouse PSP94 to confirm if it is prostate tissue-specific as well. In this study, firstly we raised a polyclonal antibody against a recombinant glutathione-S-transferase-(GST-) mouse mature form of PSP94. However, it showed very poor immunoreactivity against prostate tissue PSP94 as tested in Western blotting experiments. Neither antibodies against rat PSP94 nor mouse PSP94 showed significant cross-reactivity. Thus a second antibody was established against a recombinant mouse mature PSP94 containing N-terminal polyhistidines, and stronger immunoreactivity against mouse prostate tissue PSP94 was identified in Western blotting experiments. Both of these antibodies showed immunohistochemical reactivity, while the latter showed stronger reactivity in IHC when tested with different fixatives. By studying tissue distribution, we demonstrated that, as with rat PSP94, mouse PSP94 is strictly prostate tissue-specific in experiments of both Western blotting and immunohistochemistry (IHC). This conclusion was also derived from a comparison among antibodies against human, rat, and mouse PSP94, showing very different immunoreactivities in Western blotting and IHC. Finally, a competitive assay between different species was performed. We demonstrated that antibodies against PSP94 from different species (human, primate, rodents) have poor cross-reactivities. These observations also indicate that the PSP94 gene is a rapidly evolving gene in all species. Results from this study have led to the possibility of utilizing PSP94 as a targeting agent specifically to the prostate in a mouse experimental model. ©2003 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/67418
ISSN
2015 Impact Factor: 3.446
2015 SCImago Journal Rankings: 1.520
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorThota, Aen_HK
dc.contributor.authorKarajgikar, Men_HK
dc.contributor.authorDuan, Wen_HK
dc.contributor.authorGabril, MYen_HK
dc.contributor.authorChan, FLen_HK
dc.contributor.authorWong, YCen_HK
dc.contributor.authorSakai, Hen_HK
dc.contributor.authorChin, JLen_HK
dc.contributor.authorMoussa, Men_HK
dc.contributor.authorXuan, JWen_HK
dc.date.accessioned2010-09-06T05:54:58Z-
dc.date.available2010-09-06T05:54:58Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Cellular Biochemistry, 2003, v. 88 n. 5, p. 999-1011en_HK
dc.identifier.issn0730-2312en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67418-
dc.description.abstractProstate tissue-specific gene expression is crucial for driving potentially therapeutic genes to target specifically to the prostate. Prostate secretory protein of 94 amino acids (PSP94), also known as β-MSP (microseminoprotein), is one of the three most abundant secretory proteins of the prostate gland, and is generally considered to be prostate tissue-specific. We have previously demonstrated that the expression of the rat PSP94 gene is strictly prostate tissue-specific by an antibody against a recombinant rat PSP94. In order to study prostate targeting utilizing the PSP94 gene in a mouse pre-clinical experimental model, we need to establish antibodies against mouse PSP94 to confirm if it is prostate tissue-specific as well. In this study, firstly we raised a polyclonal antibody against a recombinant glutathione-S-transferase-(GST-) mouse mature form of PSP94. However, it showed very poor immunoreactivity against prostate tissue PSP94 as tested in Western blotting experiments. Neither antibodies against rat PSP94 nor mouse PSP94 showed significant cross-reactivity. Thus a second antibody was established against a recombinant mouse mature PSP94 containing N-terminal polyhistidines, and stronger immunoreactivity against mouse prostate tissue PSP94 was identified in Western blotting experiments. Both of these antibodies showed immunohistochemical reactivity, while the latter showed stronger reactivity in IHC when tested with different fixatives. By studying tissue distribution, we demonstrated that, as with rat PSP94, mouse PSP94 is strictly prostate tissue-specific in experiments of both Western blotting and immunohistochemistry (IHC). This conclusion was also derived from a comparison among antibodies against human, rat, and mouse PSP94, showing very different immunoreactivities in Western blotting and IHC. Finally, a competitive assay between different species was performed. We demonstrated that antibodies against PSP94 from different species (human, primate, rodents) have poor cross-reactivities. These observations also indicate that the PSP94 gene is a rapidly evolving gene in all species. Results from this study have led to the possibility of utilizing PSP94 as a targeting agent specifically to the prostate in a mouse experimental model. ©2003 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503en_HK
dc.relation.ispartofJournal of Cellular Biochemistryen_HK
dc.rightsJournal of Cellular Biochemistry. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntibodies - immunologyen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCross Reactionsen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshFixativesen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshGene Products, nefen_HK
dc.subject.meshGene Therapyen_HK
dc.subject.meshGenetic Vectorsen_HK
dc.subject.meshGlutathione Transferaseen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshProstate - metabolismen_HK
dc.subject.meshProstatic Neoplasms - genetics - therapyen_HK
dc.subject.meshProstatic Secretory Proteins - biosynthesis - genetics - immunologyen_HK
dc.subject.meshRecombinant Fusion Proteins - genetics - immunologyen_HK
dc.titleMouse PSP94 expression is prostate tissue-specific as demonstrated by a comparison of multiple antibodies against recombinant proteinsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0730-2312&volume=88&spage=999&epage=1011&date=2003&atitle=Mouse+PSP94+expression+is+prostate+tissue-specific+as+demonstrated+by+a+comparison+of+multiple+antibodies+against+recombinant+proteinsen_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jcb.10425en_HK
dc.identifier.pmid12616537-
dc.identifier.scopuseid_2-s2.0-0037377168en_HK
dc.identifier.hkuros88940en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037377168&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume88en_HK
dc.identifier.issue5en_HK
dc.identifier.spage999en_HK
dc.identifier.epage1011en_HK
dc.identifier.isiWOS:000181601700013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridThota, A=6508315515en_HK
dc.identifier.scopusauthoridKarajgikar, M=6504570491en_HK
dc.identifier.scopusauthoridDuan, W=8323784800en_HK
dc.identifier.scopusauthoridGabril, MY=6507772663en_HK
dc.identifier.scopusauthoridChan, FL=7202586505en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK
dc.identifier.scopusauthoridSakai, H=35394282200en_HK
dc.identifier.scopusauthoridChin, JL=7201494674en_HK
dc.identifier.scopusauthoridMoussa, M=7103250154en_HK
dc.identifier.scopusauthoridXuan, JW=7004718061en_HK
dc.identifier.citeulike3906276-

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