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Article: Immortalization of human prostate epithelial cells by HPV 16 E6/E7 open reading frames

TitleImmortalization of human prostate epithelial cells by HPV 16 E6/E7 open reading frames
Authors
KeywordsCancer progression
Cytokeratin
HPV 16 E6/E7
Human prostate epithelial cell
Immortalization
Telomerase
Issue Date1999
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 1999, v. 40 n. 3, p. 150-158 How to Cite?
AbstractBACKGROUND. The exact pathogenesis for prostate cancer is not known. Progress made in prostate cancer research has been slow, largely due to the lack of suitable in vitro models. Here, we report our work on the immortalization of a human prostate epithelial cell line and show that it can be used as a model to study prostate tumorigenesis. METHODS. Replication- defective retrovirus harboring the human papillomavirus (HPV) type 16 E6 and E7 open reading frames was used to infect primary human prostate epithelial cells. Polymerase chain reaction, followed by Southern hybridization for the HPV 16 E6/E7, Western blot for prostatic acid phosphatase, telomeric repeat amplification protocol assay for telomerase activity, two-dimensional gels for cytokeratins, and cytogenetic analysis were undertaken to characterized the infected cells. RESULTS. The retrovirus-infected cell line, HPr-1, continued to grow in culture for more than 80 successive passages. Normal primary cells failed to proliferate after passage 6. HPr-1 cells bore close resemblance to normal primary prostate epithelial cells, both morphologically and biochemically. However, they possessed telomerase activity and proliferated indefinitely. Cytogenetic analysis of HPr-1 cells revealed a human male karyotype with clonal abnormalities and the appearance of multiple double minutes. CONCLUSIONS. The HPr-1 cells expressed prostatic acid phosphatase and cytokeratins K8 and K18, proving that they were prostate epithelial cells. They were benign in nude mice tumor formation and soft agar colony formation assay. The HPr-1 cell line is an in vitro representation of early prostate neoplastic progression.
Persistent Identifierhttp://hdl.handle.net/10722/67387
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChoo, CKen_HK
dc.contributor.authorLing, MTen_HK
dc.contributor.authorChan, KWen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorZheng, Zen_HK
dc.contributor.authorZhang, Den_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorWong, YCen_HK
dc.date.accessioned2010-09-06T05:54:41Z-
dc.date.available2010-09-06T05:54:41Z-
dc.date.issued1999en_HK
dc.identifier.citationProstate, 1999, v. 40 n. 3, p. 150-158en_HK
dc.identifier.issn0270-4137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67387-
dc.description.abstractBACKGROUND. The exact pathogenesis for prostate cancer is not known. Progress made in prostate cancer research has been slow, largely due to the lack of suitable in vitro models. Here, we report our work on the immortalization of a human prostate epithelial cell line and show that it can be used as a model to study prostate tumorigenesis. METHODS. Replication- defective retrovirus harboring the human papillomavirus (HPV) type 16 E6 and E7 open reading frames was used to infect primary human prostate epithelial cells. Polymerase chain reaction, followed by Southern hybridization for the HPV 16 E6/E7, Western blot for prostatic acid phosphatase, telomeric repeat amplification protocol assay for telomerase activity, two-dimensional gels for cytokeratins, and cytogenetic analysis were undertaken to characterized the infected cells. RESULTS. The retrovirus-infected cell line, HPr-1, continued to grow in culture for more than 80 successive passages. Normal primary cells failed to proliferate after passage 6. HPr-1 cells bore close resemblance to normal primary prostate epithelial cells, both morphologically and biochemically. However, they possessed telomerase activity and proliferated indefinitely. Cytogenetic analysis of HPr-1 cells revealed a human male karyotype with clonal abnormalities and the appearance of multiple double minutes. CONCLUSIONS. The HPr-1 cells expressed prostatic acid phosphatase and cytokeratins K8 and K18, proving that they were prostate epithelial cells. They were benign in nude mice tumor formation and soft agar colony formation assay. The HPr-1 cell line is an in vitro representation of early prostate neoplastic progression.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_HK
dc.relation.ispartofProstateen_HK
dc.rightsThe Prostate. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectCancer progressionen_HK
dc.subjectCytokeratinen_HK
dc.subjectHPV 16 E6/E7en_HK
dc.subjectHuman prostate epithelial cellen_HK
dc.subjectImmortalizationen_HK
dc.subjectTelomeraseen_HK
dc.subject.meshAcid Phosphatase - analysisen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCell Transformation, Neoplasticen_HK
dc.subject.meshChromosome Aberrationsen_HK
dc.subject.meshEpithelial Cells - cytologyen_HK
dc.subject.meshGenetic Vectorsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKaryotypingen_HK
dc.subject.meshKeratins - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshOncogene Proteins, Viral - geneticsen_HK
dc.subject.meshOpen Reading Framesen_HK
dc.subject.meshPapillomaviridae - geneticsen_HK
dc.subject.meshPapillomavirus E7 Proteinsen_HK
dc.subject.meshProstate - cytologyen_HK
dc.subject.meshRepressor Proteinsen_HK
dc.subject.meshRetroviridaeen_HK
dc.subject.meshTelomerase - geneticsen_HK
dc.subject.meshTransplantation, Heterologousen_HK
dc.subject.meshX Chromosomeen_HK
dc.subject.meshY Chromosomeen_HK
dc.titleImmortalization of human prostate epithelial cells by HPV 16 E6/E7 open reading framesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-4137&volume=40&issue=3&spage=150&epage=158&date=1999&atitle=Immortalization+of+human+prostate+epithelial+cells+by+HPV+16+E6/E7+open+reading+framesen_HK
dc.identifier.emailLing, MT:patling@hkucc.hku.hken_HK
dc.identifier.emailChan, KW:hrmtckw@hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.authorityLing, MT=rp00449en_HK
dc.identifier.authorityChan, KW=rp00330en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1097-0045(19990801)40:3<150::AID-PROS2>3.0.CO;2-7en_HK
dc.identifier.pmid10398276-
dc.identifier.scopuseid_2-s2.0-0032798852en_HK
dc.identifier.hkuros47668en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032798852&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue3en_HK
dc.identifier.spage150en_HK
dc.identifier.epage158en_HK
dc.identifier.isiWOS:000081471000002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChoo, CK=35866260100en_HK
dc.identifier.scopusauthoridLing, MT=7102229780en_HK
dc.identifier.scopusauthoridChan, KW=16444133100en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridZheng, Z=36792437600en_HK
dc.identifier.scopusauthoridZhang, D=7405361705en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK

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